Koi herpesvirus disease

Chong, Roger Sie-Maen

doi:10.1016/b978-0-12-812211-2.00014-7

Cited by 1 publication

(1 citation statement)

References 27 publications

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“…However, this approach has a limited application in KHV diagnosis. The KF-1 cell line was developed to isolate KHV; however, cell culture is time-consuming and overall it is less sensitive than PCR for KHV detection [ 100 ]. On the whole, KHV can be isolated in only a limited number of cell lines, which can be difficult to handle.…”

Section: Herpesviralesmentioning

confidence: 99%

Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution

Volpe

Errani

Mandrioli

et al. 2023

Biology

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Aquaculture is the fastest-growing food-producing sector, with a global production of 122.6 million tonnes in 2020. Nonetheless, aquatic animal production can be hampered by the occurrence of viral diseases. Furthermore, intensive farming conditions and an increasing number of reared fish species have boosted the number of aquatic animals’ pathogens that researchers have to deal with, requiring the quick development of new detection and study methods for novel unknown pathogens. In this respect, the molecular tools have significantly contributed to investigating thoroughly the structural constituents of fish viruses and providing efficient detection methods. For instance, next-generation sequencing has been crucial in reassignment to the correct taxonomic family, the sturgeon nucleo-cytoplasmic large DNA viruses, a group of viruses historically known, but mistakenly considered as iridoviruses. Further methods such as in situ hybridisation allowed objectifying the role played by the pathogen in the determinism of disease, as the cyprinid herpesvirus 2, ostreid herpesvirus 1 and betanodaviruses. Often, a combination of molecular techniques is crucial to understanding the viral role, especially when the virus is detected in a new aquatic animal species. With this paper, the authors would critically revise the scientific literature, dealing with the molecular techniques employed hitherto to study the most relevant finfish and shellfish viral pathogens.

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Section: Herpesviralesmentioning

confidence: 99%