Knockout mice reveal a role for protein tyrosine phosphatase H1 in cognition

Abstract

BackgroundThe present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions.MethodsWe have previously described a PTPH1-KO mouse, generated by replacing the PTP catalytic and the PDZ domain with a LacZ neomycin cassette. PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.ResultsIn CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons. The behavioral tests performed on the PTPH1-KO mice showed an impact on working memory in male mice and an impaired learning performance at rotarod in females.ConclusionThese results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.

The FERM and PDZ Domain-Containing Protein Tyrosine Phosphatases, PTPN4 and PTPN3, Are Both Dispensable for T Cell Receptor Signal Transduction

“…Consistent with a neural role, PTPN4-deficient mice were shown to have impaired cerebellar function, including defective motor learning [33] . In a recent report, PTPN3-deficient mice were also shown to have impaired memory function and motor learning, albeit that significant differences compared to controls were detected only in females [34] . Furthermore, in Drosophila , loss of expression of PTPMEG (the single homologue of PTPN3 and PTPN4 in this species) results in defective axon growth associated with impaired development of mushroom bodies [35] .…”