KRAS and BRAF mutation analysis can be reliably performed on aspirated cytological specimens of metastatic colorectal carcinoma

Pang, Ning-Qi; Nga, Min‐En; Chin, Sze Yung; Ismail, T.; Lim, Guat Ling; Soong, Richie; Salto-Tellez, Manuel

doi:10.1111/j.1365-2303.2010.00812.x

Cited by 28 publications

(22 citation statements)

References 29 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PCR amplification and subsequent direct sanger sequencing of BRAF exon 15 were carried out according to published protocols [11]. Our test validation of the BRAF exon 15 detection by direct sequencing had shown 100% sensitivity and specificity, with a lower limit of detection set to 30% of mutated cells in a background of 70% of non-mutated cells [12]. For KIT exons 9 and 11 mutation analysis, polymerase chain reaction (PCR) amplification of 100 ng of genomic DNA was set up as described previously [13].…”

Section: Methodsmentioning

confidence: 99%

Biphasic Low-Grade Nasopharyngeal Papillary Adenocarcinoma with a Prominent Spindle Cell Component: Report of a Case Localized to the Posterior Nasal Septum

Petersson

Pang

Loke³

et al. 2011

Head and Neck Pathol

View full text Add to dashboard Cite

A case (female, 39 years of) of thyroid-like nasopharyngeal low-grade papillary adenocarcinoma with a significant spindle cell component is presented. The tumor was located on the posterior nasal septum. The spindle cells displayed nuclear features very much similar to the epithelial component and the two cell types merged imperceptibly. Immunohistochemically, the neoplastic cells (including the spindle cell component) were strongly and diffusely positive for TTF-1, cytokeratins (AE1-3), cytokeratin 19 and vimentin. C-kit immunohistochemistry showed diffuse mild to moderate membranous positivity with focal areas displaying moderate to strong immunoreactivity. EMA was strongly positive in the epithelial component with membranous and cytoplasmic reactivity whereas the spindle cell component was weakly although diffusely positive. Carcinoembryonic antigen, calcitonin, chromogranin A, S100-protein, thyroglobulin, cdx2 and p63 were negative. The proliferative activity (Mib-1/Ki-67) was low; 3-4%. In the molecular genetic study we found no mutations at position 1799 (exon 15) in the BRAF-gene, (BRAFV600E) or in exons 9 and 11 of the KIT-gene.

show abstract

Section: Methodsmentioning

confidence: 99%

Biphasic Low-Grade Nasopharyngeal Papillary Adenocarcinoma with a Prominent Spindle Cell Component: Report of a Case Localized to the Posterior Nasal Septum

Petersson

Pang

Loke³

et al. 2011

Head and Neck Pathol

View full text Add to dashboard Cite

show abstract

“…(v-raf murine sarcoma viral oncogene B1) status with high concordance to primary lesions (17,19,(25)(26)(27). While conventional assays identify patients as RAS mutant when a mutation is detected in >10% of reads sequenced, sensitivities approaching 0.1% are now possible (28).…”

Section: Pik3camentioning

confidence: 99%

Current companion diagnostics in advanced colorectal cancer; getting a bigger and better piece of the pie

Loree

Kopetz

Raghav

2017

J. Gastrointest. Oncol.

View full text Add to dashboard Cite

While the treatment of colorectal cancer continues to rely heavily on conventional cytotoxic therapy, an increasing number of targeted agents are under development. Many of these treatments require companion diagnostic tests in order to define an appropriate population that will derive benefit. In addition, a growing number of biomarkers provide prognostic information about a patient's malignancy. As we learn more about these biomarkers and their assays, selecting the appropriate companion diagnostic becomes increasingly important. In the case of many biomarkers, there are numerous assays which could provide the same information to a treating physician, however each assay has strengths and weaknesses. Institutions must balance cost, assay sensitivity, turn-around time, and labor resources when selecting which assay to offer. In this review we will discuss the current state of companion diagnostics available in metastatic colorectal cancer and explore emerging biomarkers and their assays. We will focus on KRAS, BRAF, HER2, and PIK3CA testing, as well as microsatellite stability assessment and multigene panels.

show abstract

“…In this domain, neoplastic lesions of lymph nodes, soft tissue sarcomas, gastrointestinal stromal tumors and colon cancers have been studied, and some results have shown the use of cytological material for the management of patients with these malignancies [58,59,60,61,62,63]. …”

Section: Body Fluidsmentioning

confidence: 99%

“…However, there are some KRAS wild-type cases that are still unresponsive to anti- EGFR therapies. Some authors have shown that this unresponsiveness could be due to the presence of a discrepancy in KRAS status between the primary tumor and its metastases [62,63]. …”

Section: Primary Tumors and Metastasesmentioning

confidence: 99%

Is Liquid-Based Cytology the Magic Bullet for Performing Molecular Techniques?

Abedi-Ardekani

Vielh²

2014

Acta Cytologica

View full text Add to dashboard Cite

Objective: The role of pathology has evolved from the first microscopic definitions of diseases by Virchow to the new concept of molecular cytopathology. The management of diseases is now a multidisciplinary approach with the translation of morphological, imagery and molecular findings to therapeutic protocols. Obtaining the most reliable diagnostic material is the essential part of the medical management of patients. Study Design: Here, we try to gain a concise insight into the available data regarding the role of cytology in the application of molecular techniques, focusing on cancer cytopathology. Results: Obtaining cytological material is now feasible by different methods, and in some cases it is the only possible approach to a lesion which is not easily accessible for tissue sampling. The methods of obtaining cytological material have evolved in recent years in parallel with rapid advances in high-throughput molecular techniques, opening new windows for the diagnosis and management of diseases. Conclusions: Different kinds of cytological material are reliable for the application of molecular techniques. Cytological material obtained in a liquid base has advantages such as the better preservation of cytomorphological features and the use of the remaining liquid for nucleic acid extraction even after long storage and the application of molecular methods.

show abstract

KRAS and BRAF mutation analysis can be reliably performed on aspirated cytological specimens of metastatic colorectal carcinoma

Abstract: A range of cytological samples are suitable for KRAS and BRAF mutation testing, be it from previously stained preparations or cell blocks. These samples would be highly valuable in cases where cytological samples are the only material available for mutation testing.

Cited by 28 publications

References 29 publications

Biphasic Low-Grade Nasopharyngeal Papillary Adenocarcinoma with a Prominent Spindle Cell Component: Report of a Case Localized to the Posterior Nasal Septum

Biphasic Low-Grade Nasopharyngeal Papillary Adenocarcinoma with a Prominent Spindle Cell Component: Report of a Case Localized to the Posterior Nasal Septum

Current companion diagnostics in advanced colorectal cancer; getting a bigger and better piece of the pie

Is Liquid-Based Cytology the Magic Bullet for Performing Molecular Techniques?

Contact Info

Product

Resources

About