Know when and how to die: gaining insights into the molecular regulation of leaf senescence

Sasi, Jyothish Madambikattil; Gupta, Shitij; Singh, Alok Kumar; Kujur, Alice; Agarwal, Manu; Katiyar-Agarwal, Surekha

doi:10.1007/s12298-022-01224-1

Cited by 6 publications

(1 citation statement)

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“…After the maturation stage, leaves undergo organ-level senescence, which involves the orderly disassembly of subcellular organelles and macromolecules and the concomitant relocation of hydrolyzed molecules to actively growing organs such as developing seeds (for successful reproduction in annuals) or storage organs such as stems or roots (for the preparation of the next generation in perennials). Thus, despite its degenerative features, leaf senescence is critical for ensuring optimal offspring production and enhancing plant survival ( Lim et al., 2007 ; Sasi et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamic landscape of long noncoding RNAs during leaf aging in Arabidopsis

et al. 2022

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Leaf senescence, the last stage of leaf development, is essential for whole-plant fitness as it marks the relocation of nutrients from senescing leaves to reproductive or other developing organs. Temporally coordinated physiological and functional changes along leaf aging are fine-tuned by a highly regulated genetic program involving multi-layered regulatory mechanisms. Long noncoding RNAs (lncRNAs) are newly emerging as hidden players in many biological processes; however, their contribution to leaf senescence has been largely unknown. Here, we performed comprehensive analyses of RNA-seq data representing all developmental stages of leaves to determine the genome-wide lncRNA landscape along leaf aging. A total of 771 lncRNAs, including 232 unannotated lncRNAs, were identified. Time-course analysis revealed 446 among 771 developmental age-related lncRNAs (AR-lncRNAs). Intriguingly, the expression of AR-lncRNAs was regulated more dynamically in senescing leaves than in growing leaves, revealing the relevant contribution of these lncRNAs to leaf senescence. Further analyses enabled us to infer the function of lncRNAs, based on their interacting miRNA or mRNA partners. We considered functionally diverse lncRNAs including antisense lncRNAs (which regulate overlapping protein-coding genes), competitive endogenous RNAs (ceRNAs; which regulate paired mRNAs using miRNAs as anchors), and mRNA-interacting lncRNAs (which affect the stability of mRNAs). Furthermore, we experimentally validated the senescence regulatory function of three novel AR-lncRNAs including one antisense lncRNA and two mRNA-interacting lncRNAs through molecular and phenotypic analyses. Our study provides a valuable resource of AR-lncRNAs and potential regulatory networks that link the function of coding mRNA and AR-lncRNAs. Together, our results reveal AR-lncRNAs as important elements in the leaf senescence process.

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Section: Introductionmentioning

confidence: 99%