Kumar, Anil: Development of Two-Dimensional NMR-My Perception

Kumar, A. Muneesh

doi:10.1002/9780470034590.emrhp0103

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“…Two-dimensional proton n.m.r. [COSY and SUPERCOSY (27)(28)(29)] spectra were recorded on a Bruker WH-270 spectrometer equipped with an Aspect-2000 computer system. 512 t, values with 64 transients for each t, value were collected.…”

Section: Methodsmentioning

confidence: 99%

Molecular conformation of alamethicin in dimethylsulfoxide solution

Chandrasekhar

Das

Kumar

et al. 1988

International Journal of Peptide and Protein Research

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The solution conformation of alamethicin, a 20‐residue antibiotic peptide, has been investigated using two‐dimensional n.m.r. spectroscopy. Complete proton resonance assignments of this peptide have been carried out using COSY, SUPERCOSY, RELAY COSY and NOESY two‐dimensional spectroscopies. Observation of a large number of nuclear Overhauser effects between sequential backbone amide protons, between backbone amide protons and CβH protons of preceding residues and extensive intramolecular hydrogen bonding patterns of NH protons has established that this polypeptide is in a largely helical conformation. This result is in conformity with earlier reported solid state X‐ray results and a recent n.m.r. study in methanol solution (Esposito et al. (1987) Biochemistry26, 1043‐1050) but is at variance with an earlier study which favored an extended conformation for the C‐terminal half of alamethicin (Bannerjee et al. (1983) J. Mol. Biol.165, 757‐775).

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Section: Methodsmentioning

confidence: 99%