Kombinationen enzymhistochemischer Methoden zur Fasertypendifferetzierung und Beurteilung der Skeletmuskulatur

Ziegan, J

doi:10.1016/s0065-1281(79)80030-6

Cited by 36 publications

(15 citation statements)

References 7 publications

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“…Commercially available mouse monoclonal antibodies, raised against rabbit skeletal muscle, were used to identify fast‐twitch (type II) and slow‐twitch (type I) fibres (for detailed protocol, see Schmidt & Schilling, 2007). To cross‐check fibre identification, enzyme histochemistry following Ziegan’s protocol (Ziegan, 1979; modified after von Mering & Fischer, 1999) was additionally performed on a fresh sample from the male chimpanzee (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

Distribution patterns of fibre types in the triceps surae muscle group of chimpanzees and orangutans

2011

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Different locomotor and postural demands are met partly due to the varying properties and proportions of the muscle fibre types within the skeletal muscles. Such data are therefore important in understanding the subtle relationships between morphology, function and behaviour. The triceps surae muscle group is of particular interest when studying our closest living relatives, the non-human great apes, as they lack a significant external Achilles tendon, crucial to running locomotion in humans and other cursorial species. The aim of this study, therefore, was to determine the proportions of type I (slow) and type II (fast) fibres throughout these muscles in chimpanzees and orangutans using immunohistochemistry. The orangutan had a higher proportion of type I fibres in all muscles compared with the chimpanzees, related to their slower, more controlled movements in their arboreal habitat. The higher proportion of type II fibres in the chimpanzees likely reflects a compromise between their need for controlled mobility when arboreal, and greater speed and power when terrestrial. Overall, the proportion of slow fibres was greater in the soleus muscle compared with the gastrocnemius muscles, and there was some evidence of proximal to distal and medial to lateral variations within some muscles. This study has shown that not only do orangutans and chimpanzees have very different muscle fibre populations that reflect their locomotor repertoires, but it also shows how the proportion of fibre types provides an additional mechanism by which the performance of a muscle can be modulated to suit the needs of a species.

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Section: Methodsmentioning

confidence: 99%

Distribution patterns of fibre types in the triceps surae muscle group of chimpanzees and orangutans

2011

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“… Results of the enzyme histochemical reaction. Oxidative fibres (type‐I according to Ziegan 1979) were stained blue, oxidative‐glycolytic (IIa) dark brown and glycolytic fibres (IIb) light brown …”

Section: Methodsmentioning

confidence: 99%

Characteristics of paravertebral muscles - fibre type distribution pattern in the pika, Ochotona rufescens (Mammalia: Lagomorpha)

Schilling

2005

J Zoological System

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The evolution of the locomotor apparatus in vertebrates is marked by major reorganizations in trunk's musculature. The hypothesized functions of mammalian back muscles in the literature are discussed under consideration of the distribution and proportion of oxidative, type-I-fibres, oxidativeglycolytic, type-IIa-fibres and glycolytic, type-IIb-fibres in paravertebral muscles of a small mammal. The fibre type distribution was examined from a complete series of histological sections maintaining topographical relationships between the muscles as well as within the muscle, in order to establish the overall distribution pattern. The deep and short muscles showed the highest percentage of oxidative fibres. The larger, superficial paravertebral muscles contained the highest percentage of glycolytic fibres. Two muscles were intermediate in their proportion of fibre types. All epaxial muscles together can be interpreted as an antigravity muscle-complex counteracting enduringly against the rebound tendency caused by gravitation, comparable with antigravity muscles in limbs. A gradient from deep to superficial, or a clear regionalization of oxidative muscle fibres in central deep regions around a large intramuscular tendon was found in the m. spinalis and the m. quadratus lumborum, respectively. Concepts of the function of human back muscles as those of A. Bergmark (1989: Acta Orthop. Scand. 230, 1) or S.G.T. Gibbons & M.J. Comerford (2001: Orthop. Division Rev.March/April, 21) were exposed to be more general within mammals. Functional specializations of different muscles and muscle parts are discussed under the consideration of evolutionary reorganization of the paravertebral musculature in tetrapods.Along the cranio-caudal axis, the percentage of oxidative fibres was decreased in caudal direction within the same muscles, whereas the proportion of glycolytic fibres was increased. Therefore, classifications of muscles as ÔglycolyticÕ or ÔoxidativeÕ based on biopsies or analyses of single cross-sections may result in wrong interpretations. Changes in the proportions of the fibre type distribution pattern were mostly due to oxidative and glycolytic fibre types, whereas the percentage of oxidative-glycolytic fibres had only minor influence. A significant positive correlation between the cross-sectional area of the single fibre and its percentage in the area investigated were observed for oxidative fibres, whereby the size was positive correlated to the proportion of the oxidative fibres.

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“…Muscle fiber type determination was based on the alkaline combination staining method of Ziegan [1979]. This method combines the myosin ATPase reaction after alkaline preincubation (pH 9.6) and the NADH 2 tetrazolium salt reductase reaction on the same section.…”

Section: Histochemistrymentioning

confidence: 99%

“…Using the NADH 2 tetrazolium reductase and the myosin ATPase procedures according to Ziegan [1979] in succession on the same cross-section, three muscle fiber types could be visualized: type I (M. soleus, blue), type IIA (FOG, golden brown with fine blue network) and type IIB (FG, golden brown).…”

Section: Histochemistrymentioning

confidence: 99%

“…Only slow-twitch and fast-twitch fibers could be distinguished until the 2nd week of age using the alkaline myosin ATPase method [Padykula and Herman, 1955] or alkaline combination method according to Ziegan [1979] for histochemical fiber type determination. Two weeks after birth, in some muscles differentiation of fast-twitch fibers into fibers with low and high oxidative capacity started, but the distinction of type II fibers with predominant oxidative and glycolytic metabolism, respectively, was not possible by the alkaline combination method before 4 weeks after birth.…”

Section: Fiber Type Distribution During Postnatal Developmentmentioning

confidence: 99%

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Muscle Growth and Fiber Type Composition in Hind Limb Muscles during Postnatal Development in Pigs

Wank

Fischer

Walter

et al. 2006

Cells Tissues Organs

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Rapid postnatal development in pigs is reflected by differentiation in skeletal muscle. This process depends on muscle function and demands, but a comprehensive overview of individual developmental characteristics of quickly growing leg muscles in pigs is still missing. This study focused on the development of 10 hind limb muscles in pigs. To determine these changes in mass, fiber type patterns and fiber diameters were analyzed 0, 2, 4, 7, 14, 28, 42, 56 and 400 days after birth. Generally, the proportion of slow fibers increased from birth to 8 weeks. Thereafter, only minor changes in muscle fiber type composition were observed. The majority of the muscles contained less then 10% slow-twitch fibers at birth, increasing to between 12 (Musculus vastus lateralis) and 38% (M. gastrocnemius medialis) in adult pigs. By contrast, postural muscles already had 20–30% slow fibers at birth, and this contribution increased up to 65% in adults (i.e. M. vastus intermedius). From birth to the 2nd week, only in slow fibers could activity of oxidative enzymes be detected. A differentiation of fast-twitch fibers into subtypes with high (comparable to type IIA) and low oxidative metabolism (equivalent to type IIB) occurred between the 2nd and 4th week of life. The ratio between type II fibers with high and low oxidative enzyme activity did not change markedly through development in any muscle, although there was a trend towards an increasing proportion of type IIA fibers in the soleus. In the majority of the muscles investigated, the fast-twitch fibers with low oxidative metabolism (IIB) obtained the largest cross-sectional area. In contrast, at birth no remarkable differences in the diameter of fast and slow fibers were found. The rapid increase in muscle mass compared to body mass reflects the high performance in meat production of the cross pig investigated.

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Kombinationen enzymhistochemischer Methoden zur Fasertypendifferetzierung und Beurteilung der Skeletmuskulatur

Cited by 36 publications

References 7 publications

Distribution patterns of fibre types in the triceps surae muscle group of chimpanzees and orangutans

Distribution patterns of fibre types in the triceps surae muscle group of chimpanzees and orangutans

Characteristics of paravertebral muscles - fibre type distribution pattern in the pika, Ochotona rufescens (Mammalia: Lagomorpha)

Muscle Growth and Fiber Type Composition in Hind Limb Muscles during Postnatal Development in Pigs

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