KPC-2 producing Pseudomonas putida as an unexpected pathogen of catheter-associated bloodstream infection

Chamon, Raiane Cardoso; Rocha, Jaqueline; Martins, Isabella Araujo; Pires, Laís Lopes; Almeida, Breno Macêdo de; Leite, Nahara Souza; Souza, Cláudia Rezende Vieira de Mendonça; Zahner, Viviane; Ribeiro, Rachel Leite; Chagas, Thiago Pavoni Gomes; Martins, Ianick Souto

doi:10.3855/jidc.12145

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…This illustrates the demand for clear etiological discrimination in patients with fever. P. putida is widely found on hospital surfaces and moist environments and, as an opportunistic pathogen, can cause bloodstream infections [ 26 , 27 ]. Therefore, bloodstream infection surveillance is essential to characterize the public health threat [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostics and next-generation sequencing reveal real etiological characteristics of invasive Salmonella infection in febrile illness in Freetown, Sierra Leone

Zhao

Lü

Tie

et al. 2022

Emerging Microbes & Infections

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Invasive Salmonella infection, which can cause typhoid/paratyphoid fever and invasive non-typhoidal salmonellosis, is a public health burden in Africa. Accurate diagnosis and etiological characterization are required to conduct prevalence and risk estimations for Salmonella infection; however, the utilization of optimal techniques and surveillance data are still insufficient. In this study, we performed a laboratory-based survey in Freetown, which is the biggest city in Sierra Leone with a high burden of typhoid fever, by using blood culture and molecular methods but not the Widal test, to estimate the prevalence and aetiology of invasive Salmonella infection among fever patients. We found a very low prevalence of typhoid fever in patients with fever during the investigation period, and this prevalence was clearly overestimated by the Widal test. Genome sequencing of the S . Typhi isolate from this work revealed that the strain carried multiple antibiotic resistance genes, and an epidemic clone that has existed in West Africa for years was also detected in Sierra Leone. By using metagenomic sequencing, one patient with invasive non-typhoidal salmonellosis was identified as having bacterial co-infections. Our data highlight that Salmonella surveillance based on accurate laboratory diagnosis and genome sequencing needs to be strengthened to provide a better estimation of the real epidemics and enable potential risk assessment by etiological analysis in Africa. Even in a laboratory with only basic equipment, it is possible to conduct next-generation sequencing for pathogen discovery in bloodstream infections and to determine the etiological characteristics of pathogene without complex combinations of laboratory methods.

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Section: Discussionmentioning

confidence: 99%

Molecular diagnostics and next-generation sequencing reveal real etiological characteristics of invasive Salmonella infection in febrile illness in Freetown, Sierra Leone

Zhao

Lü

Tie

et al. 2022

Emerging Microbes & Infections

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show abstract

“…Infections by other Pseudomonas species with an environmental origin and biotechnological potential, as Pseudomonas putida [ 112 , 113 , 114 , 115 ], have also been reported, although their prevalence is much lower. Besides intrinsic resistance determinants [ 116 , 117 ], the acquisition of carbapenemases, as KPC-2 [ 113 ], constitutes an additional risk for the efficient treatment of infections by these pathogens.…”

Section: Pseudomonas Aeruginosamentioning

confidence: 99%

Coming from the Wild: Multidrug Resistant Opportunistic Pathogens Presenting a Primary, Not Human-Linked, Environmental Habitat

Sanz-García

Gil-Gil

Laborda

et al. 2021

IJMS

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The use and misuse of antibiotics have made antibiotic-resistant bacteria widespread nowadays, constituting one of the most relevant challenges for human health at present. Among these bacteria, opportunistic pathogens with an environmental, non-clinical, primary habitat stand as an increasing matter of concern at hospitals. These organisms usually present low susceptibility to antibiotics currently used for therapy. They are also proficient in acquiring increased resistance levels, a situation that limits the therapeutic options for treating the infections they cause. In this article, we analyse the most predominant opportunistic pathogens with an environmental origin, focusing on the mechanisms of antibiotic resistance they present. Further, we discuss the functions, beyond antibiotic resistance, that these determinants may have in the natural ecosystems that these bacteria usually colonize. Given the capacity of these organisms for colonizing different habitats, from clinical settings to natural environments, and for infecting different hosts, from plants to humans, deciphering their population structure, their mechanisms of resistance and the role that these mechanisms may play in natural ecosystems is of relevance for understanding the dissemination of antibiotic resistance under a One-Health point of view.

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“…Although, Pseudomonas aeruginosa is one of the principal agent responsible for hospital acquired infection [ 109 , 110 ], the other species of the genus (hereinafter called Pseudomonas sp. non aeruginosa ) exhibit limited pathogenicity to humans [ 111 ]. Pseudomonas AMR is mainly studied for P. aeruginosa , where acquired resistance to carbapenems could occur i) by mutation onto genes conducting to overexpression of efflux system or to overexpression of the cephalosporinase AmpC or to modification of porin protein D2 and ii) by HGT [ 110 ].…”

Section: The Bacterial Shuttle Concept: When Hydric Autochtonous Bmentioning

confidence: 99%

“…Resistance by carbapenemase production in Pseudomonas non aeruginosa is less described in clinical reports. However, the carriage of CEG like bla KPC-2 and bla VIM-2 (carried on an class 1 integron) respectively in a clinical isolates of Pseudomonas putida and Pseudomonas fulva has been noticed [ 111 , 112 ].…”

Section: The Bacterial Shuttle Concept: When Hydric Autochtonous Bmentioning

confidence: 99%

The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

2020

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Carbapenems are β-lactams antimicrobials presenting a broad activity spectrum and are considered as last-resort antibiotic. Since the 2000s, carbapenemase producing Enterobacterales (CPE) have emerged and are been quickly globally spreading. The global dissemination of carbapenemase encoding genes (CEG) within clinical relevant bacteria is attributed in part to its location onto mobile genetic elements. During the last decade, carbapenemase producing bacteria have been isolated from non-human sources including the aquatic environment. Aquatic ecosystems are particularly impacted by anthropic activities, which conduce to a bidirectional exchange between aquatic environments and human beings and therefore the aquatic environment may constitute a hub for CPE and CEG. More recently, the isolation of autochtonous aquatic bacteria carrying acquired CEG have been reported and suggest that CEG exchange by horizontal gene transfer occurred between allochtonous and autochtonous bacteria. Hence, aquatic environment plays a central role in persistence, dissemination and emergence of CEG both within environmental ecosystem and human beings, and deserves to be studied with particular attention.

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KPC-2 producing Pseudomonas putida as an unexpected pathogen of catheter-associated bloodstream infection

Cited by 7 publications

References 10 publications

Molecular diagnostics and next-generation sequencing reveal real etiological characteristics of invasive Salmonella infection in febrile illness in Freetown, Sierra Leone

Molecular diagnostics and next-generation sequencing reveal real etiological characteristics of invasive Salmonella infection in febrile illness in Freetown, Sierra Leone

Coming from the Wild: Multidrug Resistant Opportunistic Pathogens Presenting a Primary, Not Human-Linked, Environmental Habitat

The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

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