Know your enemy and know yourself – the case of SARS-CoV-2 host factors

Lee, Wai Suet; Yousefi, Meisam; Yan, Biaoguo; Yong, Cythia Lingli; Ooi, Yaw Shin

doi:10.1016/j.coviro.2021.08.007

Cited by 10 publications

(18 citation statements)

References 151 publications

(177 reference statements)

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“…The observed downregulatory phase is well-supported by many studies that have shown that soon after infection, SARS-CoV-2 mRNA transport is stalled and there is a rapid degradation of cytoplasmic mRNA (Kamitami et al, 2006(Kamitami et al, , 2009Huang et al, 2011;Burke et al, 2021;Finkel et al, 2021;Lee et al, 2021). Some believe that the inhibition of nuclear mRNA export is a direct consequence of the widespread mRNA degradation in the cytosol (Burke et al, 2021).…”

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

“…First, nsp1 is a strong inhibitor of translation affecting translation of both host and viral mRNA (Lapointe et al, 2021) by docking its C-terminal domain to the mRNA entry channel of the 40S ribosomal subunit (Schubert et al, 2020; Thoms et al, 2020; Yuan et al, 2020). Second, expression of nsp1 is sufficient to induce the global degradation of host mRNAs (Kamitami et al, 2006, 2009; Huang et al, 2011; Burke et al, 2021; Finkel et al, 2021; Lee et al, 2021) by an unknown mechanism independent of a viral encoded RNase and /or ribonuclease L (Liang et al, 2006; Burke et al, 2021). Third, immunoprecipitation and mass spectrometry studies have shown that the N-terminal domain of nsp1 interacts with the mRNA export receptor protein NFX1 preventing its binding to mRNA export adaptors that results in the accumulation of mRNA in the nucleus (Zhang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Single-cell RNA-sequencing data analysis reveals a highly correlated triphasic transcriptional response to SARS-CoV-2 infection

Sánchez

Elena

2022

Preprint

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Single-cells RNA sequencing (scRNA-seq) is currently one of the most powerful techniques available to study the transcriptional response of cells to external perturbations. However, the use of conventional bulked RNA-seq analysis methods can miss important patterns underlying in the scRNA-seq data. Here, we present a reanalysis of scRNA-seq data from human bronchial epithelial cells and colon and ileum organoids using pseudo-time profiles based on the degree of virus accumulation which reflect the progress of infection. Our analysis revealed a transcriptional response to infection characterized by three distinct up- and down-regulatory phases, that cannot be detected using classical two-group comparisons. Interrogation of results, focused on genes involved in interferon-response, transcription factors and RNA-binding proteins, suggests a highly correlated transcriptional response for most genes. In addition, correlation network analysis revealed a distinct response of genes involved in translation and mitochondrially-encoded genes. Based on our data, we propose a model where modulation of nucleocytoplasmic traffic by the viral protein nsp1 explains the triphasic transcriptional response to SARS-CoV-2 infection.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Single-cell RNA-sequencing data analysis reveals a highly correlated triphasic transcriptional response to SARS-CoV-2 infection

Sánchez

Elena

2022

Preprint

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“…Zotatifin, a selective eIF4A inhibitor that increases the affinity between eIF4A and specific polypurine sequence motifs, has been reported to inhibit translation of driver oncogenes in lymphoma models ( 176 , 202 ). This drug has a strong antiviral effect in case of SARS-CoV-2 infection ( 10 , 193 ). Therefore, inhibition of eEF1A and eIF4A can be extended to other human coronaviruses and beyond unrelated viral pathogens as a strategy for treating viral infections ( 191 ).…”

Section: Translational Strategies Against Sars-cov-2mentioning

confidence: 99%

Translational Control of COVID-19 and Its Therapeutic Implication

et al. 2022

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19, which has broken out worldwide for more than two years. However, due to limited treatment, new cases of infection are still rising. Therefore, there is an urgent need to understand the basic molecular biology of SARS-CoV-2 to control this virus. SARS-CoV-2 replication and spread depend on the recruitment of host ribosomes to translate viral messenger RNA (mRNA). To ensure the translation of their own mRNAs, the SARS-CoV-2 has developed multiple strategies to globally inhibit the translation of host mRNAs and block the cellular innate immune response. This review provides a comprehensive picture of recent advancements in our understanding of the molecular basis and complexity of SARS-CoV-2 protein translation. Specifically, we summarize how this viral infection inhibits host mRNA translation to better utilize translation elements for translation of its own mRNA. Finally, we discuss the potential of translational components as targets for therapeutic interventions.

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“…Endemic diseases like HIV infection, AIDS, Tuberculosis (TB), malaria, and other tropical diseases continue to pose a national threat pre, peri and post coronavirus. All we know is that coronavirus taught us several lessons, the most important of which is to know your opponent [ 20 ]. Diseases, for example, necessitate preparation and a quick response time and identification.…”

Section: Reviewmentioning

confidence: 99%

Comparison of Public Health Investments of Various Countries Amid a Need for Greater Transparency: A Narrative Review

Hasan¹,

Rannaware²,

Choudhari³

2022

Cureus

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A robust health system demands investments in public health and healthcare as they aid in closing the health protection gap. They are primarily responsible for longer life expectancies, disease prevention, and protection. Loopholes in the public health system were formed due to a lack of transparency and have only worsened throughout COVID-19. Spending more on public health is associated with fewer deaths, fewer food-borne illnesses, better sanitation, food safety, clean air and water, increased immunizations to stave against infectious diseases, and a decline in low birth weight. A comprehensive literature and data search was conducted using web-based search engines like PubMed, National Center for Biotechnology Information (NCBI), Google Scholar, Science Direct, and the New England Journal of Medicine. The review study standpoints healthcare spending, out-of-pocket expenditures, and other monetary use in various low-tohigh-income countries, and the results are graphically represented. Countries with a strong public health system provide all the necessary aid to protect their citizens. They have cost-effective, readily available resources with fewer out-of-pocket expenditures (OOPs), government schemes, and health insurance to help their people. During our research, it was found how little the Indian government spends on healthcare as a percentage of gross domestic product (GDP) as compared to 'thought-to-be' poor countries like Bhutan.

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Know your enemy and know yourself – the case of SARS-CoV-2 host factors

Cited by 10 publications

References 151 publications

Single-cell RNA-sequencing data analysis reveals a highly correlated triphasic transcriptional response to SARS-CoV-2 infection

Single-cell RNA-sequencing data analysis reveals a highly correlated triphasic transcriptional response to SARS-CoV-2 infection

Translational Control of COVID-19 and Its Therapeutic Implication

Comparison of Public Health Investments of Various Countries Amid a Need for Greater Transparency: A Narrative Review

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