Knockout of the LW opsin gene interferes with oviposition selection of nocturnal moth, Helicoverpa armigera

Liu, X.; Cai, Limei; Zhu, Lin; Tian, Zhiqiang; Shen, Zhizhang; Cheng, Jie; Zhang, Songdou; Li, Zhen; Liu, Xiaoxia

doi:10.1007/s10340-022-01513-4

Cited by 3 publications

(6 citation statements)

References 50 publications

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“…Injection of dsRNA ( ZcRh3 or ZcRh4 ) reduced the phototactic efficiency of Z. cucurbitae to UV light. The results of the present study are similar to S. exigua , H. armigera , P. xylostella and D. citri 30–33 . In addition, dsEV_LWop and dsEV_Bop showed a higher inhibitory effect on the phototactic behavior in Empoasca vitis 79 .…”

Section: Discussionsupporting

confidence: 85%

“…The results of the present study are similar to S. exigua, H. armigera, P. xylostella and D. citri. [30][31][32][33] In addition, dsEV_LWop and dsEV_Bop showed a higher inhibitory effect on the phototactic behavior in Empoasca vitis. 79 LW-opsin and UV-opsin play a vital role in regulating the phototaxis in Z. cucurbitae due to their involvement and taxis to green and UV light.…”

Section: Discussionmentioning

confidence: 94%

“…Inhibition and knockdown of LW-opsin could reduce the sensitivity of S. exigua, Helicoverpa armigera and Plutella xylostella to green light. [30][31][32] Moreover, RNA interference of three opsin genes reduced the phototactic efficiency of Diaphorina citri to UV, green, and blue lights. 33 Z. cucurbitae is a significant agroeconomic pest that is present worldwide.…”

Section: Introductionmentioning

confidence: 99%

“…The research on the function of the opsin gene has become a hot topic. Inhibition and knockdown of LW‐opsin could reduce the sensitivity of S. exigua , Helicoverpa armigera and Plutella xylostella to green light 30–32 . Moreover, RNA interference of three opsin genes reduced the phototactic efficiency of Diaphorina citri to UV, green, and blue lights 33 …”

Section: Introductionmentioning

confidence: 99%

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Expression and function of opsin genes associated with phototaxis in Zeugodacus cucurbitae Coquillett (Diptera: Tephritidae)

Huang

Meng

Zhou

et al. 2023

Pest Management Science

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BackgroundZeugodacus cucuribitae is a major agricultural pest that causes significant damage to varieties of plants. Vision plays a critical role in phototactic behavior of herbivorous insects. However, the effect of opsin on the phototactic behavior in Z. cucuribitae remains unknown. The aim of this research is to explore the key opsin genes that associate with phototaxis behavior of Z. cucurbitae.ResultsFive opsin genes were identified and their expression patterns were analyzed. The relative expression levels of ZcRh1, ZcRh4 and ZcRh6 were highest in 4‐day‐old larvae, ZcRh2 and ZcRh3 were highest in 3rd‐instar larvae and 5‐day‐old pupae, respectively. Furthermore, five opsin genes had the highest expression levels in compound eyes, followed by the antennae and head, whereas the lower occurred in other tissues. The expression of the long‐wavelength‐sensitive (LW) opsins first decreased and then increased under green light exposure. In contrast, the expression of ultraviolet‐sensitive (UV) opsins first increased and then decreased with the duration of UV exposure. Silencing of LW opsin (dsZcRh1, dsZcRh2, and dsZcRh6) and UV opsin (dsZcRh3 and dsZcRh4) reduced the phototactic efficiency of Z. cucurbitae to green light by 52.27%, 60.72%, and 67.89%, and to UV light by 68.59% and 61.73%, respectively.ConclusionThe results indicate that RNAi inhibited the expression of opsin, thereby inhibiting the phototaxis of Z. cucurbitae. This result provides theoretical support for the physical control of Z. cucurbitae and lays the foundation for further exploration of the mechanism of insect phototaxis. © 2023 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and function of opsin genes associated with phototaxis in Zeugodacus cucurbitae Coquillett (Diptera: Tephritidae)

Huang

Meng

Zhou

et al. 2023

Pest Management Science

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“…Chen et al [ 4 ] knocked out LW2 opsin and observed the loss of LW2 opsin sensitivity to a long wavelength. Liu et al [ 14 ] knocked out the HaBL and HaLW genes using the CRISPR/Cas9 system, leading to a significant decrease in the phototaxis and the climbing of Helicoverpa armigera . Various opsin mutant strains developed based on genome editing technology show altered visual behavior, such as phototaxis [ 4 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Phototactic Changes in Phthorimaea absoluta Long-Wavelength Opsin Gene Mutants (LW2−/−) and Short-Wavelength Opsin Gene Mutant (BL−/−) Strains

Tang,

Wang,

Guo

et al. 2024

Insects

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Phthorimaea absoluta (Meyrick) is an invasive pest that has caused damage to tomatoes and other crops in China since 2017. Pest control is mainly based on chemical methods that pose significant threats to food safety and environmental and ecological security. Light-induced control, a green prevention and control technology, has gained attention recently. However, current light-trapping technology is non-specific, attracting targeted pests alongside natural enemies and non-target organisms. In this study, we characterized the phototactic behavior of tomato leaf miners for the development a specific light-trapping technology for pest control. In situ hybridization revealed opsin expression throughout the body. Furthermore, we investigated the tropism of pests (wild T. absoluta, Toxoptera graminum, and Bemisia tabaci) and natural enemies (Nesidiocoris tenuis and Trichogramma pintoi) using a wavelength-lamp tropism experiment. We found that 365 ± 5 nm light could accurately trap wild P. absoluta without trapping natural enemies and other insects. Finally, we analyzed the phototactic behavior of the mutant strains LW2(−/−) and BL(−/−). LW2 and BL mutants showed significant differences in phototactic behavior. The LW2(−/−) strain was attracted to light at 390 ± 5 nm and the BL(−/−) strain was unresponsive to any light. Our findings will help to develop specific light-trapping technology for controlling tomato leaf miners, providing a basis for understanding pest population dynamics and protecting crops against natural enemies.

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Genome editing: A novel approach to manage insect vectors of plant viruses

Jangra,

Potts,

Ghosh

et al. 2024

Insect Biochemistry and Molecular Biology

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Knockout of the LW opsin gene interferes with oviposition selection of nocturnal moth, Helicoverpa armigera

Cited by 3 publications

References 50 publications

Expression and function of opsin genes associated with phototaxis in Zeugodacus cucurbitae Coquillett (Diptera: Tephritidae)

Expression and function of opsin genes associated with phototaxis in Zeugodacus cucurbitae Coquillett (Diptera: Tephritidae)

Phototactic Changes in Phthorimaea absoluta Long-Wavelength Opsin Gene Mutants (LW2−/−) and Short-Wavelength Opsin Gene Mutant (BL−/−) Strains

Genome editing: A novel approach to manage insect vectors of plant viruses

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