Knockdown of sphingomyelinase (<scp><i>NlSMase</i></scp>) causes ovarian malformation of brown planthopper,<i>Nilaparvata lugens</i>(Stål)

Shi, Xiaoxiao; Zhang, He; Quais, Khairul; Chen, Ming; Wang, Ni; Zhang, Chao; Mao, Cungui; Zhu, Zhiliang

doi:10.1111/imb.12767

Cited by 3 publications

(1 citation statement)

References 46 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Vg has also been reported to be synthesized in follicular cells, nurse cells, and haemocytes [ 5–7 ]. Cumulative studies have demonstrated that female reproduction is regulated by numerous biological and abiotic factors, such as hormones, environmental temperature, nutrition, and metabolism [ 8–10 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis

et al. 2023

View full text Add to dashboard Cite

In female animals, metabolic homoeostasis and reproductive fitness are critical to population expansion. The trade-off between lipid storage and reproduction inevitably occurs. However, most studies have focused on the complex network of relationships between reproductive and metabolic physiology at the transcriptional level. In this study, we identified a microRNA, miR-2b-2-5p, in a highly invasive quarantine pest, Bactrocera dorsalis . Knockdown of miR-2b-2-5p by antagomiR microinjection impaired ovarian development, reduced fecundity, and decreased triglyceride (TAG) storage in the fat body, whereas overexpression of miR-2b-2-5p by injection of its mimic caused reproductive defects similar to knockdown but increased TAG. Bioinformatics analysis and dual luciferase assay indicated that cyclic AMP response element (CRE)-binding protein (CREB) was the target gene of miR-2b-2-5p. RNAi-mediated knockdown of CREB led to excessive lipid storage and reproductive defects. Further starvation treatment revealed that miR-2b-2-5p functions by fine-tuning CREB expression in response to dietary stimuli. These results suggest that miR-2b-2-5p acts as a monitor to regulate CREB mRNA levels in the fat body, maintaining lipid homoeostasis and keeping the reproductive system on track. Thus, our study not only provides new insights into the interaction between metabolism and reproduction at the posttranscriptional level in B. dorsalis , but also providing a potential eco-friendly control strategy (RNAi-based biopesticides targeting essential miRNAs) for this notorious agricultural pest.

show abstract

Section: Introductionmentioning

confidence: 99%