Knockdown of lncRNA CCEPR suppresses colorectal cancer progression

Abstract

Long non-coding RNAs (lncRNAs) serve important roles in colorectal cancer. The aim of the present study was to investigate the expression and role of cervical carcinoma expressed PCNA regulatory (CCEPR) lncRNA in colorectal cancer progression. The results demonstrated that CCEPR expression was significantly higher in colorectal cancer tissues when compared with paired adjacent normal tissues. In addition, CCEPR expression was significantly higher in patients with advanced colorectal cancer (stage III/IV) than those with early-stage colorectal cancer (stage I/II). High CCEPR expression was significantly associated with poor differentiation, advanced clinical stage, positive lymph node metastasis and distant metastasis. Of particular note, patients with colorectal cancer exhibiting high CCEPR expression levels had shorter survival rates when compared with patients with low CCEPR expression. In vitro experiments demonstrated that the expression of CCEPR was increased in colorectal cancer cell lines when compared with a normal colon cell line. Knockdown of CCEPR significantly inhibited colorectal cancer cell proliferation, colony formation and cell cycle progression, as well as cell migration and invasion. Finally, silencing of CCEPR downregulated matrix metalloproteinase (MMP)-2 and MMP-9 expression and suppressed epithelial-mesenchymal transition in colorectal cancer cells. In conclusion, the results of the present study suggest that CCEPR may exert an oncogenic role in colorectal cancer, and CCEPR may be a promising molecular target for colorectal cancer treatment.

The lncRNA TERC promotes gastric cancer cell proliferation, migration, and invasion by sponging miR-423-5p to regulate SOX12 expression

“…Further, the results of the current study demonstrated that the knockdown of the lncRNA TERC significantly increased the proliferation, migration, and invasion of the HGC-27 and SNU-1 GC cell lines. Numerous previous studies have reported that PCNA, EMT, and MMP9 play important roles in the proliferation, migration, and invasion of cancer cells (38)(39)(40)(41)(42)(43). Notably, in the present study, the results demonstrated that the lncRNA TERC knockdown significantly upregulated the expression levels of the EMT-related marker, E-cadherin, and downregulated the expression levels of PCNA, MMP9, and N-cadherin in the HGC-27 and SNU-1 GC cell lines.…”

Overexpression of long non-coding RNA00355 enhances proliferation, chemotaxis, and metastasis in colon cancer via promoting GTF2B-mediated ITGA2

“…More importantly, the present study found that blocking lncRNA LINC00355 expression arrested the proliferation, invasion and migration of colon cancer cells and promoted apoptosis. Likewise, a previous study has validated that inhibition of the lncRNA CCEPR impedes invasion, proliferation, migration and cell cycle progression in colon cancer cells [32] . Similarly, suppression of the lncRNA AWPPH expression could suppress cell proliferation in colon cancer [33] .…”

Insights into the Role of Matrix Metalloproteinases in Precancerous Conditions and in Colorectal Cancer

“…A recent investigation showed a meaningful correlation between the overexpression of LINC00963 and the upregulation of MMP-2 and MMP-9 , proliferation, migration, and invasion of CRC cells [ 147 ]. Duan et al, revealed that the inhibition of the CCEPR lncRNA reduced the expression levels of MMP-2 and MMP-9 , and prevented EMT in CRC cells [ 148 ]. Pan et al, realized that the expression level of MMP-2 protein was notably decreased when PCA3 was knocked out.…”