KP4 to control Ustilago tritici in wheat: Enhanced greenhouse resistance to loose smut and changes in transcript abundance of pathogen related genes in infected KP4 plants

Quijano, Carolina Diaz; Wichmann, Fabienne; Schlaich, Thomas; Fammartino, Alessandro; Huckauf, Jana; Schmidt, Kerstin; Unger, Christoph; Broer, Inge; Sautter, Christof

doi:10.1016/j.btre.2016.08.002

Cited by 17 publications

(6 citation statements)

References 29 publications

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“…Stinking or smut is one of the most important seed-borne diseases of wheat . One of the wheat pathogens responsible from the Ustilaginales is Tilletia caries , which causes the stinking smut, and Ustilago tritici causes loose smut . When the seeds are germinated, the latent hyphae also begin to germinate, and with the growth and development of wheat, the disease spreads upward through the growth point and invades the ear of wheat .…”

Section: Introductionmentioning

confidence: 99%

Enantioselective Fungicidal Activity and Toxicity to Early Wheat Growth of the Chiral Pesticide Triticonazole

Liu

Zhang

Deng

et al. 2021

J. Agric. Food Chem.

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In this study, the enantioselective activity of the chiral fungicide triticonazole (TRZ) against target pathogens of seedborne diseases and the effect on the early phenotypic indexes and physiological and biochemical indexes of wheat following the soaking of seeds were studied. S-TRZ had low fungicidal activity and significantly inhibited the germination of wheat seeds. Seedlings in the S-TRZ treatment group suffered severe oxidative damage; the contents of glutathione (GSH) and malondialdehyde (MDA) were significantly increased. S-TRZ significantly inhibited the synthesis of gibberellin (GA) and ethylene (ETH), while it significantly increased that of jasmonic acid (JA). R-TRZ showed the highest activity against two smut fungi; the treatment of seeds with 5 g (ai)/100 kg R-TRZ had little influence on early wheat growth. The plant hormones were synthesized normally; seedlings grew well, and the fresh weight increased significantly following this treatment. Based on these data, we propose to develop and apply R-TRZ using the national recommended doses to control seed-borne diseases of wheat.

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Section: Introductionmentioning

confidence: 99%

Enantioselective Fungicidal Activity and Toxicity to Early Wheat Growth of the Chiral Pesticide Triticonazole

Liu

Zhang

Deng

et al. 2021

J. Agric. Food Chem.

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“…Roussel tritici Jensen (UST), is one of the most serious diseases on wheat ( Triticum aestivum L.) globally. The disease is favored by moist and cool climate during anthesis (Quijano et al, 2016). This fungus converts the spike floral tissues to fungal teliospores, causing yield losses equivalent to the percent smutted spikes (Green et al, 1968; Singh, 2018).…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and Population Structure Analysis Reveal Diversity by Gene Flow and Mutation in Ustilago segetum (Pers.) Roussel tritici Causing Loose Smut of Wheat

et al. 2019

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Ustilago segetum (Pers.) Roussel tritici (UST) causes loose smut of wheat account for considerable grain yield losses globally. For effective management, knowledge of its genetic variability and population structure is a prerequisite. In this study, UST isolates sampled from four different wheat growing zones of India were analyzed using the second largest subunit of the RNA polymerase II ( RPB2 ) and a set of sixteen neutral simple sequence repeats (SSRs) markers. Among the 112 UST isolates genotyped, 98 haplotypes were identified. All the isolates were categorized into two groups ( K = 2), each consisting of isolates from different sampling sites, on the basis of unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure. The positive and significant index of association (I A = 1.169) and standardized index of association (rBar D = 0.075) indicate population is of non-random mating type. Analysis of molecular variance showed that the highest variance component is among isolates (91%), with significantly low genetic differentiation variation among regions (8%) (F st = 0.012). Recombination (R m = 0) was not detected. The results showed that UST isolates have a clonal genetic structure with limited genetic differentiation and human arbitrated gene flow and mutations are the prime evolutionary processes determining its genetic structure. These findings will be helpful in devising management strategy especially for selection and breeding of resistant wheat cultivars.

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“…Loose smut of wheat caused by the basidiomycete fungus Ustilago tritici , is one of the most serious diseases on wheat ( Triticum aestivum L.) globally. The disease is favored by moist and cool climate during anthesis (Quijano et al, 2016). This fungus converts the spike floral tissues to fungal teliospores, and the carrier seeds of loose smut are the only way of transmission (Kumar et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The yield losses of a single plant are nearly 100% after the onset of disease, and the general disease rate is 1–5%. When the incidence is serious, it is more than 10%, which can reduce the yield of wheat by 5–20% (Quijano et al, 2016). With the popularization of wheat planting area, the damage caused by loose smut in wheat has become more serious, and it has gradually become one of the most harmful diseases in wheat cultivation (Knox, Menzies & Sharma, 2012).…”

Section: Introductionmentioning

confidence: 99%

qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici

Yan¹,

Zhang²,

Ma³

et al. 2019

PeerJ

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Loose smut of wheat caused by the basidiomycete fungus Ustilago tritici, a seed-borne disease, is difficult to control because of the expanse of wheat planting area and difficulty in pathogen detection. In this study, real-time fluorescence quantitative PCR (qPCR) and loop-mediated isothermal amplification (LAMP) assays are used to rapidly amplify the DNA of U. tritici. Five pairs of primers for qPCR and two series primers for LAMP were designed. Primarily, the specificity of the primer was assessed by using genomic DNA of U. tritici, Fusarium graminearum, Blumeria graminis, Rhizoctonia cerealis, Puccinia striiformis, Bipolaris sorokiniana, and Alternaria solani as templates. Further, the amplification systems were optimized. Finally, the sensitivity of qPCR and LAMP assays were evaluated. The results showed that the primer Y-430 F/R, Y-307 F/R, Y-755 F/R, and Y-139 F/R for qPCR and primers L-139 and L-988 for LAMP could be used for U. tritici detection. In the sensitivity test, the detection limit of qPCR assay was identified as 10 pg μL−1 of genomic DNA, the detection limit for LAMP assay was 100 fg μL−1. We successfully performed qPCR and LAMP assays on wheat loose smut wheat samples. This paper establishes two methods for U. tritici detection, which can be used for diagnosis of wheat loose smut in the laboratory and in the field.

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KP4 to control Ustilago tritici in wheat: Enhanced greenhouse resistance to loose smut and changes in transcript abundance of pathogen related genes in infected KP4 plants

Cited by 17 publications

References 29 publications

Enantioselective Fungicidal Activity and Toxicity to Early Wheat Growth of the Chiral Pesticide Triticonazole

Enantioselective Fungicidal Activity and Toxicity to Early Wheat Growth of the Chiral Pesticide Triticonazole

Phylogeography and Population Structure Analysis Reveal Diversity by Gene Flow and Mutation in Ustilago segetum (Pers.) Roussel tritici Causing Loose Smut of Wheat

qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici

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