Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

Wolter, Felix; Puchta, Holger

doi:10.1186/s13059-017-1179-1

Cited by 48 publications

(29 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This enables the simultaneous induction of several DSBs without the need for multiple sgRNA expression cassettes. Mutation induction could be achieved in plants by applying Cas9 and its sgRNA coded as DNA, as RNA or directly via an RNP complex (for reviews see Altpeter et al [19] and Wolter and Puchta [20]). The targeting range of Cas9 is not limited to protein coding genes, but noncoding RNAs and regulatory elements can also be targeted [17,18].…”

Section: Using Cas9 In Plantsmentioning

confidence: 99%

“…Gene targeting (GT), the induction of precise genome alterations by HR, is still highly challenging in plants as NHEJ is the preferred mechanism for DSB repair in somatic plant cells and most crops are still lacking efficient transformation and regeneration procedures. Mutation induction could be achieved in plants by applying Cas9 and its sgRNA coded as DNA, as RNA or directly via an RNP complex (for reviews see Altpeter et al [19] and Wolter and Puchta [20]). A GT method independent on high transformation efficiencies is in planta GT.…”

Section: Using Cas9 As a Nucleasementioning

confidence: 99%

See 1 more Smart Citation

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

2018

Self Cite

View full text Add to dashboard Cite

Currently, biology is revolutionized by ever growing applications of the CRISPR/Cas system. As discussed in this Review, new avenues have opened up for plant research and breeding by the use of the sequence-specific DNases Cas9 and Cas12 (formerly named Cpf1) and, more recently, the RNase Cas13 (formerly named C2c2). Although double strand break-induced gene editing based on error-prone nonhomologous end joining has been applied to obtain new traits, such as powdery mildew resistance in wheat or improved pathogen resistance and increased yield in tomato, improved technologies based on CRISPR/Cas for programmed change in plant genomes via homologous recombination have recently been developed. Cas9- and Cas12- mediated DNA binding is used to develop tools for many useful applications, such as transcriptional regulation or fluorescence-based imaging of specific chromosomal loci in plant genomes. Cas13 has recently been applied to degrade mRNAs and combat viral RNA replication. By the possibility to address multiple sequences with different guide RNAs and by the simultaneous use of different Cas proteins in a single cell, we should soon be able to achieve complex changes of plant metabolism in a controlled way.

show abstract

Section: Using Cas9 In Plantsmentioning

confidence: 99%

Section: Using Cas9 As a Nucleasementioning

confidence: 99%

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, CRISPR/Cas9 may result in modified crops acceptable to those opposed to traditional GM techniques. For example, in 2015, Sweden confirmed that some plants edited using CRISPR/Cas9 were not considered genetically modified organisms (GMOs) under the European definition (Wolter and Puchta, 2017). One method for achieving further acceptance of crops modified using CRISPR/Cas9 is to avoid the use of foreign DNA, as recently achieved in maize (Svitashev et al, 2016) and bread wheat ( Triticum aestivum ) (Liang et al, 2017).…”

Section: Genomic Resources and Limitations: Mapping And Breedingmentioning

confidence: 99%

Exploiting Wild Relatives for Genomics-assisted Breeding of Perennial Crops

2017

View full text Add to dashboard Cite

Perennial crops are vital contributors to global food production and nutrition. However, the breeding of new perennial crops is an expensive and time-consuming process due to the large size and lengthy juvenile phase of many species. Genomics provides a valuable tool for improving the efficiency of breeding by allowing progeny possessing a trait of interest to be selected at the seed or seedling stage through marker-assisted selection (MAS). The benefits of MAS to a breeder are greatest when the targeted species takes a long time to reach maturity and is expensive to grow and maintain. Thus, MAS holds particular promise in perennials since they are often costly and time-consuming to grow to maturity and evaluate. Well-characterized germplasm that breeders can tap into for improving perennials is often limited in genetic diversity. Wild relatives are a largely untapped source of desirable traits including disease resistance, fruit quality, and rootstock characteristics. This review focuses on the use of genomics-assisted breeding in perennials, especially as it relates to the introgression of useful traits from wild relatives. The identification of genetic markers predictive of beneficial phenotypes derived from wild relatives is hampered by genomic tools designed for domesticated species that are often ill-suited for use in wild relatives. There is therefore an urgent need for better genomic resources from wild relatives. A further barrier to exploiting wild diversity through genomics is the phenotyping bottleneck: well-powered genetic mapping requires accurate and cost-effective characterization of large collections of diverse wild germplasm. While genomics will always be used in combination with traditional breeding methods, it is a powerful tool for accelerating the speed and reducing the costs of breeding while harvesting the potential of wild relatives for improving perennial crops.

show abstract

“…3c) [62]. More recently, several studies have highlighted significant improvements in genome editing in plants using DNA-free CRISPR/Cas9 ribonucleoproteins [65,98]. Besides its multiplexing qualities, CRISPR has also shown great efficiency to integrate large pathways and Simultaneous downregulation of 6 genes for fatty alcohol production [48] libraries [38,88].…”

Section: Genome Engineeringmentioning

confidence: 99%

Advancing biotechnology with CRISPR/Cas9: recent applications and patent landscape

Ferreira

David

Nielsen

2018

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Clustered regularly interspaced short palindromic repeats (CRISPR) is poised to become one of the key scientific discoveries of the twenty-first century. Originating from prokaryotic and archaeal immune systems to counter phage invasions, CRISPRbased applications have been tailored for manipulating a broad range of living organisms. From the different elucidated types of CRISPR mechanisms, the type II system adapted from Streptococcus pyogenes has been the most exploited as a tool for genome engineering and gene regulation. In this review, we describe the different applications of CRISPR/Cas9 technology in the industrial biotechnology field. Next, we detail the current status of the patent landscape, highlighting its exploitation through different companies, and conclude with future perspectives of this technology.

show abstract

Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

Abstract: Selection-free genome editing using Cas9 ribonucleoprotein embryo bombardment has been achieved for maize and wheat. This is a breakthrough that should make new breeding technologies more acceptable for worldwide use.

Cited by 48 publications

References 9 publications

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

Exploiting Wild Relatives for Genomics-assisted Breeding of Perennial Crops

Advancing biotechnology with CRISPR/Cas9: recent applications and patent landscape

Contact Info

Product

Resources

About