Knowledge and research prospects in marine mollusc and crustacean immunology

Bachère, Evelyne; Mialhe, Eric; Noël, D.; Boulo, Viviane; Morvan, Annie; Rodrı́guez, Jenny

doi:10.1016/0044-8486(94)00389-6

Cited by 160 publications

(65 citation statements)

References 80 publications

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“…Since hemocytes are very abundant in gill tissues, the phenoloxidase activity measured in vitro may be partly due to the hemocytes. Nevertheless, our results are consistent with previous studies using phenoloxidase activity as a marker to measure the immune response of mollusc to external contaminants (Bachère et al 1995;Hellio et al 2007).…”

Section: Discussionsupporting

confidence: 83%

“…Since the phenoloxidase enzyme is strongly involved in immune response, the assessment of its activity is frequently used to evaluate the cell response to external contaminants (Bachère et al 1995;Cerenius et al 2008;Hellio et al 2007). Phenoloxidase activity assays were performed in 96-well microplates.…”

Section: Phenoloxidase Activitymentioning

confidence: 99%

“…Although there is currently no cell lineage in marine invertebrates, primary cell cultures provide suitable tools for complementary in vivo studies. Invertebrate immune defence is mainly mediated by circulating hemocytes (Smith 1991), including phagocytosis, encapsulation, production of antimicrobial compounds as well as the phenoloxidase cascade that is an important reaction of melanisation involved in immunity (Cheng 1981;Bachère et al 1995;Hooper et al 2007;Travers et al 2008c).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures

Pichon

Cudennec

Huchette³

et al. 2013

Cytotechnology

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The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell-V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi. The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for abalone mortalities and LMG7890 which is a nonpathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells-V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.

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Section: Discussionsupporting

confidence: 83%

Section: Phenoloxidase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures

Pichon

Cudennec

Huchette³

et al. 2013

Cytotechnology

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“…The semigranular haemocytes were reported to be the primary cells involved in the phagocytosis of foreign particles in shrimp (Bachere et al, 1995;Soderhall and Cerenius, 1992). Granular haemocytes were also reported to be capable of phagocytosing foreign material but with less frequency than the smaller ones (Hose and Martin, 1989).…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp

Selvin¹,

Huxley²,

Lipton

2004

Aquaculture

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Shrimp disease management using bioactive marine secondary metabolites (MSMs) was developed as a package of practice for the sustainable shrimp farming. Therefore, the effect of MSMs on the host defense factors of shrimp was evaluated in the present study. Findings indicated that Ulva diet significantly increase the defense factors such as haemogram, agglutination index, phagocytic rate, bacterial clearance and serum bactericidal activity of treated shrimps over the control group. Based on the gut bacterial load, Ulva diet was considered as proactive drug whereas Dendrilla diet was determined as a curative agent. D

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“…Bivalves rely exclusively on the innate immune system (Bachere et al 1995;Canesi et al 2002). Hence, when the physical barriers of shell and epithelia have been breached as a result of injury and/or infection, invading microorganisms, nonself particles, and cell debris are recognized and eliminated by humoral and cellular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Husmann

Abele

Rosenstiel

et al. 2014

Cell Stress and Chaperones

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Increasing temperatures and glacier melting at the Western Antarctic Peninsula (WAP) are causing rapid changes in shallow coastal and shelf systems. Climate change-related rising water temperatures, enhanced ice scouring, as well as coastal sediment runoff, in combination with changing feeding conditions and microbial community composition, will affect all elements of the nearshore benthic ecosystem, a major component of which is the Antarctic soft-shelled clam Laternula elliptica. A 454-based RNA sequencing was carried out on tissues and hemocytes of L. elliptica, resulting in 42,525 contigs, of which 48 % was assigned putative functions. Changes in the expression of putative stress response genes were then investigated in hemocytes and siphon tissue of young and old animals subjected to starvation and injury experiments in order to investigate their response to sedimentation (food dilution and starvation) and iceberg scouring (injury). Analysis of antioxidant defense (Le-SOD and Lecatalase), wound repair (Le-TIMP and Le-chitinase), and stress and immune response (Le-HSP70, Le-actin, and Letheromacin) genes revealed that most transcripts were more clearly affected by injury rather than starvation. The upregulation of these genes was particularly high in the hemocytes of young, fed individuals after acute injury. Only minor changes in expression were detected in young animals under the selected starvation conditions and in older individuals. The stress response of L. elliptica thus depends on the nature of the environmental cue and on age. This has consequences for future population predictions as the environmental changes at the WAP will differentially impact L. elliptica age classes and is bound to alter population structure.

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Knowledge and research prospects in marine mollusc and crustacean immunology

Cited by 160 publications

References 80 publications

Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures

Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures

Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

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