Knockdown of Notch Suppresses Epithelial-mesenchymal Transition and Induces Angiogenesis in Oral Submucous Fibrosis by Regulating TGF-β1

Wang, JinRong; Yang, Liyan; Mei, Jie; Li, Zhixin; Huang, Yu; Sun, Hong; Zheng, Kaiyue; Kuang, Huifang; Luo, Wen

doi:10.1007/s10528-023-10452-3

Cited by 2 publications

(1 citation statement)

References 40 publications

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“… 7 The expression of Notch was significantly upregulated in OSF tissues and TGF-β1-induced buccal mucosal fibroblasts. 8 NOTCH1 was identified as an independent prognostic factor for overall survival of early oral squamous cell carcinoma (OSCC). 9 Aberrant activation of Notch signaling was significantly associated with lymph node metastasis (LNM), invasion depth, and local recurrence in OSCC.…”

Section: Introductionmentioning

confidence: 99%

Jiawei Danxuan Koukang Alleviates Arecoline Induced Oral Mucosal Lesions: Network Pharmacology and the Combined Ultra-High Performance Liquid Chromatography (UPLC) and Mass Spectrometry (MS)

Zhou,

Tan,

Dai

et al. 2023

DDDT

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Purpose Arecoline is one of the main toxic components of arecoline to cause oral mucosal lesions or canceration, which seriously affects the survival and life quality of patients. This study analyzed the mechanism of Jiawei Danxuan Koukang (JDK) in alleviating arecoline induced oral mucosal lesions, to provide new insights for the treatment of oral submucosal fibrosis (OSF) or cancerosis. Methods Metabolomics was applied to analyze the composition of JDK and serum metabolites. The active ingredients of JDK were analyzed by the combined ultra-high performance liquid chromatography and mass spectrometry. The target network of JDK, metabolites and OSF was analyzed by network pharmacology, and molecular docking. Oral mucosal lesions and fibrosis were analyzed by HE and Masson staining. Cell differentiation, proliferation and apoptosis were detected. The expressions of α-SMA, Collagen I, Vimentin, Snail, E-cadherin, AR and NOTCH1 were detected by Western blot. Results Arecoline induced the gradual atrophy and thinning of rat oral mucosal, collagen accumulation, the increase expressions of fibrosis-related proteins and Th17/Treg ratio. JDK inhibited arecoline-induced oral mucosal lesions and inflammatory infiltration. Arecoline induced changes of serum metabolites in Aminoacyl-tRNA biosynthesis, Alanine, aspartate and glutamate metabolism and Arginine biosynthesis pathways, which were reversed by M-JDK. Quercetin and AR were the active ingredients and key targets of JDK, metabolites and OSF interaction. Arecoline promoted the expression of AR protein, and the proliferation of oral fibroblasts. Quercetin inhibited the effect of arecoline on oral fibroblasts, but was reversed by AR overexpression. Arecoline induced NOTCH1 expression in CAL27 and SCC-25 cells, and promoted cell proliferation, but was reversed by M-JDK or quercetin. Conclusion JDK improved the arecoline-induced OSF and serum metabolite functional pathway. Quercetin targeted AR protein to improve arecoline-induced OSF. JDK and quercetin inhibited arecoline-induced NOTCH1 protein expression in CAL27 and SCC-25 cells to play an anti-oral cancer role.

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Section: Introductionmentioning

confidence: 99%

Jiawei Danxuan Koukang Alleviates Arecoline Induced Oral Mucosal Lesions: Network Pharmacology and the Combined Ultra-High Performance Liquid Chromatography (UPLC) and Mass Spectrometry (MS)

Zhou,

Tan,

Dai

et al. 2023

DDDT

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Correlation relationships between the levels of microRNA and mRNA involved in pathological angiogenesis in experimental liver cirrhosis

Lebedeva,

Shchastniy,

Babenka

et al. 2024

jour

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Objective. To identify the relationships between the levels of microRNA and mRNA during pathological angiogenesis under the conditions of experimental toxic liver cirrhosis.Materials and methods. Fibrosis and liver cirrhosis were induced in male Wistar rats using a freshly prepared solution of thioacetamide over 17 weeks. The dynamics of the process were studied at 9 time points. The areas of interlobular veins and interlobular arteries were measured in micrometers. The numbers of interlobular arteries, interlobular veins, and sinusoidal capillaries were counted. The expression levels of mRNA genes Ang, Vegfa, Tweak, Fn14, Notch1, Notch2, and microRNAs-195-5p, microRNAs-664-3p, microRNAs-489-3p, microRNAs-3085, microRNAs-3558-3p in the liver were determined by real-time polymerase chain reaction.Results. It was found that during progressive venous angiogenesis, as well as against the background of changes in the number of interlobular veins, sinusoidal capillaries, and the area of interlobular veins, the nature and strength of correlational interactions between the genes Ang, Vegfa, Tweak, Fn14, Notch1, Notch2, and microRNAs-195-5p, microRNAs-664-3p, microRNAs-489-3p, microRNAs-3085, microRNAs-3558-3p are associated with the stage of fibrosis and liver cirrhosis. Strong, moderate, and mild correlational links (p<0.01) were identified between the genes Ang, Vegfa, Tweak, Fn14, Notch1, Notch2 throughout the experiment.Conclusion. The obtained results indicate that the studied microRNAs - microRNAs-195-5p, microRNAs-664-3p, microRNAs-489-3p, microRNAs-3085, microRNAs-3558-3p - are involved in the processes of restructuring the intrahepatic vascular bed at different stages of experimental toxic fibrosis and liver cirrhosis. The results of the study not only reveal additional mechanisms of formation of the indicated conditions but also identify the biological role of the studied microRNAs in the progression of fibrosis and liver cirrhosis as potential targets for studying pathogenesis and developing treatment methods.

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Knockdown of Notch Suppresses Epithelial-mesenchymal Transition and Induces Angiogenesis in Oral Submucous Fibrosis by Regulating TGF-β1

Cited by 2 publications

References 40 publications

Jiawei Danxuan Koukang Alleviates Arecoline Induced Oral Mucosal Lesions: Network Pharmacology and the Combined Ultra-High Performance Liquid Chromatography (UPLC) and Mass Spectrometry (MS)

Jiawei Danxuan Koukang Alleviates Arecoline Induced Oral Mucosal Lesions: Network Pharmacology and the Combined Ultra-High Performance Liquid Chromatography (UPLC) and Mass Spectrometry (MS)

Correlation relationships between the levels of microRNA and mRNA involved in pathological angiogenesis in experimental liver cirrhosis

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