KnowVolution of a Fungal Laccase toward Alkaline pH

Novoa, Catalina; Dhoke, Gaurao V.; Maté, Diana M.; Martínez, Ronny; Haarmann, Thomas; Schreiter, Martina; Eidner, Jasmin; Schwerdtfeger, Ruth; Lorenz, Patrick; Davari, Mehdi D.; Jakob, Felix; Schwaneberg, Ulrich

doi:10.1002/cbic.201800807

Cited by 47 publications

(38 citation statements)

References 81 publications

(82 reference statements)

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“…Accordingly, laccases have to be identified or engineered that have a shifted optimum pH towards an alkaline pH. One recent successful approach at AB Enzymes has been the engineering of a laccase from the ascomycete Melanocarpus albomyces using a directed evolution approach, which has led to threefold improved kinetics at pH 9.8 [76]. Notably, the principle applicability of laccase-functionalized lignin has been shown, but immediate commercial implementation is hindered by the general availability and inexpensive cost of fossil-based plasticizers.…”

Section: Filamentous Fungi To Mitigate Plastic Pollutionmentioning

confidence: 99%

Growing a circular economy with fungal biotechnology: a white paper

Meyer

Basenko

Benz

et al. 2020

Fungal Biol Biotechnol

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Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum-based economy into a bio-based circular economy and has the ability to sustainably produce resilient sources of food, feed, chemicals, fuels, textiles, and materials for construction, automotive and transportation industries, for furniture and beyond. Fungal biotechnology offers solutions for securing, stabilizing and enhancing the food supply for a growing human population, while simultaneously lowering greenhouse gas emissions. Fungal biotechnology has, thus, the potential to make a significant contribution to climate change mitigation and meeting the United Nation's sustainable development goals through the rational improvement of new and established fungal cell factories. The White Paper presented here is the result of the 2nd Think Tank meeting held by the EUROFUNG consortium in Berlin in October 2019. This paper highlights discussions on current opportunities and research challenges in fungal biotechnology and aims to inform scientists, educators, the general public, industrial stakeholders and policymakers about the current fungal biotech revolution.

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Section: Filamentous Fungi To Mitigate Plastic Pollutionmentioning

confidence: 99%

Growing a circular economy with fungal biotechnology: a white paper

Meyer

Basenko

Benz

et al. 2020

Fungal Biol Biotechnol

Self Cite

287

220

View full text Add to dashboard Cite

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“…The CoV of rM4 without thermal incubation was 13.5 % (Figure S5). CoVs below 20 % have routinely been used in protein engineering campaigns [38–40] …”

Section: Methodsmentioning

confidence: 99%

Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Zhi

Maté

Nöth

et al. 2020

Chemistry A European J

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Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSrtA yielded the cyclized variant CyM6 that has a 7.5 °C increased melting temperature and up to 4.6‐fold increased resistance towards denaturants when compared to the parent rM4. CyM6 gained up to 2.6‐fold (vs. parent rM4) yield of conjugate in ligation of peptide and primary amine under denaturing conditions.

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“…With the same method, an evolved Myceliophthora thermophila laccase was obtained and applied at the synthesis of C-N heteropolymeric dyes at pH 9 [67]. A knowledge-gaining directed evolution approach was employed for the design of a Melanocarpus albomyces laccase mutant [68], bearing two amino acid substitutions close to the T1 copper-binding site. The mutant was shown to have a threefold improved turnover number at pH 9.8.…”

Section: Laccase Production and Improvementmentioning

confidence: 99%

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

et al. 2019

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There is a high number of well characterized, commercially available laccases with different redox potentials and low substrate specificity, which in turn makes them attractive for a vast array of biotechnological applications. Laccases operate as batteries, storing electrons from individual substrate oxidation reactions to reduce molecular oxygen, releasing water as the only by-product. Due to society’s increasing environmental awareness and the global intensification of bio-based economies, the biotechnological industry is also expanding. Enzymes such as laccases are seen as a better alternative for use in the wood, paper, textile, and food industries, and they are being applied as biocatalysts, biosensors, and biofuel cells. Almost 140 years from the first description of laccase, industrial implementations of these enzymes still remain scarce in comparison to their potential, which is mostly due to high production costs and the limited control of the enzymatic reaction side product(s). This review summarizes the laccase applications in the last decade, focusing on the published patents during this period.

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KnowVolution of a Fungal Laccase toward Alkaline pH

Abstract: Scheme1.Schematicdiagram of laccase catalysis. Generalrepresentation of laccase-catalyzedredox cycles for substrate oxidation, with 2,6-dimethoxyphenol (2,6-DMP) as am odel substrate (SUB).

Cited by 47 publications

References 81 publications

Growing a circular economy with fungal biotechnology: a white paper

Growing a circular economy with fungal biotechnology: a white paper

Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

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