L-Ascorbic Acid Increases Intracellular Tetrahydrobiopterin Via A Chemical Stabilization and Potentiates Nitric Oxide Synthesis in Endothelial Cells

Heller, Regine; Unbehaun, Anett; Schellenberg, Berit; Mayer, Bernd; Werner-Felmayer, Gabriele; Werner, Ernst R.

doi:10.1007/978-1-4615-0945-5_44

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Ascorbic acid improves endothelial function by also stabilizing tetrahydrobiopterin (37) and alpha-tocopherol (38), which are cofactors for the production of NO. Without the production of these cofactors, the production of NO would be reduced.…”

Section: Antioxidant Supplementationmentioning

confidence: 99%

Effects of antioxidants on contracting spinotrapezius muscle microvascular oxygenation and blood flow in aged rats

Herspring¹,

Ferreira²,

Copp³

et al. 2008

Journal of Applied Physiology

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Aged rats exhibit a decreased muscle microvascular O(2) partial pressure (Pmv(O(2))) at rest and during contractions compared with young rats. Age-related reductions in nitric oxide bioavailability due, in part, to elevated reactive O(2) species, constrain muscle blood flow (Qm). Antioxidants may restore nitric oxide bioavailability, Qm, and ameliorate the reduced Pmv(O(2)). We tested the hypothesis that antioxidants would elevate Qm and, therefore, Pmv(O(2)) in aged rats. Spinotrapezius muscle Pmv(O(2)) and Qm were measured, and oxygen consumption (Vm(O(2))) was estimated in anesthetized male Fisher 344 x Brown Norway hybrid rats at rest and during 1-Hz contractions, before and after antioxidant intravenous infusion (76 mg/kg vitamin C and 52 mg/kg tempol). Before infusion, contractions evoked a biphasic Pmv(O(2)) that fell from 30.6 +/- 0.9 Torr to a nadir of 16.8 +/- 1.2 Torr with an "undershoot" of 2.8 +/- 0.7 Torr below the subsequent steady-state (19.7 +/- 1.2 Torr). The principal effect of antioxidants was to elevate baseline Pmv(O(2)) from 30.6 +/- 0.9 to 35.7 +/- 0.8 Torr (P < 0.05) and reduce or abolish the undershoot (P < 0.05). Antioxidants reduced Qm and Vm(O(2)) during contractions (P < 0.05), while decreasing force production 16.5% (P < 0.05) and elevating the force production-to-Vm(O(2)) ratio (0.92 +/- 0.03 to 1.06 +/- 0.6, P < 0.05). Thus antioxidants increased Pmv(O(2)) by altering the balance between muscle O(2) delivery and Vm(O(2)) at rest and during contractions. It is likely that this effect arose from antioxidants reducing myocyte redox below the level optimal for contractile performance and directly (decreased tension) or indirectly (altered balance of vasoactive mediators) influencing O(2) delivery and Vm(O(2)).

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Section: Antioxidant Supplementationmentioning

confidence: 99%

Effects of antioxidants on contracting spinotrapezius muscle microvascular oxygenation and blood flow in aged rats

Herspring¹,

Ferreira²,

Copp³

et al. 2008

Journal of Applied Physiology

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“…1,15 Impaired activity of EPCs in the blood is associated with cardiovascular risk factors because It reflects impaired regenerative potential. 16 In this research, Allicin at a dose of 100 mcg/mL, 200 mcg/mL, and 400 mcg/mL was shown to improve EPCs migration in a dose-dependent manner compared to the untreated group. Similarly, Allicin has been proven to enhance the proliferation and migration of cardiac microvascular endothelial cells.…”

Section: Discussionmentioning

confidence: 76%

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

Oktaviono¹,

Amadis²,

Al-Farabi³

2020

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MATERIAL AND METHODS Allicin and Vitamin C preparation Garlic extract contains Allicin (ChemFaces ®) 98% was dissolved in dimethyl sulphoxide (DMSO). Vitamin C powder (Sigma Aldrich, USA) was dissolved in double-distilled water and diluted with culture medium to acquire the dose of 250 mcg/mL. Sample criteria The blood sample was acquired from eight CAD patients in Dr Soetomo General Hospital who fulfilled the inclusion criteria, which are: male, aged ABSTRACT Endothelial Progenitor Cells (EPCs) have an important role in endothelial dysfunction repairment through neovasculogenesis and cardiac myocytes regeneration. However, EPCs migration is greatly reduced in the patient with Coronary Artery Disease (CAD). Allicin and Vitamin C are hypothesized to improve EPCs migration due to its antioxidant properties. Objective: To investigate the effect of Allicin and its combination with Vitamin C in EPCs migration of CAD patients. Material and Method: Mononuclear cells were isolated from CAD patients and cultured on fibronectin-coated plates with colony-forming unit Hill medium. The cells were divided into untreated (control), Allicin treatment (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ ml), and each dose of Allicin combined with 250 mcg/mL of Vitamin C. EPCs migration was assessed with Transwell Migration Assay Kit and evaluated by using statistical tests. Results: This research shows that EPC migration was significantly higher in the treatment. Allicin at all dose (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ml) and its combination with 250 mcg/mL of vitamin C compared to untreated group (p<0.05). Allicin increase EPCs migration in a dosedependent manner. However, the only combination of 400 mcg/ml Allicin with 250 mcg/mL of vitamin C which has significantly higher EPCs migration compared to Allicin treatment alone. Conclusion: Allicin improves EPCs migration in a dose-dependent manner. Improvement of the migration only observed on the Allicin dose 400 mcg/ml with Vitamin C.

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L-Ascorbic Acid Increases Intracellular Tetrahydrobiopterin Via A Chemical Stabilization and Potentiates Nitric Oxide Synthesis in Endothelial Cells

Cited by 2 publications

References 9 publications

Effects of antioxidants on contracting spinotrapezius muscle microvascular oxygenation and blood flow in aged rats

Effects of antioxidants on contracting spinotrapezius muscle microvascular oxygenation and blood flow in aged rats

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

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