Kochia scoparia induces apoptosis of oral cancer cells in vitro and in heterotopic tumors

Han, Hogyu; Lee, Haeng-Eun; Kim, Hyung Joon; Jeong, Seung-Hwa; Kim, Jung-Hoon; Kim, Hyungwoo; Ryu, Mi Heon

doi:10.1016/j.jep.2016.09.019

Cited by 16 publications

(13 citation statements)

References 24 publications

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“…Han et al. (2014, 2016) showed that methanol extract of K. scoparia fruit at a dose of 25–40 µg/mL could inhibit growth of breast and oral cancer cells through caspase-dependent apoptosis. This suppression of cancer cell proliferation is in agreement with our data that KSE induced apoptotic cell death in prostate cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…1997; Han et al. 2016). Previous studies reported that K. scoparia fruit contains abundant saponins (Xia et al.…”

Section: Introductionmentioning

confidence: 99%

“…2013, 2014) and oral squamous cell carcinoma (Han et al. 2016). Although K. scoparia has shown promising cancer prevention activity, whether or not K. scoparia can modulate angiogenesis and proliferation of prostate cancer has not been determined.…”

Section: Introductionmentioning

confidence: 99%

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Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

Cho

Kim

Park

et al. 2019

Pharmaceutical Biology

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Context: Kochia scoparia (L.) Schrad (Amaranthaceae), known as a traditional medicine in China, Japan and Korea, is reported to have various biological activities. However, K. scoparia seed extract (KSE) functional roles on angiogenesis and prostate cancer inhibition have not been elucidated.Objective: This study elucidates the effects of KSE on vascular endothelial growth factor (VEGF)-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) and inhibition of proliferation in prostate cancer cells.Materials and methods: HUVECs were treated with 10–20 µg/mL of KSE and 20–50 ng/mL of VEGF for 12–72 h. Anti-angiogenesis properties of KSE were determined by wound healing, trans-well, tube formation, rat aortic ring assay and western blotting. Prostate cancer and normal cells were incubated with 10–250 µg/mL of KSE for 24 h, and cell viability was measured by SRB assay. Phenolic compounds in KSE were analyzed using a HPLC-PDA system.Results: IC50 for cell viability of HUVECs, LNCaP, PC-3, RC-58T and RWPE-1 by KSE were 30.64, 89.25, 123.41, 141.62 and >250 µg/mL, respectively. Treatment with KSE (20 µg/mL) significantly suppressed VEGF-induced migration, invasion and capillary-like structure formation of HUVECs and microvessel sprouting from rat aortic rings. In addition, KSE down-regulated PI3K/AKT/mTOR levels and phosphorylation of VEGF receptor 2 in HUVECs. 3-OH-tyrosol (1.63 mg/g) and morin hydrate (0.17 mg/g) were identified in KSE.Conclusions: KSE inhibits angiogenesis in HUVECs as well as proliferation in human prostate cancer cells, suggesting KSE may be useful herbal medicine for preventing progression of prostate cancer and angiogenesis.

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Section: Discussionmentioning

confidence: 99%

“…1997; Han et al. 2016). Previous studies reported that K. scoparia fruit contains abundant saponins (Xia et al.…”

Section: Introductionmentioning

confidence: 99%

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Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

Cho

Kim

Park

et al. 2019

Pharmaceutical Biology

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“…Hey Yeon Han et al showed that the methanol extracted from the dried fruit of K. scoparia (MEKS) inhibits cell proliferation and induces apoptosis in MDA-MB-231 breast cancer cell line [6]. They also showed the apoptotic effect of essential oil of K. scoparia fruit on oral squamous cell carcinoma (OSCC) cell line using the MTT method [7]. In this study, the possible cytotoxic and anticancer effects of K. scoparia and red clover on the MCF-7 breast cancer cell line were investigated.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor Effects of Essential Oils of Red Clover and Ragweed on MCF-7 Breast Cancer Cell Line

Kianinodeh

Tabatabaei

Alibakhshi

et al. 2017

mcij

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Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bassia scoparia on MCF-7 cells. Methods: Essential oils were extracted from Bassia scoparia and red clover plants, by a Clevenger condenser and mass spectrometry was performed for qualitative analysis, then MCF-7 and HU02 cells were treated with different concentrations of these essential oils at different time intervals. The viability of cells was measured by the MTT assay. The results were analyzed using the one-way ANOVA and Tukey′s test. Results: Essential oils of Kochia scoparia and red clover have cytotoxic effects on MCF7 cells. The MTT analysis showed decreased cell viability percentage in treated cells (P < 0.05). In addition, the microscopic examination of cells treated with essential oils revealed morphological changes of apoptotic cells in MCF-7 cells. Conclusions: The findings of this study show that essential oils of Bassia scoparia and Red clover have toxic and anticancer effects on MCF-7 cells.

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“…It grows commonly in China, Japan, and Korea, and its mature fruit has been used throughout the area in traditional medicine to treat diseases including skin problems and inflammatory and allergic diseases [18]. Rosa multiflora is a species of rose native to Eastern Asia, such as China, Japan, and Korea.…”

Section: Introductionmentioning

confidence: 99%

A Mixture of Extracts of Kochia scoparia and Rosa multiflora with PPAR α/γ Dual Agonistic Effects Prevents Photoaging in Hairless Mice

Jeon

Kim

Nho³

et al. 2016

IJMS

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Activation of peroxisome proliferator-activated receptors (PPAR) α/γ is known to inhibit the increases in matrix metalloproteinase (MMP) and reactive oxygen species (ROS) induced by ultraviolet light (UV). Extracts of natural herbs, such as Kochia scoparia and Rosa multiflora, have a PPAR α/γ dual agonistic effect. Therefore, we investigated whether and how they have an antiaging effect on photoaging skin. Eighteen-week-old hairless mice were irradiated with UVA 14 J/cm2 and UVB 40 mJ/cm2 three times a week for 8 weeks. A mixture of extracts of Kochia scoparia and Rosa multiflora (KR) was topically applied on the dorsal skin of photoaging mice twice a day for 8 weeks. Tesaglitazar, a known PPAR α/γ agonist, and vehicle (propylene glycol:ethanol = 7:3, v/v) were applied as positive and negative controls, respectively. Dermal effects (including dermal thickness, collagen density, dermal expression of procollagen 1 and collagenase 13) and epidermal effects (including skin barrier function, epidermal proliferation, epidermal differentiation, and epidermal cytokines) were measured and compared. In photoaging murine skin, KR resulted in a significant recovery of dermal thickness as well as dermal fibroblasts, although it did not change dermal collagen density. KR increased the expression of dermal transforming growth factor (TGF)-β. The dermal effects of KR were explained by an increase in procollagen 1 expression, induced by TGF-β, and a decrease in MMP-13 expression. KR did not affect basal transepidermal water loss (TEWL) or stratum corneum (SC) integrity, but did decrease SC hydration. It also did not affect epidermal proliferation or epidermal differentiation. KR decreased the expression of epidermal interleukin (IL)-1α. Collectively, KR showed possible utility as a therapeutic agent for photoaging skin, with few epidermal side effects such as epidermal hyperplasia or poor differentiation.

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Kochia scoparia induces apoptosis of oral cancer cells in vitro and in heterotopic tumors

Cited by 16 publications

References 24 publications

Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

Anti-tumor Effects of Essential Oils of Red Clover and Ragweed on MCF-7 Breast Cancer Cell Line

A Mixture of Extracts of Kochia scoparia and Rosa multiflora with PPAR α/γ Dual Agonistic Effects Prevents Photoaging in Hairless Mice

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