Korrelation zwischen mikrobiellem Wachstum in Bindehautabstrichen von Hornhautspendern und Kontamination des Organkulturmediums

Li, S.; Bischoff, Markus; Schirra, F.; Langenbucher, Achim; Ong, Michelle; Halfmann, Alexander; Herrmann, Mathias; Seitz, Berthold

doi:10.1007/s00347-013-2901-3

Cited by 14 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our extensive decontamination protocol, as described above, could explain why our organ culture media contamination rate of 1.0% was lower than the rates of 4.5% to 10.8% found by Li et al, [ 27 ] who cleaned the periocular region with a 7.5% povidone-iodine solution spray and the ocular surface with a 1.25% povidone-iodine solution for 3 and 5 minutes, respectively. Furthermore Li et al abstained from the use of gentamicin, and they prepared the corneoscleral disc directly at the donor site instead of in a class II biological safety cabinet, without decontaminating twice, as in our study.…”

Section: Discussionmentioning

confidence: 77%

See 1 more Smart Citation

Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes

et al. 2018

View full text Add to dashboard Cite

This study assessed the influence of donor, environmental, and logistical factors on the contamination rates of the conjunctival swabs and organ culture media of human donor eyes.In total, 1008 conjunctival swabs and 418 organ culture media samples from 504 consecutive human donor eyes were analyzed. Cross-tabulation, chi-squared tests, and Fisher's exact tests were used to assess the influence of the different factors on the contamination rates of the conjunctival swabs and organ culture media.The overall contamination rates were 28.4% for the conjunctival swabs and 1.0% for the organ culture media. A prolonged time between death and the conjunctival swab collection was associated with an increased conjunctival swab contamination rate [odds ratio (OR) = 1.9, 95% confidence interval (CI) = 1.2–3.0, P = .007]. The highest conjunctival swab contamination rate was found in the corneas procured in external institutions (outside the university hospital) (44.1%, OR = 3.6, 95%CI = 1.5–8.4, P = .003). Hospitalization times of 2 to 7 days prior to death were associated with an increased conjunctival swab contamination risk (OR = 2.6, 95%CI = 1.1–5.8, P = .021). However, the sex, age, cause of donor death, differentiation between septic and aseptic donors, differentiation between heart-beating brain-dead multiorgan donors and cadaveric donors, a warmer mean monthly temperature, and death to corneoscleral disc excision time did not significantly increase the conjunctival swab contamination risk. In addition, none of these factors affected the organ culture media contamination risk. Moreover, a positive conjunctival swab did not significantly increase the media contamination risk (P = .08). Surprisingly, the microorganisms causing media contamination were present at 50% of the amount detected on the conjunctival surface of the respective donor eye.A prolonged time between death and the conjunctival swab collection, a hospitalization time of 2 to 7 days prior to death, and corneal collection outside the university hospital seemed to be the main factors responsible for an increased conjunctival swab contamination risk. In addition, our investigation illustrated that a positive conjunctival swab is not a strong indicator for organ culture media contamination. Critical discussion is necessary regarding the validity of conjunctival swabs as prognostic parameters for organ culture media contamination.

show abstract

Section: Discussionmentioning

confidence: 77%

“…[ 22 , 23 ] In spite of donor eye decontamination with povidone-iodine, persistent microorganismal colonization has been demonstrated. [ 24 – 27 ]…”

Section: Introductionmentioning

confidence: 99%

Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, in total, we decontaminated the donor eyes for at least 8 min in low concentrations of povidone-iodine and transported the eyes separately in cooled sterile urine cups filled with 5 ml of gentamicin eye drops between the 2 decontaminations. Our extensive decontamination protocol, as described above, could explain why our organ culture media contamination rate of 1.0% was lower than the rates of 4.5% to 10.8% found by Li et al, who cleaned the periocular region with a 7.5% povidone-iodine solution spray and the ocular surface with a 1.25% povidone-iodine solution for 3 and 5 min, respectively [11,17].…”

Section: Discussionmentioning

confidence: 97%

“…Up until January 2018, the German guidelines on the collection and processing of donor corneas have required 2 microbiological examinations of the donor’s cornea using conjunctival swabs after disinfection and before the corneal excision or enucleation [7,8]. Despite donor eye decontamination with povidone-iodine, persistent microorganismal colonization has been demonstrated [9–11]. Without decontamination, different authors describe a conjunctival swab contamination rate of around 90% in cadaver donors [12–16].…”

Section: Introductionmentioning

confidence: 99%

Donor-Dependent Factors Influencing Contamination Rates of Conjunctival Swabs of Human Donor Eyes

et al. 2019

View full text Add to dashboard Cite

Background This study investigated the effect of donor-dependent factors on contamination rates of conjunctival swabs of human donor eyes. Material/Methods From July 2015 to September 2017 a total of 1008 conjunctival swabs from 504 consecutive human donor eyes were analyzed. Cross-tabulation, chi-squared tests, and Fisher’s exact tests were used to evaluate the effect of donor-dependent factors on contamination rates of conjunctival swabs. Results The mean conjunctival swabs contamination rate was 28.4%. Donors with the diagnosis of carcinoma or metastases were associated with an increased conjunctival swab contamination rate [odds ratio (OR)=1.8, 95% confidence interval (CI)=1.2–2.6, p=0.007; OR=1.7, 95% CI=1.1–2.6, p=0.016; respectively]. However, the age, sex, diagnosis of diabetes mellitus, and donors who received chemotherapy did not significantly increase the conjunctival swab contamination risk. Conclusions Donors with the diagnosis of a carcinoma or metastases seemed to be predisposed to increased conjunctival swab contamination risk.

show abstract

“…Hornhäute werden hier in der Regel bei einer Temperatur von 31-37°C in Kultur gehalten. Als Medium wird meist "Minimum Essential Medium"(MEM)-Earle's mit Zusatz von fötalem bovinem Serum (FBS) und Antibiotika/Antimykotika verwendet [7]. Die v. a. in den USA verbreitete sog.…”

Section: Hornhautspende Im Rahmen Der Covid-19-pandemieunclassified

Bedeutung der Hornhautorgankultur bei Spendern mit möglicher SARS-CoV-2-Infektion

et al. 2020

View full text Add to dashboard Cite

show abstract

Korrelation zwischen mikrobiellem Wachstum in Bindehautabstrichen von Hornhautspendern und Kontamination des Organkulturmediums

Cited by 14 publications

References 20 publications

Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes

Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes

Donor-Dependent Factors Influencing Contamination Rates of Conjunctival Swabs of Human Donor Eyes

Bedeutung der Hornhautorgankultur bei Spendern mit möglicher SARS-CoV-2-Infektion

Contact Info

Product

Resources

About