Knockdown of lncRNA MIR503HG suppresses proliferation and promotes apoptosis of non-small cell lung cancer cells by regulating miR-489-3p and miR-625-5p

Dao, Runa; Wudu, Muli; Hui, Lian; Jiang, Jun; Xu, Yitong; Ren, Hongjiu; Qiu, Xueshan

doi:10.1016/j.prp.2020.152823

Cited by 33 publications

(25 citation statements)

References 29 publications

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“…Unlike miR-541-3p [ 30 , 31 ], miR-541-5p has never been studied in cancers. MiR-625-5p and miR-149-5p have been previously reported to have tumor suppressive roles in various cancers, alone or combined with various chemotherapeutic agents [ 32 , 33 , 34 , 35 , 36 ]. Even if miR-149-5p seems to play a dual role in cancer [ 37 ], both miR-625-5p and miR-149-5p can induce apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Tumor Suppressive Role of miR-342-5p in Human Chondrosarcoma Cells and 3D Organoids

Veys

Benmoussa

Contentin

et al. 2021

IJMS

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Chondrosarcomas are malignant bone tumors. Their abundant cartilage-like extracellular matrix and their hypoxic microenvironment contribute to their resistance to chemotherapy and radiotherapy, and no effective therapy is currently available. MicroRNAs (miRNAs) may be an interesting alternative in the development of therapeutic options. Here, for the first time in chondrosarcoma cells, we carried out high-throughput functional screening using impedancemetry, and identified five miRNAs with potential antiproliferative or chemosensitive effects on SW1353 chondrosarcoma cells. The cytotoxic effects of miR-342-5p and miR-491-5p were confirmed on three chondrosarcoma cell lines, using functional validation under normoxia and hypoxia. Both miRNAs induced apoptosis and miR-342-5p also induced autophagy. Western blots and luciferase reporter assays identified for the first time Bcl-2 as a direct target of miR-342-5p, and also Bcl-xL as a direct target of both miR-342-5p and miR-491-5p in chondrosarcoma cells. MiR-491-5p also inhibited EGFR expression. Finally, only miR-342-5p induced cell death on a relevant 3D chondrosarcoma organoid model under hypoxia that mimics the in vivo microenvironment. Altogether, our results revealed the tumor suppressive activity of miR-342-5p, and to a lesser extent of miR-491-5p, on chondrosarcoma lines. Through this study, we also confirmed the potential of Bcl-2 family members as therapeutic targets in chondrosarcomas.

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Section: Discussionmentioning

confidence: 99%

Tumor Suppressive Role of miR-342-5p in Human Chondrosarcoma Cells and 3D Organoids

Veys

Benmoussa

Contentin

et al. 2021

IJMS

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“…A previous study revealed that lncRNA miR503HG was downregulated in OC, and downregulation of miR503HG predicted poor survival of OC patients (Zhu et al, 2020 ), which coincided with miR503HG in our prognostic risk model signature. Coincidentally, the expression of MIR503HG was decreased in colon cancer (Chuo et al, 2019 ; Han H. et al, 2020 ), triple-negative breast cancer (Fu et al, 2019 ; Tuluhong et al, 2020 ), non-small cell lung cancer (Lin et al, 2019 ; Dao et al, 2020 ; Xu et al, 2020 ), cervical squamous cell carcinoma (Zhao et al, 2020 ), bladder cancer (Qiu et al, 2019 ), and hepatocellular carcinoma (Wang et al, 2018 ). MIR503HG was also proved to serve as a tumor suppressor in in vivo experiment.…”

Section: Discussionmentioning

confidence: 99%

Four Prognosis-Associated lncRNAs Serve as Biomarkers in Ovarian Cancer

et al. 2021

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Long non-coding RNAs (lncRNAs) play crucial roles in ovarian cancer (OC) development. However, prognosis-associated lncRNAs (PALs) for OC have not been completely elucidated. Our study aimed to identify the PAL signature of OC. A total of 663 differentially expressed lncRNAs were identified in the databases. According to the weighted gene coexpression analysis, the highly correlated genes were clustered into seven modules related to the clinical phenotype of OC. A total of 25 lncRNAs that were significantly related to overall survival were screened based on univariate Cox regression analysis. The prognostic risk model constructed contained seven PALs based on the parameter λmin, which could stratify OC patients into two risk groups. The results showed that the risk groups had different overall survival rates in both The Cancer Genome Atlas (TCGA) and two verified Gene Expression Omnibus (GEO) databases. Univariate and multivariate Cox regression analyses confirmed that the risk model was an independent risk factor for OC. Gene enrichment analysis revealed that the identified genes were involved in some pathways of malignancy. The competitive endogenous RNA (ceRNA) network included five PALs, of which four were selected for cell function assays. The four PALs were downregulated in 33 collected OC tissues and 3 OC cell lines relative to the control. They were shown to regulate the proliferative, migratory, and invasive potential of OC cells via Cell Counting Kit-8 (CCK-8) and transwell assays. Our study fills the gaps of the four PALs in OC, which are worthy of further study.

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“…In vitro studies suggested that the influence of MIR503HG on migration and invasion might be through miR-103 and OLFM4; authors concluded that MIR503HG functioned as a tumor-suppressive long-non coding RNA in TNBC 15 . MIR503HG expression has also been found to be dysregulated in non-small cell lung cancer; MIR503HG expression was significantly up-regulated in NSCLC cell lines, and unlike other cancer types studied, over-expression of MIR503HG enhanced proliferation and impaired apoptosis in NCI-H1975 NSCLC cells 16 . Mechanistically, authors argued MIR503HG might PAGE OF function by negatively regulating expression of miR-489-3p and miR-625-5p 16 .…”

Section: Mir503hg Significantly Increased Invasion and Migration Of Hcc Cell Lines Smmc-7221 And Page Ofmentioning

confidence: 96%

MIR503HG is differentially expressed in high-grade serous ovarian cancers.

Mamoor¹

2020

Preprint

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Knockdown of lncRNA MIR503HG suppresses proliferation and promotes apoptosis of non-small cell lung cancer cells by regulating miR-489-3p and miR-625-5p

Cited by 33 publications

References 29 publications

Tumor Suppressive Role of miR-342-5p in Human Chondrosarcoma Cells and 3D Organoids

Tumor Suppressive Role of miR-342-5p in Human Chondrosarcoma Cells and 3D Organoids

Four Prognosis-Associated lncRNAs Serve as Biomarkers in Ovarian Cancer

MIR503HG is differentially expressed in high-grade serous ovarian cancers.

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