Recent advancement in high-resolution confocal imaging has provided valuable novel insights into structural relationships within cells and tissues in vitro and in vivo. Development in volume rendering technique enables visualization of 3D dataset in real-time. Here, we present a system in which 3D models are reconstructed from the stacked 2D images obtained with a confocal microscope, and subsequently visualized using volume rendering technique. Using the reconstructed 3D model, we apply virtual reality method to provide an intuitive way to view and manipulate the 3D cellular environment.