Knockout of the Placenta Specific 8 Gene Affects the Proliferation and Migration of Human Embryonic Kidney 293T Cell

Qin, Xu-Hui; Wang, Haixia; Ma, Li-Qun; Shen, Jinhua; Liu, Qinghua

doi:10.1007/s12013-019-00893-2

Cited by 7 publications

(9 citation statements)

References 36 publications

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“…Cell counting assays were performed to detect the effects of PLAC8 on cell proliferation as previously described with minor modi cation (15,17). In 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St Louis, MO, USA) assay, the cells were seeded at a density of 2,000 cells/well in 96-well tissue culture plates with 200 µL culture medium.…”

Section: Cell Proliferation Analysismentioning

confidence: 99%

Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers

Zhou

et al. 2023

Preprint

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Background As a candidate oncogene, PLAC8 participate in genesis and progression of various tumors. However, the role of PLAC8 in lung cancer (LC) especially non-small cell lung cancer (NSCLC) is still limited. Methods We performed Tissue microarray analysis (TMA) and Real-Time PCR (RT-PCR) to detect the expression levels of PLAC8 in LC tissues and cell lines, respectively. Then a series of cellular experiments focusing on cell proliferation, cell cycle, cell motility were conducted to identified the role of PLAC8 in NSCLC-derived cell lines H1299 and A549. Results TMA and RT-PCR showed that PLAC8 played complicated even opposite roles in different LCs. Further cellular experiments confirmed that PLAC8 could promote cell viability, alter cell cycle, and accelerate cell mobility via regulation of cell cyclins or cadherins, respectively. Conclusions Our study indicated that PLAC8 might participate in LC especially NSCLC progression. Our study also shed new light on the potential role of PLAC8 as a therapeutic biomarker in NSCLC.

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Section: Cell Proliferation Analysismentioning

confidence: 99%

Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers

Zhou

et al. 2023

Preprint

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“…PLAC8 reflects the expression of epithelial-mesenchymal related markers including E-cadherin, N-cadherin and vimentin thus involving epithelialmesenchymal transition process. In breast cancer cells, embryonic kidney 293 T cells, colorectal cancer cells and nasopharyngeal carcinoma cells, PLAC8 downregulates the level of E-cadherin thus regulating cell migration and invasion [34,35,96,97]. On the other hand, PLAC8 upregulates N-cadherin and vimentin levels in breast cancer and nasopharyngeal carcinoma cells [34,73].…”

Section: Epithelial-mesenchymal Transitionmentioning

confidence: 99%

Multifaced roles of PLAC8 in cancer

et al. 2021

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The role of PLAC8 in tumorigenesis has been gradually elucidated with the development of research. Although there are common molecular mechanisms that enforce cell growth, the impact of PLAC8 is varied and can, in some instances, have opposite effects on tumorigenesis. To systematically understand the role of PLAC8 in tumors, the molecular functions of PLAC8 in cancer will be discussed by focusing on how PLAC8 impacts tumorigenesis when it arises within tumor cells and how these roles can change in different stages of cancer progression with the ultimate goal of suppressing PLAC8-relevant cancer behavior and related pathologies. In addition, we highlight the diversity of PLAC8 in different tumors and its functional output beyond cancer cell growth. The comprehension of PLAC8’s molecular function might provide new target and lead to the development of novel anticancer therapies.

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“…Cells were treated with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St Louis, MO, USA) for the cell proliferation assay, as previously described (25,30). Briefly, the cells were seeded at 1,000 cells/well in a 96-well tissue culture plate.…”

Section: Mtt Assay and Colony Formationmentioning

confidence: 99%

“…Wound healing and invasion assays were performed as previously reported (31). 16HBE-GFP-Plac9 or 16HBE-GFP clones (4 × 10 5 ) per well were seeded in a 12-well plate with complete medium (DMEM containing 10% FBS).…”

Section: Wound-healing and Invasion Assaysmentioning

confidence: 99%

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells

et al. 2021

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Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-Plac9). We utilized the proteomic approach isobaric tag for relative and absolute quantification (iTRAQ) to analyze the effect of PLAC9 on cellular protein composition. Gene ontology (GO) and pathway analyses revealed that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility and migration were significantly enriched among the proteins regulated by PLAC9. Our in vitro results showed that PLAC9 overexpression reduced cell proliferation, altered cell cycle progression, and increased cell motility, including migration and invasion. Our findings suggest that PLAC9 inhibits cell proliferation through S phase arrest by altering the expression levels of cyclin/cyclin-dependent kinases (CDKs) and promotes cell motility, likely via the concerted actions of cyclins, E-cadherin, and vimentin. Since these mechanisms may underlie PLAC9-mediated abnormal human bronchial pathogenesis, our study provides a basis for the development of molecular targeted treatments for LCs.

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Knockout of the Placenta Specific 8 Gene Affects the Proliferation and Migration of Human Embryonic Kidney 293T Cell

Cited by 7 publications

References 36 publications

Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers

Candidate oncogene placenta specific 8 affect cell growth and cell migration in non- small cell lung cancers

Multifaced roles of PLAC8 in cancer

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells

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