‘Kwanzan Stunting’ syndrome: Detection and molecular characterization of an Italian isolate of Little cherry virus 1

Matić, S.; Minafra, A.; Sánchez‐Navarro, J. A.; Pallás, Vicente; Myrta, A.; Martelli, G. P.

doi:10.1016/j.virusres.2009.03.005

Cited by 22 publications

(48 citation statements)

References 21 publications

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“…Distinct fruit symptoms involving a dramatic reduction of fruit size, colour and taste occur in susceptible sweet cherry cultivars, whereas other cultivars show only moderate fruit deformations or are tolerant. Different isolates of LChV-1 have also been associated with various other disorders such as Kwanzan stunting syndrome and Shirofugen stunt disease (Matic et al, 2009;Candresse et al, 2013). Recently, LChV-1 was reported in other Prunus species such as almond, peach and plum, although without inducing any obvious symptoms (Matic et al, 2007(Matic et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

“…Different isolates of LChV-1 have also been associated with various other disorders such as Kwanzan stunting syndrome and Shirofugen stunt disease (Matic et al, 2009;Candresse et al, 2013). Recently, LChV-1 was reported in other Prunus species such as almond, peach and plum, although without inducing any obvious symptoms (Matic et al, 2007(Matic et al, , 2009. The virus is graft-transmissible, but no vector is known and it is spread with infected plant material.…”

Section: Introductionmentioning

confidence: 99%

“…The complete genome sequences of three LChV-1 isolates have been determined (Keim-Konrad & Jelkmann, 1996;Jelkmann et al, 1997;Matic et al, 2009;Candresse et al, 2013). The RNA genome of the virus, c. 17 kb, is organized in eight open reading frames (ORFs) that potentially encode the conserved replication domains of papain-like protease, methyltransferase and helicase (ORF1a), the RNA-dependent RNA polymerase (RdRp; ORF1b), a small hydrophobic protein (p4; ORF2), a 70 kDa heat-shock protein homologue (HSP70 h; ORF3), a polypeptide of 61 kDa (p61; ORF4), the coat protein (CP; ORF5) and CP minor (CPm; ORF6) proteins and two polypeptides with currently unknown function of 21 kDa (p21; ORF7) and 27 kDa (p27; ORF8) .…”

Section: Introductionmentioning

confidence: 99%

“…The RNA genome of the virus, c. 17 kb, is organized in eight open reading frames (ORFs) that potentially encode the conserved replication domains of papain-like protease, methyltransferase and helicase (ORF1a), the RNA-dependent RNA polymerase (RdRp; ORF1b), a small hydrophobic protein (p4; ORF2), a 70 kDa heat-shock protein homologue (HSP70 h; ORF3), a polypeptide of 61 kDa (p61; ORF4), the coat protein (CP; ORF5) and CP minor (CPm; ORF6) proteins and two polypeptides with currently unknown function of 21 kDa (p21; ORF7) and 27 kDa (p27; ORF8) . The fully sequenced isolates ITMAR (Matic et al, 2009) and UW2 share 94% nucleotide identity while V2356 (Candresse et al, 2013) is a diverse isolate showing an overall nucleotide divergence of 23Á8 and 23Á5% from ITMAR and UW2, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…So far the molecular variability of LChV-1 has been barely studied, with most of the past efforts focusing on short fragments of the RdRp, the CPm and the 3 0 end of ORF8 (Bajet et al, 2008;Matic et al, 2009;Candresse et al, 2013). Phylogenetic relationships between LChV-1 isolates obtained from remote geographical regions and/ or originating from different Prunus trees have shown that classification according to geographic origin or host is probably not feasible (Matic et al, 2009;Candresse et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Insights into the genetic diversity and evolution of Little cherry virus 1

et al. 2014

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Little cherry virus 1 (LChV-1), a member of the recently proposed genus Velarivirus, is a sweet cherry pathogen that has been recently reported to infect other Prunus species and is associated with various plant disorders. In this work the incidence of the virus on its putative hosts and possible mechanisms driving its evolution were investigated. Due to problems encountered with LChV-1 detection, a new nested RT-PCR assay was developed and applied. The virus was found to be prevalent in cherry plantations in Greece and only occasionally detected in other Prunus species. Sequences corresponding to the partial RNA-dependent RNA polymerase (RdRp), heat-shock protein homologue (HSP70h) and coat protein (CP) genes were determined from Greek LChV-1 isolates originating from different hosts; these were analysed, along with published homologous genomic regions from other isolates. Phylogenetic analysis of the three genes revealed the segregation of four evolutionary distinct groups showing no host or geography-based clustering. Mean genetic distances among the four groups were high with the CP region showing the highest divergence, although intragroup variability levels were low. Nevertheless, estimations of the mean ratio of nonsynonymous substitutions per synonymous site to synonymous substitutions per synonymous site (dN/dS) for the partial RdRp, HSP70h and CP indicated that these genomic regions are under negative selection pressure. Interestingly, a recombination event was identified at the 3 0 end of RdRp on a Greek virus isolate, thus highlighting the role of this mechanism in the evolutionary history of LChV-1.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Insights into the genetic diversity and evolution of Little cherry virus 1

et al. 2014

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Prunus serrulata (Oriental flowering cherry)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

et al. 2016

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Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76%, 76%, and 78% to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.

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‘Kwanzan Stunting’ syndrome: Detection and molecular characterization of an Italian isolate of Little cherry virus 1

Cited by 22 publications

References 21 publications

Insights into the genetic diversity and evolution of Little cherry virus 1

Insights into the genetic diversity and evolution of Little cherry virus 1

Prunus serrulata (Oriental flowering cherry)

Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

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