Background: Child abuse is a serious problem causing physical, social and psychosocial harm to the children. Aim: To evaluate the effect of educational protocol in improving mothers' knowledge, practice and attitude about child abuse. Design: A quasi-experimental research design [pretest-posttest] was used. Setting: At the out patient's clinics at Zagazig University Hospitals. Sample: A purposive sample consisted of 100 mothers participated in this study and they were chosen according to inclusion criteria. Tools: Four tools were used; Interview questionnaire sheet, Mother's knowledge about child abuse, Child Abuse Prevention Attitude Scale, Check List for Expressed Practices on Child Abuse. Results: Revealed that most of the mothers were secondary education, house wives and from rural areas, there were statistical significant differences between pre\posttests regarding knowledge, practice, attitudes for the mothers regarding child abuse and there was negative association among the incidence of child abuse and the level of knowledge, practice, and attitude of their mothers. Conclusion: The applying of educational protocol for mothers was effective in enhancing their knowledge, practice, and attitudes regarding child abuse. Recommendation: Educational pamphlets about child abuse and its prevention strategies should be given to mothers in different settings such as, clinics, worksites and health care centers, and also establishing child abuse prevention programs at schools could be helpful.