Konzentration an Deoxynivalenol (DON) in Thüringer Brot und Feinen Backwaren

Abstract

Since 2003 bread and fine bakery products must not contain more than 350 μg Deoxynivalenol (DON). As a prerequisite for the label "Geprüfte Qualität"-Thüringen [proved quality Thuringia] and since coming into force of the respective evaluation prescription a total of 25 breads and 41 fine bakery products was investigated by ELISA with regard of this compound. In the 66 samples of the year 2003 no DON concentrations above the limit were detected and in the preparation period 2001/2002 only one sample exceeded the maximum. In several samples, most narrow to the maximum limit, an additional determination by HPLC was performed which resulted in lower DON-concentrations as compared to the values analyzed by ELISA.

Stable isotope dilution analysis of the Fusarium mycotoxins deoxynivalenol and 3‐acetyldeoxynivalenol

“…(iii) ELISA tests are useful for sample screening, but produce higher results, which are often not reproducible with chromatographic methods. This is probably due to cross reactions with structural analogues [8,9]. (iv) GC methods in general require laborious sample preparation and a derivatization step prior to analysis.…”