Knufia obscura sp. nov. and Knufia victoriae sp. nov., two new species from extreme environments

Isola, Daniela; Prigione, Valeria; Zucconi, Laura; Varese, Giovanna Cristina; Poli, A.; Turchetti, Benedetta; Canini, Fabiana

doi:10.1099/ijsem.0.005530

Cited by 6 publications

(11 citation statements)

References 45 publications

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“…A systematic review of available literature for Knu a species suggests that the morphological features of conidiophores and conidia observed in the strain FJI-L2-BK-P2 have not been noticed or reported in other members of Knu a members, whereas similar condiophores and conidia formations were prominently described in several members of Phialocephala, a genus of the order Helotiales 29 . Some other morphological characteristics seen in FJI-L2-BK-P2 have been observed in other Knu a species 25 . Chlamydospore-like propagules were noticed in K. hypolithi in terminal and intercalary positions.…”

Section: Discussionsupporting

confidence: 67%

“…During the early phase of our investigation in 2021, the ITS sequence of FJI-L2-BK-P2 did not align with any known fungal species. However, in 2022, K. obscura was described by molecular phylogeny and published as a new species 25 . This development delayed our own publication and prompted us to request gDNA of the K. obscura type strain to generate WGS and ensure a con dent phylogenetic a liation for FJI-L2-BK-P2.…”

Section: Resultsmentioning

confidence: 99%

“…The objective of this study was to characterize the strain Knu a obscura FJI-L2-BK-P2, which was isolated from the SAF used for the Mars 2020 mission at the National Aeronautics and Space Administration's (NASA) Jet Propulsion Laboratory (JPL). The morphological features of strain FJI-L2-BK-P2 were compared to those of the type strain of Knu a obscura CBS 148926 T that was isolated from a car's gasoline tank in Italy 25 . The whole genome sequence (WGS) for both strains of K. obscura were generated, assembled, annotated, and subsequently used for multi-locus sequence analysis (MLSA) and WGS phylogeny.…”

Section: Introductionmentioning

confidence: 99%

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Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Chander,

Teixeira,

Singh

et al. 2023

Preprint

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Members of the family Trichomeriaceae, belonging to the Chaetothyriales order and the Ascomycota phylum, are known for their capability to inhabit hostile environments characterized by extreme temperatures, nutrient scarcity (oligotrophic conditions), presence of toxic compounds, or drought conditions. Within this family, the genus Knufia encompasses the vast majority of polyextremophilic species. In this report, the genomic and morphological features of the strain FJI-L2-BK-P2, identified as Knufia obscura, are presented. The identification is based on multi-locus sequence analysis and whole genome sequence phylogeny. The strain FJI-L2-BK-P2 was isolated from the Mars 2020 mission spacecraft assembly facility located at the Jet Propulsion Laboratory in Pasadena, California. Additionally, the type strain of K. obscura (CBS 148926T), originally isolated from a car's gas tank in Italy, has been sequenced and annotated. The morphological analysis and description of the genomic characteristics of K. obscura FJI-L2-BK-P2 may contribute to refining the taxonomy of Knufia species. Key morphological features are reported in this K. obscura strain, resembling microsclerotia and chlamydospore-like propagules. These features, known to be significant in black fungi, could potentially facilitate their adaptation to harsh environments.

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Section: Discussionsupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Chander,

Teixeira,

Singh

et al. 2023

Preprint

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“…The objective of this study was to characterize the strain FJI-L2-BK-P2, which was isolated from the SAF used for the Mars 2020 mission at the National Aeronautics and Space Administration's (NASA) Jet Propulsion Laboratory (JPL). The morphological features of strain FJI-L2-BK-P2 were compared to those of the type strain of Knufia obscura CBS 148926 T that was isolated from a the gasoline tank of a car in Italy 26 . The whole genome sequences (WGS) for both strains of K. obscura were generated, assembled, annotated, and subsequently used for multi-locus sequence analysis (MLSA) and WGS phylogeny.…”

Section: Introductionmentioning

confidence: 99%

Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Chander,

de Melo Teixeira,

Singh

et al. 2024

Sci Rep

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Members of the family Trichomeriaceae, belonging to the Chaetothyriales order and the Ascomycota phylum, are known for their capability to inhabit hostile environments characterized by extreme temperatures, oligotrophic conditions, drought, or presence of toxic compounds. The genus Knufia encompasses many polyextremophilic species. In this report, the genomic and morphological features of the strain FJI-L2-BK-P2 presented, which was isolated from the Mars 2020 mission spacecraft assembly facility located at the Jet Propulsion Laboratory in Pasadena, California. The identification is based on sequence alignment for marker genes, multi-locus sequence analysis, and whole genome sequence phylogeny. The morphological features were studied using a diverse range of microscopic techniques (bright field, phase contrast, differential interference contrast and scanning electron microscopy). The phylogenetic marker genes of the strain FJI-L2-BK-P2 exhibited highest similarities with type strain of Knufia obscura (CBS 148926T) that was isolated from the gas tank of a car in Italy. To validate the species identity, whole genomes of both strains (FJI-L2-BK-P2 and CBS 148926T) were sequenced, annotated, and strain FJI-L2-BK-P2 was confirmed as K. obscura. The morphological analysis and description of the genomic characteristics of K. obscura FJI-L2-BK-P2 may contribute to refining the taxonomy of Knufia species. Key morphological features are reported in this K. obscura strain, resembling microsclerotia and chlamydospore-like propagules. These features known to be characteristic features in black fungi which could potentially facilitate their adaptation to harsh environments.

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“…Notes — Knufia includes melanised ascomycetes that are commonly found in extreme environments, ranging from rock-inhabiting, lichenicolous fungi, opportunistic human pathogens, insect associates and plant pathogens ( Isola et al 2022 ). Knufia aloeicola is related to ‘ Phialocephala’ fluminis and Knufia hypolithi ( Crous et al 2021a ), but is phylogenetically and morphologically distinct.…”

mentioning

confidence: 99%

Fungal Planet description sheets: 1478–1549

Crous¹,

Osieck²,

Shivas³

et al. 2023

persoonia

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Novel species of fungi described in this study include those from various countries as follows: Australia, Aschersonia mackerrasiae on whitefly, Cladosporium corticola on bark of Melaleuca quinquenervia, Penicillium nudgee from soil under Melaleuca quinquenervia, Pseudocercospora blackwoodiae on leaf spot of Persoonia falcata, and Pseudocercospora dalyelliae on leaf spot of Senna alata. Bolivia, Aspicilia lutzoniana on fully submersed siliceous schist in high-mountain streams, and Niesslia parviseta on the lower part and apothecial discs of Erioderma barbellatum on a twig. Brazil, Cyathus bonsai on decaying wood, Geastrum albofibrosum from moist soil with leaf litter, Laetiporus pratigiensis on a trunk of a living unknown hardwood tree species, and Scytalidium synnematicum on dead twigs of unidentified plant. Bulgaria, Amanita abscondita on sandy soil in a plantation of Quercus suber. Canada, Penicillium acericola on dead bark of Acer saccharum, and Penicillium corticola on dead bark of Acer saccharum. China, Colletotrichum qingyuanense on fruit lesion of Capsicum annuum. Denmark, Helminthosphaeria leptospora on corticioid Neohypochnicium cremicolor. Ecuador (Galapagos), Phaeosphaeria scalesiae on Scalesia sp. Finland, Inocybe jacobssonii on calcareous soils in dry forests and park habitats. France, Cortinarius rufomyrrheus on sandy soil under Pinus pinaster, and Periconia neominutissima on leaves of Poaceae. India, Coprinopsis fragilis on decaying bark of logs, Filoboletus keralensis on unidentified woody substrate, Penicillium sankaranii from soil, Physisporinus tamilnaduensis on the trunk of Azadirachta indica, and Poronia nagaraholensis on elephant dung. Iran, Neosetophoma fici on infected leaves of Ficus elastica. Israel, Cnidariophoma eilatica (incl. Cnidariophoma gen. nov.) from Stylophora pistillata. Italy, Lyophyllum obscurum on acidic soil. Namibia, Aureobasidium faidherbiae on dead leaf of Faidherbia albida, and Aureobasidium welwitschiae on dead leaves of Welwitschia mirabilis. Netherlands, Gaeumannomycella caricigena on dead culms of Carex elongata, Houtenomyces caricicola (incl. Houtenomyces gen. nov.) on culms of Carex disticha, Neodacampia ulmea (incl. Neodacampia gen. nov.) on branch of Ulmus laevis, Niesslia phragmiticola on dead standing culms of Phragmites australis, Pseudopyricularia caricicola on culms of Carex disticha, and Rhodoveronaea nieuwwulvenica on dead bamboo sticks. Norway, Arrhenia similis half-buried and moss-covered pieces of rotting wood in grass-grown path. Pakistan, Mallocybe ahmadii on soil. Poland, Beskidomyces laricis (incl. Beskidomyces gen. nov.) from resin of Larix decidua ssp. polonica, Lapidomyces epipinicola from sooty mould community on Pinus nigra, and Leptographium granulatum from a gallery of Dendroctonus micans on Picea abies. Portugal, Geoglossum azoricum on mossy areas of laurel forest areas planted with Cryptomeria japonica, and Lunasporangiospora lusitanica from a biofilm covering a biodeteriorated limestone wall. Qatar, Alternaria halotolerans from hypersaline sea water, and Alternaria qatarensis from water sample collected from hypersaline lagoon. South Africa, Alfaria thamnochorti on culm of Thamnochortus fraternus, Knufia aloeicola on Aloe gariepensis, Muriseptatomyces restionacearum (incl. Muriseptatomyces gen. nov.) on culms of Restionaceae, Neocladosporium arctotis on nest of cases of bag worm moths (Lepidoptera, Psychidae) on Arctotis auriculata, Neodevriesia scadoxi on leaves of Scadoxus puniceus, Paraloratospora schoenoplecti on stems of Schoenoplectus lacustris, Tulasnella epidendrea from the roots of Epidendrum × obrienianum, and Xenoidriella cinnamomi (incl. Xenoidriella gen. nov.) on leaf of Cinnamomum camphora. South Korea, Lemonniera fraxinea on decaying leaves of Fraxinus sp. from pond. Spain, Atheniella lauri on the bark of fallen trees of Laurus nobilis, Halocryptovalsa endophytica from surface-sterilised, asymptomatic roots of Salicornia patula, Inocybe amygdaliolens on soil in mixed forest, Inocybe pityusarum on calcareous soil in mixed forest, Inocybe roseobulbipes on acidic soils, Neonectria borealis from roots of Vitis berlandieri × Vitis rupestris, Sympoventuria eucalyptorum on leaves of Eucalyptus sp., and Tuber conchae from soil. Sweden, Inocybe bidumensis on calcareous soil. Thailand, Cordyceps sandindaengensis on Lepidoptera pupa, buried in soil, Ophiocordyceps kuchinaraiensis on Coleoptera larva, buried in soil, and Samsoniella winandae on Lepidoptera pupa, buried in soil. Taiwan region (China), Neophaeosphaeria livistonae on dead leaf of Livistona rotundifolia. Türkiye, Melanogaster anatolicus on clay loamy soils. UK, Basingstokeomyces allii (incl. Basingstokeomyces gen. nov.) on leaves of Allium schoenoprasum. Ukraine, Xenosphaeropsis corni on recently dead stem of Cornus alba. USA, Nothotrichosporon aquaticum (incl. Nothotrichosporon gen. nov.) from water, and Periconia philadelphiana from swab of coil surface. Morphological and culture characteristics for these new taxa are supported by DNA barcodes.

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Knufia obscura sp. nov. and Knufia victoriae sp. nov., two new species from extreme environments

Cited by 6 publications

References 45 publications

Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Fungal Planet description sheets: 1478–1549

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