Knockdown of ecdysone receptor in male desert locusts affects relative weight of accessory glands and mating behavior

Lommel, Joachim Van; Lenaerts, Cynthia; Delgouffe, Charlotte; Broeck, Jozef Vanden

doi:10.1016/j.jinsphys.2022.104368

Cited by 4 publications

(1 citation statement)

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“…The results on temporal expression showed that this gene was highly expressed in the Malpighian tubule and epidermis. Characteristic tissue and temporal expression patterns of EcR isoforms also have been reported in Drosophlia and other arthropods, such as silkworms, tobacco hornworms and locusts (Jindra & Malone, Hiruma, et al, 1996; Kamimura et al, 1997; Van Lommel et al, 2022; Perera et al, 1999; Talbot et al, 1993). Furthermore, in C .…”

Section: Discussionmentioning

confidence: 77%

Molecular characterization and functional analysis of the ecdysone receptor isoform (EcR) from the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Cao,

Bakumenko,

Vlasenko

et al. 2024

Arch Insect Biochem Physiol

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Abstract20‐Hydroxyecdysone (20E) plays a vital role in a series of biological processes, via the nuclear receptors, EcR/USP by activating the ecdysone regulatory cascade. To clarify the role of EcR during the development of Grapholita molesta, the complementary DNA of ecdysone receptor isoform B1 (GmEcR‐B1) was obtained from the transcriptome of G. molesta and verified by PCR. Alignment analysis revealed that the deduced protein sequence of GmEcR‐B1 was highly homologous to EcR proteins identified in other lepidopteran species, especially the EcR‐B1 isoform in Spodoptera litura. Quantitative real‐time PCR showed that GmEcRs was expressed at all test developmental stages, and the expression level of GmEcRs was relatively higher during the period of the 3rd day of fifth instar larvae to 2nd of pupa than those in other stages. Moreover, the messenger RNA of GmEcRs was much more strongly expressed in the Malpighian tubule and epidermis than those in other tissues, which suggests that this gene may function in a tissue‐specific manner during larval development. Silencing of GmEcRs could significantly downregulate the transcriptional level of ecdysone‐inducible genes and result in increased mortality during metamorphosis and prolonged prepupal duration. Taken together, the present results indicate that GmEcRs may directly or indirectly affect the development of G. molesta.

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