Konjac glucomannan defends against high-fat diet-induced atherosclerosis in rabbits by promoting the PI3K/Akt pathway

Weng, Junting; Chen, Min; Shi, Bingbing; Liu, Danjuan; Weng, Shuoyun; Guo, Rongjie

doi:10.1016/j.heliyon.2023.e13682

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…However, the expression of these genes was significantly downregulated in 12 m mice compared to the control group (Table A3). The PI3K-AKT signalling pathway serves an important role in regulating cell proliferation, differentiation, apoptosis, and senescence [41]. Previous studies have found that inhibition of the PI3K-AKT signalling pathway can induce cell senescence, and directly increase the expression of p21 [42,43].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss

Feng,

Zhou,

et al. 2023

Biomolecules

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Age-related hearing loss (ARHL), also known as presbycusis, is one of the most common neurodegenerative disorders in elderly individuals and has a prevalence of approximately 70–80% among individuals aged 65 and older. As ARHL is an intricate and multifactorial disease, the exact pathogenesis of ARHL is not fully understood. There is evidence that transcriptional dysregulation mediated by epigenetic modifications is widespread in ARHL. However, the potential role of N6-methyladenosine (m6A) modification, as a crucial component of epigenetics, in ARHL progression remains unclear. In this study, we confirmed that the downregulation of m6A modification in cochlear tissues is related to ARHL and found that the expression of the m6A methylation regulators Wilms tumour suppressor-1-associated protein (WTAP), methyltransferase-like 3 (METTL3), ALKB homologous protein 5 (ALKBH5) and fat mass and obesity-associated protein (FTO) is decreased significantly at the mRNA and protein levels in ARHL mice. Then, we used methylated RNA immunoprecipitation sequencing (MeRIP-Seq) and RNA sequencing (RNA-Seq) to identify the differentially m6A-methylated genes in the cochlear tissues of ARHL mice. A total of 3438 genes with differential m6A methylation were identified, of which 1332 genes were m6A-hypermethylated and 2106 genes were m6A-hypomethylated in the ARHL group compared to the control group according to MeRIP-seq. Further joint analysis of RNA-Seq and MeRIP-Seq data showed that 262 genes had significant differences in both mRNA expression and m6A methylation. GO and KEGG analyses indicated that 262 unique genes were enriched mainly in the PI3K-AKT signalling pathway. In conclusion, the results of this study reveal differential m6A methylation patterns in the cochlear tissues of ARHL mice, providing a theoretical basis for further study of the pathogenesis of ARHL and potential therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss

Feng,

Zhou,

et al. 2023

Biomolecules

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“…Additionally, glucomannan influences the activation of the PI3K/AKT pathway, which is implicated in developing atherosclerotic plaque [11].…”

Section: Introductionmentioning

confidence: 99%

Glucomannan from Porang (Amorphophallus muelleri) Improves Short-Chain Fatty Acid in Wistar Rat with High-Fat and High-Fructose Diet

Harimawan

2024

Babali Nurs. Res.

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Introduction: Short-chain fatty acids (SCFAs) improve lipid profile and prevent coronary artery disease. Searching for nutrition based on local foods that might raise the body's SCFA levels is imperative. Porang (Amorphophallus muelleri) is a plant with a high concentration of glucomannan that is investigated to have a healthy benefit. This study aimed to investigate the effect of glucomannan from Porang (A.muelleri) on SCFA in Wistar rats with a high-fat, high-fructose diet.Methods: This was an experimental study with a randomized and post-test-only control group design. Thirty male Wistar rats were divided into five groups: a normal control group, a negative control group given a high-fat, high-fructose diet, and treatment groups given Porang glucomannan 25mg, 50mg, or 100mg/200gBW. Twenty-eight days after the intervention, the SCFA level was measured using gas chromatography–masstry (GC/MS). Data were analyzed in SPSS.Results: The treatment group with Porang glucomannan 50 mg/200gBW has the highest mean SCFA level (3.98±0.83 nmol/ml) compared to normal control (1.56±0.24), negative control (2.18±0.45), treatment group 25mg/200gBW (1.81±0.26), and treatment group 100mg/200gBW (2.58±0.38). Kruskal Wallis test showed significant differences among groups (p<0.001). Post hoc test revealed that SCFA in the treatment group 50 mg was significantly higher than the normal control and negative control group.Conclusion: Glucomannan from Porang (A.muelleri) at 50 mg/200gBW doses improves short-chain fatty acid in Wistar rats with high-fat and high-fructose diets.

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Lipid metabolism regulation by dietary polysaccharides with different structural properties

Yin,

Zhong,

Liu

et al. 2024

International Journal of Biological Macromolecules

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Konjac glucomannan defends against high-fat diet-induced atherosclerosis in rabbits by promoting the PI3K/Akt pathway

Cited by 4 publications

References 29 publications

Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss

Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss

Glucomannan from Porang (Amorphophallus muelleri) Improves Short-Chain Fatty Acid in Wistar Rat with High-Fat and High-Fructose Diet

Lipid metabolism regulation by dietary polysaccharides with different structural properties

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