Konfokale Laserscan-Mikroskopie

Astner, Susanne; Ulrich, Martina

doi:10.1007/s00105-009-1878-y

Cited by 22 publications

(10 citation statements)

References 30 publications

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“…While CLSM is well established as helpful non-invasive tool with almost histological resolution in the diagnosis of lesions of the human skin, primarily in pigmented lesions but also in other skin tumours and diseases; [42][43][44][45][46][47][48][49] it can also be helpfully applied for the examination of the nail plate. In a recent study, morphological features of CLSM of the healthy nail plate were described and compared with the typical findings in onychomycosis and leuconychia.…”

Section: Clsmmentioning

confidence: 99%

Confocal laser scanning microscopy as a new valuable tool in the diagnosis of onychomycosis – comparison of six diagnostic methods

Rothmund

Sattler

Kaestle

et al. 2012

Mycoses

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Onychomycosis is common and can mimic several different nail disorders. Accurate diagnosis is essential to choose the optimum antifungal therapy. The aim of this study was to evaluate the use of confocal laser scanning microscopy (CLSM) and optical coherence tomography (OCT) as new non-invasive diagnostic tools in onychomycosis and to compare them with the established techniques. In a prospective trial, 50 patients with suspected onychomycosis and 10 controls were examined by CLSM and OCT. Parallel KOH preparation, culture, PAS-staining and PCR were performed. PCR showed the highest sensitivity, followed by CLSM, PAS and KOH preparation. OCT offered the second best sensitivity but displayed the lowest specificity. CLSM and KOH preparation showed a high specificity and CLSM offered the best positive predictive value, similar to KOH preparation and OCT. Fungal culture showed the lowest sensitivity and the worst negative predictive value, yet culture and PCR are the only techniques able to identify genus and species. In summary, CLSM was comparable to PAS staining and superior to KOH preparation. Due to the low specificity we assess OCT not as appropriate. In the differentiation of species PCR outplays the fungal culture in terms of time and sensitivity.

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Section: Clsmmentioning

confidence: 99%

Confocal laser scanning microscopy as a new valuable tool in the diagnosis of onychomycosis – comparison of six diagnostic methods

Rothmund

Sattler

Kaestle

et al. 2012

Mycoses

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“…By assembling of images from different focus planes, three-dimensional images can be obtained (17). In this small volume, scattering occurs as a result of differences in the refractive index.…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

“…The emitted backscattered laser radiation is collected by a photomultiplier tube (PMT) after having passed a pinhole aperture, thus avoiding signals from planes other than the focus plane. By assembling of images from different focus planes, three-dimensional images can be obtained (17).…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

Clinical application of multiphoton tomography in combination with confocal laser scanning microscopy for in vivo evaluation of skin diseases

Koehler

Speicher

Lange‐Asschenfeldt

et al. 2011

Experimental Dermatology

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Multiphoton tomography (MPT) is an in vivo imaging technique with very high spatial resolution and efforts are made to combine MPT with other non-invasive imaging methods. The goals of the present study were the description of the features of different dermatological entities as seen in MPT and confocal laser scanning microscopy (CLSM) comparison of these two novel techniques and the 'classical' diagnostic measures visual inspection, dermoscopy and histology with respect to the strengths and weaknesses of the different methods and the potential benefit from their combined implementation. After study approval by the local Ethics Committee, 47 patients (31 male, 16 female, age range: 24-88 years) were recruited from the Department of Dermatology of the University Hospital Jena. In this work, we present an illustrative selection of eleven cases from a clinical study combining in vivo MPT with in vivo CLSM. The patients presented with a broad range of dermatological disorders including seborrheic keratoses, angioma, actinic keratoses, melanocytic nevi, malignant melanoma, psoriasis, pemphigus vulgaris and scarring. Both methods, CLSM and MPT, were found to be suitable for in vivo imaging of superficial skin layers and may therefore be useful in dermatological practice for the diagnosis of skin diseases. However, both methods differ in their technical and physical principles. Thus, despite of many similarities concerning the morphological presentation of cells and tissues, important differences are recognized. Synergies of the combination of CLSM and MPT may be obtained by combined implementation in order to benefit from the fast overview given by CLSM and the detailed imaging of skin structures by MPT.

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“…Simultaneous measurements of anodic biofilms during the MFC's operation may be obscured by the need to apply labeling materials. This method is therefore used more commonly as a useful ex situ method [23,24].…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%