Knockdown of microsomal glutathione S-transferase 1 inhibits lung adenocarcinoma cell proliferation and induces apoptosis

Zeng, Baozhen; Ge, Chao; Li, Ruilei; Zhang, Zhiwei; Fu, Qiangqiang; Li, Zhen; Lin, Zhuying; Liu, Lin; Xue, Yingwei; Xu, Yuanyuan; He, Jun; Guo, Huan; Li, Chunyan; Huang, Wenhua; Song, Xin; Huang, Yang H.

doi:10.1016/j.biopha.2019.109562

Cited by 32 publications

(30 citation statements)

References 41 publications

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“…The enzyme exhibits glutathione transferase and peroxidase activity, and shows activity against a variety of active substrates, from lipid peroxidation to cytostatic drugs ( 38 ). MGST1 is overexpressed in various cancers ( 38 , 39 ) and associated with drug resistance ( 37 ). Linnerth et al suggested that overexpression of MGST1 has been identified as an early marker in lung cancer ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

“…Linnerth et al suggested that overexpression of MGST1 has been identified as an early marker in lung cancer ( 40 ). Further, Zeng and his colleagues demonstrated MGST1 knockdown could inhibit lung adenocarcinoma cell proliferation by inactivating the AKT/GSK-3β pathway signaling and promote cell apoptosis by regulating the mitochondrial apoptosis pathway related proteins ( 39 ). Moreover, MGST1 overexpression was correlated to higher metastatic potential in human prostate cancer ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

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Prognostic Implications of Metabolism Related Gene Signature in Cutaneous Melanoma

Zeng

Peng

et al. 2020

Front. Oncol.

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Metabolic reprogramming is closely related to melanoma. However, the prognostic role of metabolism-related genes (MRGs) remains to be elucidated. We aimed to establish a nomogram by combining MRGs signature and clinicopathological factors to predict melanoma prognosis. Eighteen prognostic MRGs between melanoma and normal samples were identified using The Cancer Genome Atlas (TCGA) and GSE15605. WARS (HR = 0.881, 95% CI = 0.788-0.984, P = 0.025) and MGST1 (HR = 1.124, 95% CI = 1.007-1.255, P = 0.037) were ultimately identified as independent prognostic MRGs with LASSO regression and multivariate Cox regression. The MRGs signature was established according to these two genes and externally validated in the Gene Expression Omnibus (GEO) dataset. Kaplan-Meier survival analysis indicated that patients in the high-risk group had significantly poorer overall survival (OS) than those in the low-risk group. Furthermore, the MRGs signature was identified as an independent prognostic factor for melanoma survival. An MRGs nomogram based on the MRGs signature and clinicopathological factors was developed in TCGA cohort and validated in the GEO dataset. Calibration plots showed good consistency between the prediction of nomogram and actual observation. The receiver operating characteristic curve and decision curve analysis indicated that MRGs nomogram had better OS prediction and clinical net benefit than the stage system. To our knowledge, we are the first to develop a prognostic nomogram based on MRGs signature with better predictive power than the current staging system, which could assist individualized prognosis prediction and improve treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prognostic Implications of Metabolism Related Gene Signature in Cutaneous Melanoma

Zeng

Peng

et al. 2020

Front. Oncol.

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“…78,79 Studies have shown that MGST1 is upregulated in various cancer types, such as lung, prostate, brain, and colorectal, 79 and linked its expression to tumorigenesis and apoptosis. 80 Recently, a study of genetic variations in inflammation-related pathways found that MGST1 variants were associated with increased risk of BE and EA in European populations. 57 LMO members are important regulators in cell fate determination and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Sex-Specific Genetic Associations for Barrett’s Esophagus and Esophageal Adenocarcinoma

et al. 2020

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BACKGROUND & AIMS: Esophageal adenocarcinoma (EA) and its premalignant lesion, Barrett's esophagus (BE), are characterized by a strong and yet unexplained male predominance (with a male-to-female ratio in EA incidence of up to 6:1). Genome-wide association studies (GWAS) have identified more than 20 susceptibility loci for these conditions. However, potential sex differences in genetic associations with BE/EA remain largely unexplored. METHODS: Given strong genetic overlap, BE and EA cases were combined into a single case group for analysis. These were compared with populationbased controls. We performed sex-specific GWAS of BE/EA in 3 separate studies and then used fixed-effects meta-analysis to provide summary estimates for >9 million variants for male and female individuals. A series of downstream analyses were conducted separately in male and female individuals to identify genes associated with BE/EA and the genetic correlations

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“…A total of 30 µl of the secondary antibody in the DAB kit (Dako; Agilent Technologies, Inc.), with original concentration, was added to the sections and incubated at 37˚C for 30 min. H&E staining was used to evaluate histology (32). Paraffin-embedded tissues samples were cut into 4-µm-thick sections and incubated at 60˚C for 1 h. The samples were subsequently deparaffinized in xylene at 60˚C for 15 min and rehydrated prior to incubation with hematoxylin (original concentration; Fuzhou Maixin Biotech Co., Ltd.) at room temperature for 2 min, followed by incubation with eosin (original concentration; Guangzhou RiboBio Co., Ltd.) at room temperature for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Increased expression of the RNA‑binding motif protein 47�predicts poor prognosis in non‑small‑cell lung cancer

et al. 2020

Oncol Lett

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Lung cancer is the leading cause of cancer-associated mortality worldwide. In China, in particular, lung cancer mortality has markedly increased and is likely to continue to rise. RNA-binding proteins are pivotal to the development and progression of a variety of cancer types, including non-small cell lung cancer (NSCLC). RNA-binding motif protein 47 (RBM47) has been found to act as a tumor suppressor in breast cancer and NSCLC. However, to the best of our knowledge, RBM47 expression in NSCLC tissues has yet to be investigated. Analysis via the online database, Gene Expression Omnibus, revealed that RBM47 was upregulated in NSCLC and associated with pathological type, suggesting that RBM47 may play different roles in lung adenocarcinoma and lung squamous cell carcinoma. In the present study, the expression of RBM47 was examined by immunohistochemistry in 175 pairs of tumor and adjacent non-cancerous tissues resected from patients with NSCLC. The results indicated that the expression of RBM47 was significantly increased in NSCLC samples compared with that in the matched non-cancerous samples. Furthermore, RBM47 expression was higher in Xuanwei compared with that in non-Xuanwei NSCLC, suggesting that RBM47 is a more sensitive biomarker in Xuanwei NSCLC, and that it may serve as a candidate therapeutic target. In addition, RBM47 expression was associated with the pathological type, however not with the age, sex, lymph node metastasis, pT stage or pathological Tumor-Node-Metastasis stage of the patients. The increased expression level of RBM47 may indicate a worse overall survival rate for patients with NSCLC. In addition, multivariate survival analysis showed that the Xuanwei area is associated with poor prognosis for patients with NSCLC. In conclusion, the present study revealed that the upregulation of RBM47 accelerated the malignant progression of NSCLC, indicating that RBM47 may be a potential biomarker for NSCLC progression and a therapeutic target for NSCLC.

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Knockdown of microsomal glutathione S-transferase 1 inhibits lung adenocarcinoma cell proliferation and induces apoptosis

Cited by 32 publications

References 41 publications

Prognostic Implications of Metabolism Related Gene Signature in Cutaneous Melanoma

Prognostic Implications of Metabolism Related Gene Signature in Cutaneous Melanoma

Sex-Specific Genetic Associations for Barrett’s Esophagus and Esophageal Adenocarcinoma

Increased expression of the RNA‑binding motif protein 47�predicts poor prognosis in non‑small‑cell lung cancer

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