Kohlrabi-Based Amperometric Biosensor for Hydrogen Peroxide Measurement

Chen, Lu; Lin, Min-Sheng; Hara, Minoru; Rechnitz, G. A.

doi:10.1080/00032719108052879

Cited by 40 publications

(13 citation statements)

References 7 publications

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“…Table 4 summarizes the publications on tissue-, cell-, and organellc-modified carbon pastes. Examples of such are banana (polyphenol oxidase) [38,203,2041 [92,2001, zucchini (ascorbate oxidase) [98], pineapple (peroxidase) [206], eggplant (polyphenol oxidasej [207], kohlrabi (peroxidase) [208], tomato seed (alcohol dehydrogenase) [209], pea seedlings (diamine oxidase) [176], papaya (papain) [210], spinach (cinnamic acid hydrolase) [211], Hansenuh anomalu (cytochrome h2) [202], spinach (catechol oxidase) [212], oat seedlings (polyamine oxidase, peroxidase) [2 131, Gluconohacter industrius (glucose PQQdehydrogenase) [2 141, spinach (glycolate oxidase, peroxidase) [215] modified-carbon pastes. These tissue-or cellbased modified electrodes have been mostly used for sensing a variety of substrates.…”

Section: Tissue-and Cell-modified Carbon Pastes and Composite Electrodesmentioning

confidence: 99%

Carbon paste electrodes modified with enzymes, tissues, and cells

1995

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A review is presented dealing with the use of carbon paste amperometric electrodes for electroanalytical purposes, with either the surface or the bulk being modified with biologically derived material such as enzymes, tissues, and cells. It covers virtually all the publications which have appeared from the very first enzyme-modified carbon paste electrode up to early 1994 and includes 220 references.

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Section: Tissue-and Cell-modified Carbon Pastes and Composite Electrodesmentioning

confidence: 99%

Carbon paste electrodes modified with enzymes, tissues, and cells

1995

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“…Diversos procedimentos são citados na literatura para determinação de peróxido de hidrogênio usando diferentes tecidos vegetais, como aspargo, uva, abacaxi, rabanete, nabo, alface, entre outros e extrato bruto de abobrinha, como fonte da enzima peroxidase 40,47,48,[155][156][157] .…”

Section: Peróxido De Hidrogêniounclassified

Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Fatibello‐Filho¹,

Vieira²

2002

Quím. Nova

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“…Tissues of several plants such as asparagus, 38,39 grape, 40 pineapple, 41 kohlrabi, 42 tobacco callus, 43 horseradish roots, 44,45 turnip 46 and sweet potato 47 have been used as POx sources. Vieira and Fatibello-Filho 48 have chosen the crude extract of zucchini as a POx source of very high specific activity and have used it for the spectrophotometric determination of H2O2.…”

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confidence: 99%

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

2006

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Crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared by a simple procedure and its enzymatic activity and total protein concentration were determined. It was found that this crude extract is a rich source of peroxidase (POx) and has high specific activity. Cross-linked polyvinylpyrrolidone was used as a stabilizer in the preparation of the crude extract. The POx activity of kohlrabi crude extract did not vary for at least 2 months when deoxygenated and stored at 4˚C. This extract was applied for the spectrofluorometric determination of hydrogen peroxide using homovanillic acid as a fluorogenic substrate. POx catalyzes the hydrogen peroxide oxidation of homovanillic acid to produce a dimer which shows strong fluorescence at 420 nm with excitation at 312 nm. In the optimum conditions, the calibration graph for hydrogen peroxide was linear up to 190 ng mL -1 , with a detection limit of 4.4 ng mL -1 . The relative standard deviation (RSD) was 1.48% for 50 ng mL -1 hydrogen peroxide. The proposed method was successfully applied to the determination of hydrogen peroxide in honey. The concentration-time profile of H2O2 produced upon dilution of honey was studied and H2O2 contents of some different honeys from various areas of Iran were determined.

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Kohlrabi-Based Amperometric Biosensor for Hydrogen Peroxide Measurement

Cited by 40 publications

References 7 publications

Carbon paste electrodes modified with enzymes, tissues, and cells

Carbon paste electrodes modified with enzymes, tissues, and cells

Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

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