Knockdown of TMPRSS3 inhibits cell proliferation, migration/invasion and induces apoptosis of glioma cells

Huo, Jinxing; Chen, Xiaobing

doi:10.1002/jcb.28054

Cited by 5 publications

(5 citation statements)

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“…Furthermore, there is evidence linking the downregulation of 14‐3‐3 ζ protein, a distinct isoform of the YWHA protein family, with human GBM cells becoming more responsive to apoptosis induction 61 . For TM11B, the knockdown of another transmembrane protease serine family member, TMPRSS3, inhibited cell proliferation, migration, invasion, and induced apoptosis of glioma cells 62 …”

Section: Discussionmentioning

confidence: 99%

“… 61 For TM11B, the knockdown of another transmembrane protease serine family member, TMPRSS3, inhibited cell proliferation, migration, invasion, and induced apoptosis of glioma cells. 62 …”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, there is evidence linking the downregulation of 14-3-3 ζ protein, a distinct isoform of the YWHA protein family, with human GBM cells becoming more responsive to apoptosis induction 61. For TM11B, the knockdown of another transmembrane protease serine family member, TMPRSS3, inhibited cell proliferation, migration, invasion, and induced apoptosis of glioma cells 62. F I G U R E 4 (A) Box-and-whisker plots of normalized protein abundance of four protein biomarker candidates preoperatively (aldolase A [ALDOA], 14-3-3 protein ε, enoyl CoA hydratase 1, and transmembrane protease serine 11B).…”

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confidence: 99%

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Proteome profiling of salivary small extracellular vesicles in glioblastoma patients

Bark

Lima

Zhang

et al. 2023

Cancer

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BackgroundExtracellular vesicles (EVs) play a critical role in intercellular communication under physiological and pathological conditions, including cancer. EVs cargo reflects their cell of origin, suggesting their utility as biomarkers. EVs are detected in several biofluids, and their ability to cross the blood–brain barrier has highlighted their potential as prognostic and diagnostic biomarkers in gliomas, including glioblastoma (GBM). Studies have demonstrated the potential clinical utility of plasma‐derived EVs in glioma. However, little is known about the clinical utility of saliva‐derived EVs in GBM.MethodsSmall EVs were isolated from whole mouth saliva of GBM patients pre‐ and postoperatively. Isolation was performed using differential centrifugation and/or ultracentrifugation. EVs were characterized by concentration, size, morphology, and EVs cell‐surface protein markers. Protein cargo in EVs was profiled using mass spectrometry.ResultsThere were no statistically significant differences in size and concentration of EVs derived from pre‐ and post GBM patients' saliva samples. A higher number of proteins were detected in preoperative samples compared to postoperative samples. The authors found four highly abundant proteins (aldolase A, 14‐3‐3 protein ε, enoyl CoA hydratase 1, and transmembrane protease serine 11B) in preoperative saliva samples from GBM patients with poor outcomes. Functional enrichment analysis of pre‐ and postoperative saliva samples showed significant enrichment of several pathways, including those related to the immune system, cell cycle and programmed cell death.ConclusionsThis study, for the first time, demonstrates the feasibility of isolating and characterizing small EVs from pre‐ and postoperative saliva samples from GBM patients. Preliminary findings encourage further large cohort validation studies on salivary small EVs to evaluate prognosis in GBM.

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Section: Discussionmentioning

confidence: 99%

“… 61 For TM11B, the knockdown of another transmembrane protease serine family member, TMPRSS3, inhibited cell proliferation, migration, invasion, and induced apoptosis of glioma cells. 62 …”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Proteome profiling of salivary small extracellular vesicles in glioblastoma patients

Bark

Lima

Zhang

et al. 2023

Cancer

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“…The protein encoded by TMPRSS belongs to the serine protease family, a predominant factor in cancer progression and metastasis [ 32 ]. With regard to clinical significance, TMPRSS3 acts as a pro-oncogene in breast cancer [ 33 , 34 ], pancreatic cancer [ 35 ], glioma [ 36 ], gastric cancer [ 37 ], and nasopharyngeal cancer [ 38 ]. In the case of OV, initial studies revealed that TMPRSS3 expression was upregulated in OV tissues compared to normal tissues [ 39 ], while further research indicated that this gene promotes the proliferation, invasion, and metastatic ability of OV cells through the regulation of the ERK pathway [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Integration of Transcriptome and Epigenome to Identify and Develop Prognostic Markers for Ovarian Cancer

Cao

2022

Journal of Oncology

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DNA methylation is a widely researched epigenetic modification. It is associated with the occurrence and development of cancer and has helped evaluate patients’ prognoses. However, most existing DNA methylation prognosis models have not simultaneously considered the changes of the downstream transcriptome. Methods. The RNA-Sequencing data and DNA methylation omics data of ovarian cancer patients were downloaded from The Cancer Genome Atlas (TCGA) database. The Consensus Cluster Plus algorithm was used to construct the methylated molecular subtypes of the ovary. Lasso regression was employed to build a multi-gene signature. An independent data set was applied to verify the prognostic value of the signature. The Gene Set Variation Analysis (GSVA) was used to carry out the enrichment analysis of the pathways linked to the gene signature. The IMvigor 210 cohort was used to explore the predictive efficacy of the gene signature for immunotherapy response. Results. We distinguished ovarian cancer samples into two subtypes with different prognosis, based on the omics data of DNA methylation. Differentially expressed genes and enrichment analysis among subtypes indicated that DNA methylation was related to fatty acid metabolism and the extracellular matrix (ECM)-receptor. Furthermore, we constructed an 8-gene signature, which proved to be efficient and stable in predicting prognostics in ovarian cancer patients with different data sets and distinctive pathological characteristics. Finally, the 8-gene signature could predict patients’ responses to immunotherapy. The polymerase chain reaction experiment was further used to verify the expression of 8 genes. Conclusion. We analyzed the prognostic value of the related genes of methylation in ovarian cancer. The 8-gene signature predicted the prognosis and immunotherapy response of ovarian cancer patients well and is expected to be valuable in clinical application.

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BackgroundPrevious studies have shown that transmembrane protease serine 3 (TMPRSS3) is abnormally expressed in various tumours and its expression is closely related to tumorigenesis and progression [1][2][3]. However, there have been no reports regarding the role of TMPRSS3 in colorectal cancer (CRC).

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confidence: 99%

Upregulation of the Transmembrane Protease Serine 3 Mrna Level in Radioresistant Colorectal Cancer Tissues

et al. 2022

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Aim: To investigate the clinical role of transmembrane protease serine 3 (TMPRSS3) in radioresistance and prognosis of colorectal cancer (CRC). Methods: Standardized mean difference (SMD) and summary area under the curve (AUC) of TMPRSS3 were calculated by combining all available high-throughput data globally. The prognostic significance of TMPRSS3 was determined by Kaplan–Meier and Cox regression analyses. Results: TMPRSS3 was remarkably upregulated in 198 CRC radioresistant cases compared with nonradioresistance (SMD = 0.38, AUC = 0.71). Overexpression of TMPRSS3 was observed in 1601 CRC patients compared with control subjects without CRC. TMPRSS3 was a risk factor for disease-free survival of CRC with the summarized hazard ratio 1.28. Conclusion: TMPRSS3 contributes to the radioresistance and unfavorable prognosis of CRC.

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Knockdown of TMPRSS3 inhibits cell proliferation, migration/invasion and induces apoptosis of glioma cells

Cited by 5 publications

References 24 publications

Proteome profiling of salivary small extracellular vesicles in glioblastoma patients

Proteome profiling of salivary small extracellular vesicles in glioblastoma patients

Integration of Transcriptome and Epigenome to Identify and Develop Prognostic Markers for Ovarian Cancer

Upregulation of the Transmembrane Protease Serine 3 Mrna Level in Radioresistant Colorectal Cancer Tissues

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