Kurtzmanomyces tardus sp. nov., a new anamorphic yeast species of basidiomycetous affinity

Abstract

A new yeast species of basidiomycetous affinity Kurtzmanomyces tardus was isolated from contaminated demineralized water. It differs from K. nectairii, the only other Kurtzmanomyces species so far described, in its carbon assimilation pattern and low DNA-DNA homology (2.3% +/- 2.1).

Characterisation and classification of phylloplane yeasts from Portugal related to the genus and description of five novel species

“…Gel electrophoresis images were acquired with the Kodak Digital Science 1D Image Analysis Software. The protocols for determination of the molar content of G+C of genomic DNA from thermal denaturation curves and of DNA/DNA homologies from reassociation kinetics using a Gilford Response UV-VIS spectrophotometer followed Gime ¤nez-Jurado et al [24]. PCR ampli¢cation prior to sequencing employed forward primer NS7 (5P GAG GCA ATA ACA GGT CTG TGA TGC) or ITS5 (5P GGA AGT AAA AGT CGT AAC AAG G) and reverse primer LR6 (5P CGC CAG TTC TGC TTA CC) using a Uno II Thermal Cycler (Biometra, Go « ttingen, Germany) and the resulting amplicon was puri¢ed with the GFX Band Puri-¢cation Kit (Amersham Biosciences).…”

Candida tartarivorans sp. nov., an anamorphic ascomycetous yeast with the capacity to degrade L(+)- and meso-tartaric acid.

“…The identification methods, including determination of molar content of GjC in nDNA (thermal denaturation method ; four determinations ; reference : nDNA of Candida parapsilosis CBS 604 -GjC l 40n5 mol %), followed Gime! nez- Jurado et al (1990). In addition to assessing the utilization of standard carbon compounds in liquid media, several aldaric acids (Fonseca, 1992) and aromatic compounds (Sampaio, 1994) were tested using the techniques of Yarrow (1998).…”

The phylogenetic relationship of Kurtzmanomyces tardus Gimenez-Jurado et van Uden (Cryptococcaceae) based on the partial sequences of 18S and 26S ribosomal RNAs.

“…Kurtzmanomyces tardus Gimenez-Jurado et van Uden was recently described as a second species of the genus Kurtzmanomyces Yamada, Itoh, Kawasaki, Banno et Nakase (Cryptococcaceae) (2). Yamada et al (8) and Gueho et al (3) previously determined the partial sequences of 18S and 26S (25S) rRNAs of the strains of species in the stalked conidium-forming anamorphic yeast genera and proved the Q10-equipped, xylose-negative, monotypic genus Kurtzmanomyces to be phylogenetically separable at the generic level.…”

“…Sterigmatomyces halophilus and F. polyborus were linked to K. tardus at 5 and 13 base differences, respectively. Kurtzmanomyces tardus has morphological, physiological, biochemical, and chemotaxonomic characters similar to K. nectairei, the type species of the genus Kurtzmanomyces (2). The phenotypic discriminations between the two species are in the assimilation of sucrose, trehalose, succinate, and ethanol, growth at 30°C, and production of true mycelium (2).…”

“…Kurtzmanomyces tardus has morphological, physiological, biochemical, and chemotaxonomic characters similar to K. nectairei, the type species of the genus Kurtzmanomyces (2). The phenotypic discriminations between the two species are in the assimilation of sucrose, trehalose, succinate, and ethanol, growth at 30°C, and production of true mycelium (2). However, the present experiment has demonstrated that K. tardus is phylogenetically separable at the generic level from any other presently accepted stalked conidium-forming anamorphic yeast species, and is accommodated in its own genus.…”