L-Arginine Supplementation for Nulliparous Sows during the Last Third of Gestation

Abstract

We evaluated the effects of L-arginine supplementation during the last third of gestation on molecular mechanisms related to skeletal muscle development of piglets and litter traits at birth. Twenty-three nulliparous sows averaging 205.37 ± 11.50 kg of body weight were randomly assigned to the following experimental treatments: control (CON), where pregnant sows were fed diets to meet their nutritional requirements; arginine (ARG), where sows where fed CON + 1.0% L-arginine. Skeletal muscle from piglets born from sows from ARG group had greater mRNA expression of MYOD (p = 0.043) and MYOG (p ≤ 0.01), and tended to present greater mRNA expression (p = 0.06) of IGF-2 gene compared to those born from CON sows. However, there were no differences (p > 0.05) in the histomorphometric variables of fetuses’ skeletal muscle. The total weight of born piglets, total weight of born alive piglets, piglet weight at birth, coefficient of variation of birth weight, and the incidence of intrauterine growth restriction (IUGR) piglets did not differ between groups. No stillborn piglets (p < 0.01) were verified in the ARG sows compared to CON group. The blood levels of estradiol (p = 0.035) and urea (p = 0.03) were higher in ARG sows compared to those from the CON group. In summary, our data show that arginine supplementation of nulliparous sows at late gestation enhance mRNA expression of key myogenic regulatory factors, which likely contribute to improve animal growth rates in later stages of development.

Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development

“…Restricted maternal nutrition will reduce the myogenic regulatory factor expression [ 27 ]. On the other hand, there were studies that found that adding L-arginine or lactic acid to the diet would increase the gene expression of MYOD in muscles, thereby promoting muscle development [ 28 , 29 ]. The MSTN gene expression was down-regulated at weaning and up-regulated during the finishing period due to a maternal low-protein diet, indicating that the expression pattern of MSTN was stage-specific [ 30 ].…”