This cross-sectional study aimed to evaluate the knowledge of 316 nurses in a Malaysian public hospital regarding blood transfusion procedures. Convenience sampling was used to select nurses involved in patient care. Data collection employed a validated questionnaire with 33 questions across four sections. Descriptive analysis using SPSS revealed knowledge gaps, particularly in transfusion and adverse reaction management. Findings showed 56.3% with good knowledge, 42.7% with moderate knowledge, and 0.9% with insufficient knowledge. The study emphasizes the importance of targeted education and continuous training to enhance nurses' transfusion-related knowledge and ensure safe practices. Limitations include scope and self-reported data.