Knockdown of the astrocytic glucocorticoid receptor in the central nucleus of the amygdala diminishes conditioned fear expression and anxiety

Wiktorowska, Lucja; Bilecki, Wiktor; Tertil, Magdalena; Kudla, Lucja; Szumiec, Łukasz; Maćkowiak, Marzena; Przewłocki, Ryszard

doi:10.1016/j.bbr.2020.113095

Cited by 12 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After each trial, both the chambers were cleaned by 70% alcohol to remove the odor of the previous animal. Decreased time spent, number of entries, and latency to re‐enter the light chamber were considered the indices of anxiety‐like behavior (Wiktorowska et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

Repeated finasteride administration promotes synaptic plasticity and produces antidepressant‐ and anxiolytic‐like effects in female rats

Nayana,

Shankaranarayana Rao,

Srikumar

2024

J of Neuroscience Research

View full text Add to dashboard Cite

Finasteride is used in female‐pattern hair loss, hirsutism, and polycystic ovarian syndrome. It inhibits 5α‐reductase, which is an important enzyme in the biosynthesis of neurosteroids. The effects of finasteride treatment on mental health in female patients as well as the effects of repeated/chronic finasteride administration in female rodents are still unknown. Accordingly, in our study, we administered finasteride (10, 30, or 100 mg/Kg, s.c.) for 6 days in female rats and evaluated behavior, plasma steroid levels, and synaptic plasticity. Depression‐like behavior was evaluated using forced swim test (FST) and splash test. Anxiety‐like behavior was evaluated using novelty‐suppressed feeding task (NSFT), elevated plus maze (EPM), open field test (OFT), and light–dark test (LDT). Plasma steroid levels were assessed using ELISA and synaptic plasticity by field potential recordings. We observed that finasteride decreased total immobility duration in FST, indicating antidepressant‐like effect and decreased the latency to first bite in NSFT, showing anxiolytic‐like effect. We also found a significant increase in plasma estradiol and a significant decrease in plasma corticosterone level. Furthermore, field potential recordings showed that finasteride increased hippocampal long‐term potentiation. These results indicate that repeated finasteride administration in female rats may have antidepressant‐ and anxiolytic‐like effect, which might be mediated by enhanced estradiol levels or decreased corticosterone levels. Further studies are required to validate the molecular mechanisms underlying the effects of finasteride in female rats. Understanding the mechanisms will help us in developing novel neurosteroid‐based therapeutics in the treatment of neuropsychiatric disorders in women.

show abstract

Section: Methodsmentioning

confidence: 99%

Repeated finasteride administration promotes synaptic plasticity and produces antidepressant‐ and anxiolytic‐like effects in female rats

Nayana,

Shankaranarayana Rao,

Srikumar

2024

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…Specific stimulation of astrocytes from mice’s medial basal hypothalamus suppresses food intake (Sweeney et al, 2016 ). Finally, mice lacking astrocyte glucocorticoid receptors in the amygdala show attenuated anxiety behaviors in the open field behavioral test and fear memory (Wiktorowska et al, 2021 ), demonstrating a direct involvement of astrocytes in fear memory and anxiety.…”

Section: Role Of Astrocytes In Glutamatergic Neurotransmission During...mentioning

confidence: 99%

Revealing the contribution of astrocytes to glutamatergic neuronal transmission

Cuellar-Santoyo

Ruiz-Rodríguez

Mares-Barbosa

et al. 2023

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Research on glutamatergic neurotransmission has focused mainly on the function of presynaptic and postsynaptic neurons, leaving astrocytes with a secondary role only to ensure successful neurotransmission. However, recent evidence indicates that astrocytes contribute actively and even regulate neuronal transmission at different levels. This review establishes a framework by comparing glutamatergic components between neurons and astrocytes to examine how astrocytes modulate or otherwise influence neuronal transmission. We have included the most recent findings about the role of astrocytes in neurotransmission, allowing us to understand the complex network of neuron-astrocyte interactions. However, despite the knowledge of synaptic modulation by astrocytes, their contribution to specific physiological and pathological conditions remains to be elucidated. A full understanding of the astrocyte’s role in neuronal processing could open fruitful new frontiers in the development of therapeutic applications.

show abstract

“…Neurons within the amygdala express a high density of corticosteroid receptors. The central nucleus of the amygdala can also facilitate the activation of the hypothalamic-pituitary-adrenal (HPA) axis in response to stress (Wiktorowska et al, 2021). Formaldehyde could increase glucocorticoid levels by triggering and exciting the HPA axis.…”

Section: Figure 3 Yellow Discoloration Of the Fur Caused By Formaldehydementioning

confidence: 99%

Effects of Occupational Formaldehyde Exposure on Passive Avoidance Conditioning and Anxiety Levels in Wistar rats

Resae

Yousefi²,

Naeimi³

et al. 2023

Iran J Vet Med

View full text Add to dashboard Cite

Background: Formaldehyde is a volatile organic compound widely used in industry and medical fields such as Anatomy and Pathology. Exposure to this chemical negatively affects the skin, mucous membrane, and respiratory system. It can pass through the blood-brain barrier, potentially causing neurotoxicity. According to studies, formaldehyde might be involved in memory impairment and the cognitive decline process in Alzheimer disease (AD). Objectives: This study aimed to simulate chronic occupational formaldehyde exposure in rats and study its impacts on passive avoidance conditioning and anxiety. Methods: Twenty-four adult male Wistar rats were divided into four groups of 6 rats each. After an adaptation period, the rats were exposed to 1, 2, and 3 ppm formaldehyde vapor in an exposure chamber, 6 hours per day for 7 days. The control group was exposed to saline. After the exposure period, a shuttle box for passive avoidance conditioning and an elevated plus-maze test for assessing anxiety levels were performed. The data were analyzed by 1-way ANOVA and Duncan’s multiple range test for group comparison in SPSS and SAS software. Results: In the shuttle box test, formaldehyde dose-dependently decreased escape-through latency and increased the percentage of dark compartment entries (P<0.0001). In the elevated plus maze test, the percentage of time spent in open arms decreased by increasing the dosage (P<0.0001). Conclusion: Based on these findings, formaldehyde exposure can negatively alter brain function and cause memory impairment and anxiety.

show abstract

Knockdown of the astrocytic glucocorticoid receptor in the central nucleus of the amygdala diminishes conditioned fear expression and anxiety

Cited by 12 publications

References 36 publications

Repeated finasteride administration promotes synaptic plasticity and produces antidepressant‐ and anxiolytic‐like effects in female rats

Repeated finasteride administration promotes synaptic plasticity and produces antidepressant‐ and anxiolytic‐like effects in female rats

Revealing the contribution of astrocytes to glutamatergic neuronal transmission

Effects of Occupational Formaldehyde Exposure on Passive Avoidance Conditioning and Anxiety Levels in Wistar rats

Contact Info

Product

Resources

About