Knowledge plays a critical role in organizational resources that enables organizations to gain a competitive advantage. In the today world, the organizations need to investigate new solutions to remain ahead of the competition. Therefore, organizations endeavor to face the challenges by using technologies to enable an efficient management of the e-collaboration and knowledge management. Many models and techniques have been discussed over years for e-collaboration and knowledge management within organizations. Current changes in Information, and Communication Technology (ICT) have prompted organizations to utilize platforms such as corporate portals for collaborative knowledge sharing. This paper introduces an overview of Knowledge Management (KM) and e-collaboration for the enterprise to gain advantage. The paper is appended by a case study of an organization where it applies KM and e-collaboration to cover the business needs and to improve management of enterprise content with collaboration of knowledge. The organizational structures and processes, standards and values are still the main areas that limit the effectiveness of e-collaboration. This requires changing the organizational focus and culture that remains a challenge for many organizations.