Knockdown of LINC00511 decreased cisplatin resistance in non-small cell lung cancer by elevating miR-625 level to suppress the expression of leucine rich repeat containing eight volume-regulated anion channel subunit E

Abstract

Background LINC00511 has been reported as a biomarker related to the prognosis of non-small cell lung cancer (NSCLC), but the molecular mechanism and exact functions of LINC00511 in chemoresistance of NSCLC remain to be elucidated. Methods RT-qPCR was used to evaluate the mRNA expression of LINC00511, miR-625, and leucine rich repeat containing 8 volume-regulated anion channel subunit E (LRRC8E). Western blotting detected the protein levels of Ki-67, MMP-9, cleaved-caspase-3. The interaction between miR-625 and LINC00511 or LRRC8E was verified by luciferase reporter assays. CCK-8, TUNEL, and Transwell assays were used to evaluate IC50 value, proliferation, migration, and invasion of NSCLC cells. Results In our study, it was discovered that the levels of LINC00511 and LRRC8E were increased, while miR-625 expression was decreased in NSCLC tissues, DDP-resistant NSCLC cells, and non-resistant NSCLC cells. LINC00511 depletion significantly curbed cell growth, IC50 value, and metastasis in DDP-resistant NSCLC cells. In addition, the influence of LINC00511 deficiency on the DDP resistance in NSCLC was overturned by suppressing miR-625. Furthermore, LRRC8E overexpression abolished the promotive effect of miR-625 abundance on the DDP sensitivity in DDP-resistant NSCLC cells. Conclusion Our results demonstrated that LINC00511 increased DDP resistance in NSCLC by suppressing miR-625 to upregulate LRRC8E.

Human Adult Renal Progenitor Cells Prevent Cisplatin-Nephrotoxicity by Inducing CYP1B1 Overexpression and miR-27b-3p Down-Regulation through Extracellular Vesicles

“…In turn, hypoxia upregulates CYP1B1 [ 45 ]. However, LINC00511 has been found to support cisplatin resistance in non-small-cell lung cancer by elevating miR-625 level [ 46 ].…”

A review on the role of LINC00511 in cancer

“…Besides, this lncRNA has a role in induction of tumor recurrence in this type of cancer through sponging miR-98-5p and increasing expression of TGFBR1 (Li et al, 2022). LINC00511 can also induce resistance of lung cancer cells to Frontiers in Genetics frontiersin.org cisplatin through sponging miR-625 and influencing expression of LRRC8E (Liu et al, 2022). Finally, this lncRNA exerts its oncogenic effects in lung cancer through binding to EZH2 and decaresing expression of p57 (Sun et al, 2016).…”

Long non-coding RNA and Evolving drug resistance in lung cancer

“…LINC00511 overexpression protects BIRC5 mRNA from miR-182-3p-mediated inhibition and leads to apoptosis resistance in A549/DDP cells [ 45 ]. However, LINC00511 knockdown attenuates resistance to cisplatin by interacting with miR-625 and leucine rich repeat containing 8 volume-regulated anion channel subunit E (LRRC8E) [ 46 ].…”