Konsentrasi Efektif Ekstrak Daun Salam (Syzygium polyanthum Wight) terhadap Hambatan Biofilm Enterococcus faecalis  (Effective Concentration of Bay Leaf Extract (Syzygium polyanthum Wight) to Inhibit Enterococcus faecalis Biofilm)

Aini, Shufiyah Nurul; Effendy, Ruslan; Widjiastuti, Ira

doi:10.20473/cdj.v6i2.2016.87-92

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Materials commonly used for conventional root canal irrigation have weaknesses because they are resistant to enterococci bacteria, thus requiring natural materials that can be developed as alternative materials for root canal irrigation that are less toxic, more biocompatible, and easily available. Natural materials that can be utilized for root canal irrigation are aloe vera extracts and chitosan shrimp shells [14, 16,17]. Aloe vera is becoming increasingly popular due to its high concentration of tannins, polyphenols, amino acids, anthraquinones, minerals, vitamins, and salicylic acid.…”

Section: Introductionmentioning

confidence: 99%

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2022

J. Med. Chem. Sci.

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Enterococcus faecalis is a resistant bacterium in the root canal that can cause root canal treatment failure and is frequently found in root canal infections due to its ability to form biofilm. As a natural ingredient, aloe vera extract and chitosan can be used as an antibiofilm. This study aims to compare the antibiofilm properties of aloe vera extract and chitosan shrimp shells. The antibiofilm test was divided into two groups: groups I and II, tested 24 h and 72 h after biofilm formation by adding 0.1 mL of aloe vera suspension and 0.1 mL of chitosan suspension in 96 well plates containing E. faecalis. The aloe vera extract and chitosan concentrations used were 50%, 25%, 12.5%, and 6.25%. 0.1% crystal violet staining and OD values were measured using an ELISA reader. In the first group, aloe vera extracts at 25% concentration were more significant on positive control and had a percentage of biofilm inhibition of 93.77%, whereas chitosan at 25% concentration was most significant on positive control and had a percentage of biofilm inhibition of 90.59%. In the second group, aloe vera extracts of 12.5% concentration were most significant on positive control and had a percentage of biofilm inhibition of 90.68%, while chitosan at 25% concentration was most significant on positive control and had a percentage of biofilm inhibition of 88.37%. Aloe vera extract at a concentration of 12.5% can inhibit the growth of Enterococcus faecalis biofilms more effectively compared with that of the shrimp shell chitosan.

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Section: Introductionmentioning

confidence: 99%

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2022

J. Med. Chem. Sci.

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Antibacterial and Antibiofilm Activity of Ethanol Extract of Batak Onion Bulbs (Allium chinense G.Don.) against Streptococcus mutans and Enterococcus faecalis.

Octiara,

Sipangkar,

Prist

2024

Biomed. Pharmacol. J.

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Background: S.mutans is the main pathogen causing caries, while E.faecalis is the dominant microorganism in dental root canals. Batak onion is one of Indonesia's biological resources frequently used as condiments by Batak tribes and possesses antibacterial compounds such as alkaloids, saponins, and flavonoids. Aims: To analyze antibacterial activity test based on Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and antibiofilm from 70% ethanol extract of Batak onion bulbs against S.mutans and E.faecalis. Material and Methods: This type of research is a laboratory experiment with a post-test only control group design. Determination of MIC by Kirby Baurer disc diffusion method, determination of MBC by streaking method, and colony tests are calculated using colony counters, determination of antibiofilm by Static Microtiter Plate Assays method, and checking the optical density at around 600nm. Data analysis of MIC, MBC, and antibiofilm using Oneway Anova and Post Hoc LSD tests. Results: MIC on S.mutans and E.faecalis was at a concentration of 0.78% with an average of one of inhibition of 9.00 ± 0.43 mm and 8.06 ± 0.20 mm; no MBC was found because no group was able to reduce bacteria 98-98%, Batak onion bulb extract has antibiofilm ability starting at a concentration of 0.78% with the ability to reduce S.mutans bacteria by 84.55% and 85.73% on E.faecalis. Conclusion: 70% ethanol extract of Batak onion bulbs can inhibit bacterial growth and biofilm of S.mutans and E.faecalis. The recommended dose for antibacterial and antibiofilm is a 6.25-50% concentration.

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Konsentrasi Efektif Ekstrak Daun Salam (Syzygium polyanthum Wight) terhadap Hambatan Biofilm Enterococcus faecalis (Effective Concentration of Bay Leaf Extract (Syzygium polyanthum Wight) to Inhibit Enterococcus faecalis Biofilm)

Cited by 2 publications

References 12 publications

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Antibacterial and Antibiofilm Activity of Ethanol Extract of Batak Onion Bulbs (Allium chinense G.Don.) against Streptococcus mutans and Enterococcus faecalis.

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