Knockdown of CYP301B1 and CYP6AX1v2 increases the susceptibility of the brown planthopper to beta-asarone, a potential plant-derived insecticide

Xu, Xueliang; Li, Xiang; Liu, Zirong; Wang, Fenshan; Fan, Linjuan; Wu, Caiyun; Yao, Yingjuan

doi:10.1016/j.ijbiomac.2020.12.217

Cited by 16 publications

(7 citation statements)

References 50 publications

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“…However, some genes in the CYP2 and Mito clans have also been found to have the ability to metabolize xenobiotics, which can help insects survive. For example, CYP18A1 in the CYP2 clan could epoxidize aldrin to dieldrin and CYP301B1 in the Mito clan enhances the resistance of N. lugens to plant-derived insecticide substances [ 77 , 91 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Zhang

Wang

et al. 2023

Insects

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Cytochrome P450 monooxygenases (P450s) have a variety of functions, including involvement in the metabolism of exogenous substances and the synthesis and degradation of endogenous substances, which are important for the growth and development of insects. Pseudoregma bambucicola is a social aphid that produces genetically identical but morphologically and behaviorally distinct first-instar soldiers and normal nymphs within colonies. In this study, we identified 43 P450 genes based on P. bambucicola genome data. Phylogenetic analysis showed that these genes were classified into 4 clans, 13 families, and 23 subfamilies. The CYP3 and CYP4 clans had a somewhat decreased number of genes. In addition, differential gene expression analysis based on transcriptome data showed that several P450 genes, including CYP18A1, CYP4G332, and CYP4G333, showed higher expression levels in soldiers compared to normal nymphs and adult aphids. These genes may be candidates for causing epidermal hardening and developmental arrest in soldiers. This study provides valuable data and lays the foundation for the study of functions of P450 genes in the social aphid P. bambucicola.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Zhang

Wang

et al. 2023

Insects

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“…This is a direct indication that TcCYP6BQ8 may be involved in the metabolic detoxification of toxic substances from plants. Similarly, mortality was significantly higher in Nilaparvata lugens (Stål) treated with β-asarone after silencing CYP6AX1 compared with the control group (Xu et al ., 2021). Silencing of CYP4PR1 in Triatoma infestans (Klug) followed by treatment with two different doses of deltamethrin significantly increased mortality compared with the control treatment (Dulbecco et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 gene CYP6BQ8 mediates terpinen-4-ol susceptibility in the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

Gao

Guo

Shu-mei

et al. 2023

Bull. Entomol. Res.

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Cytochrome P450 proteins (CYPs) in insects can encode various detoxification enzymes and catabolize heterologous substances, conferring tolerance to insecticides. This study describes the identification of a P450 gene (CYP6BQ8) from Tribolium castaneum (Herbst) and investigation of its spatiotemporal expression profile and potential role in the detoxification of terpinen-4-ol, a component of plant essential oils. The developmental expression profile showed that TcCYP6BQ8 expression was relatively higher in early- and late-larval stages of T. castaneum compared with other developmental stages. Tissue expression profiles showed that TcCYP6BQ8 was mainly expressed in the head and integument of both larvae and adults. The expression profiling of TcCYP6BQ8 in developmental stages and tissues is closely related to the detoxification of heterologous substances. TcCYP6BQ8 expression was significantly induced after exposure to terpinen-4-ol, and RNA interference against TcCYP6BQ8 increased terpinen-4-ol-induced larval mortality from 47.78 to 66.67%. This indicates that TcCYP6BQ8 may be involved in T. castaneum's metabolism of terpinen-4-ol. Correlation investigation between the CYP6BQ8 gene and terpinen-4-ol resistance in T. castaneum revealed that the TcCYP6BQ8 gene was one of the factors behind T. castaneum's resistance to terpinen-4-ol. This discovery may provide a new theoretical foundation for future regulation of T. castaneum.

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“…Plant-derived insecticides, especially plant metabolites, have become the subject of much research because they are safe, efficient, and easily biodegradable. 29 For example, natural pyrethrins are a class of specialized metabolites produced in Tanacetum cinerariifolium. 30 Their biological activities, insect repellent properties, and biodegradability have contributed to the development of pyrethrins as plant-derived insecticides for the control of flying insects.…”

Section: ■ Discussionmentioning

confidence: 99%

Esterification with a Long-Chain Fatty Acid Elevates the Exposure Toxicity of Tigliane Diterpenoids from Euphorbia fischeriana Roots against Nematodes

Zhang,

Liu,

et al. 2023

J. Agric. Food Chem.

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Knockdown of CYP301B1 and CYP6AX1v2 increases the susceptibility of the brown planthopper to beta-asarone, a potential plant-derived insecticide

Cited by 16 publications

References 50 publications

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social Aphid Pseudoregma bambucicola

Cytochrome P450 gene CYP6BQ8 mediates terpinen-4-ol susceptibility in the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

Esterification with a Long-Chain Fatty Acid Elevates the Exposure Toxicity of Tigliane Diterpenoids from Euphorbia fischeriana Roots against Nematodes

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