Kolaviron attenuates diclofenac-induced nephrotoxicity in male Wistar rats

Abstract

The beneficial effects of kolaviron, a natural biflavonoid from the seeds of Garcinia kola, have been attributed to its antioxidant and anti-inflammatory activities. This study was designed to investigate the renoprotective effect of kolaviron in rat model of diclofenac (DFC)-induced acute renal failure. Thirty-five male Wistar rats were divided into 7 groups of 5 rats each as follows: a control group that received propylene glycol orally and treatment groups that received DFC, DFC recovery, DFC followed by kolaviron at 3 different doses, and kolaviron only. DFC-treated rats showed sluggishness, illness, and anorexia. Their urine contained appreciable protein, glucose, and ketone bodies. Histopathological examination of their kidneys revealed profound acute tubular necrosis. DFC treatment significantly increased levels of plasma creatinine, urea, sodium, chloride, potassium ions, and increased renal tissue activities of superoxide dismutase, catalase, levels of malondialdehyde, and hydrogen peroxide. Fractional excretion of sodium and potassium and renal tissue levels of reduced glutathione and prostaglandin E (PGE) decreased significantly in DFC-treated groups. However, kolaviron administration significantly reduced the toxic effect of DFC on PGE release; plasma levels of creatinine, urea, glucose, and electrolytes; and significantly attenuated renal tubular and oxidative damages. Furthermore, the effects of DFC administration on food consumption, water intake, urine output and urine protein, glucose, ketone bodies, and electrolytes were significantly attenuated in animals treated with kolaviron. The results suggested that kolaviron ameliorated DFC-induced kidney injury in Wistar rats by decreasing renal oxidative damage and restoration of renal PGE release back to the basal levels.

Betterment of Diclofenac-Induced Nephrotoxicity by Pentoxifylline Through Modulation of Inflammatory Biomarkers

“…The present study illustrated significant nephrotoxicity which induced by diclofenac through elevation of blood urea and serum creatinine compared to the control in the experimental rats as supported by Alabi et al [18].…”

Kolaviron Diminishes Diclofenac-Induced Liver and Kidney Toxicity in Wistar Rats Via Suppressing Inflammatory Events, Upregulating Antioxidant Defenses, and Improving Hematological Indices

“…These results are in accordance with the previous researches. 17,26,37 Ateşşahín et al 38 and Abdel-Daim et al 39 have confirmed ROS over generation in kidney and liver tissues as a result of increase in MDA concentrations in the renal and hepatic tissues, which resulted to necrosis and back leak of liver enzymes and kidney function makers into circulation. Interestingly, the protective effect observed for KV in both the kidneys and liver of rats treated with DF indicated that KV may be involved in the elimination of ROS or other reactive bye products generated by DF toxic metabolites in both the kidney and liver tissues.…”

“…Kolaviron was isolated from Garcinia kola according to the previous methods. 24,26 The KV obtained was dissolved in propylene glycol (0.2 mL/administration) and given to rat according to the designed dosage.…”

Synergistic effect of berberine and pentoxifylline in attenuation of acute kidney injury

“…The present study demonstrated noteworthy AKI that was induced by diclofenac through the rise of blood urea and serum creatinine compared with control in the experimental rats as maintained by a previous study that showed diclofenac leads to significant renal tubular necrosis and AKI. [16]…”