2022
DOI: 10.1002/jcp.30806
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Knockdown of E1‐ and E2‐ubiquitin enzymes triggers defective chorion biogenesis and modulation of autophagy‐related genes in the follicle cells of the vector Rhodnius prolixus

Abstract: In insects, the last stage of oogenesis is the process where the chorion layers (eggshell) are synthesized and deposited on the surface of the oocytes by the follicle cells. Protein homeostasis is determined by the fine‐tuning of translation and degradation pathways, and the ubiquitin–proteasome system is one of the major degradative routes in eukaryotic cells. The conjugation of ubiquitin to targeted substrates is mediated by the ordered action of E1‐activating, E2‐conjugating, and E3‐ligase enzymes, which co… Show more

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Cited by 8 publications
(22 citation statements)
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“…Within the ovary, all mRNAs tended to be more expressed in the tropharium, suggesting that this site is indeed the main source of maternal mRNA synthesis in the ovary and that cytoplasmic bridges are used to deliver the mRNAs to the transcriptionally inactive developing oocytes (Kunkel, 1991). This pattern, of highest expression levels in the tropharium, has been observed before in R. prolixus for genes of the autophagy pathway (Vieira et al, 2018;Bomfim and Ramos, 2020;Pereira et al, 2020), the ubiquitin-proteasome system (Pereira et al, 2022) and the unfolded protein response (Rios et al, 2022). It is important to notice, however, that although the temperature, humidity, light/dark cycles, and blood feeding intervals for each insect in this study were strictly controlled, it is expected that adult insects develop certain phenotypes during their early life.…”
Section: Discussionsupporting
confidence: 61%
“…Within the ovary, all mRNAs tended to be more expressed in the tropharium, suggesting that this site is indeed the main source of maternal mRNA synthesis in the ovary and that cytoplasmic bridges are used to deliver the mRNAs to the transcriptionally inactive developing oocytes (Kunkel, 1991). This pattern, of highest expression levels in the tropharium, has been observed before in R. prolixus for genes of the autophagy pathway (Vieira et al, 2018;Bomfim and Ramos, 2020;Pereira et al, 2020), the ubiquitin-proteasome system (Pereira et al, 2022) and the unfolded protein response (Rios et al, 2022). It is important to notice, however, that although the temperature, humidity, light/dark cycles, and blood feeding intervals for each insect in this study were strictly controlled, it is expected that adult insects develop certain phenotypes during their early life.…”
Section: Discussionsupporting
confidence: 61%
“…Such regulations were observed before in R . prolixus , where the silencing of the E1-activation ubiquitin enzyme also resulted in the induction of p62/SQSTM1 in the ovaries [ 49 ], as well as in HEK293 cells, where it was demonstrated that proteasome inhibition triggers the induction of p62/SQSTM1 and ATG8/GABARAP [ 105 ] while the proteasome itself can be targeted for autophagic degradation [ 106 ]. Moreover, the fact that both autophagy and UPS genes showed general tendencies toward upregulation in silenced insects’ ovaries further supports our findings that, although the reduced proteasomal activity was sufficient to induce the expression of autophagy and UPS genes, the elicited response was insufficient to initiate the UPR.…”
Section: Discussionmentioning
confidence: 99%
“…In the kissing bug R . prolixus , RNAi knockdown of the ubiquitin enzymes E1 and E2 resulted in impaired oogenesis and embryo lethality [ 49 ].…”
Section: Introductionmentioning
confidence: 99%
“…Relative expressions were obtained using the mean of Ct duplicates of the endogenous Rp18s (RPRC017412) and experimental genes. The 18s gene was used as the single reference gene because there is previous evidence of its invariable expression under our experimental conditions ( Bomfim and Ramos, 2020 ; Vieira et al, 2021 ; Pereira et al, 2022 ). The invariable expression of 18s specifically after the silencing of Sec16 is shown in Supplementary Figure S1 .…”
Section: Methodsmentioning
confidence: 99%