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Mechanical Properties of Injection Molded B4C–C Ceramics

“…Consequently 2 wt% carbon black was selected as the safe lower limit of carbon additions in the present work. To prepare homogeneous B C-C batches, 100 parts of the B C powder were mixed with 2/3/4 and 5 parts of carbon black, this being achieved by wet-dispersing of the powders in deionized water (8). After spray drying of the wet-sieved (20 m mesh size) slurries, the granulated powders were further processed to shaped components via injection molding.…”

ATP hydrolysis by the SNF2 domain of the ultraspecific maintenance methylase Dnmt5 drives recognition and modification of hemimethylated DNA

“…The sensitivity of ATPase activity to the CG motif raised the possibility that the SNF2 domain is coupled to hemimethylated CG detection by the DNMT domain, a protein fold able to make multiple contacts to a CG motif and to undergo conformational rearrangements when its preferred substrate is present (Matje et al, 2011;Song et al, 2012). To test this model, we sought a DNA substrate that would bind the DNMT domain and stabilize it in such a conformation.…”

“…To test this model, we sought a DNA substrate that would bind the DNMT domain and stabilize it in such a conformation. Extensive study of cytosine methylation by the M.HhaI methyltransferase has elucidated a precatalytic pathway in which the enzyme 1) binds its DNA substrate, 2) destabilizes the target cytosine, causing it to flip out of the DNA helix, and 3) undergoes a conformational rearrangement to close its catalytic loop, creating a 'closed' active site capable of methylation (Matje et al, 2011;Roberts and Cheng, 1998;Sankpal and Rao, 2002).…”

“…Interestingly, replacing the target cytosine with an abasic site (while preserving the phosphate backbone and ribose ring) increases M.HhaI DNMT domain affinity for the substrate while stabilizing the 'closed' catalytic loop conformation (Matje et al, 2011;O'Gara et al, 1998).…”