Knockdown of circHomer1 ameliorates METH-induced neuronal injury through inhibiting Bbc3 expression

Li, Junwei; Sun, Qiuhong; Zhu, Shirley; Xi, Kaiyan; Shi, Qingqing; Pang, Kunkun; Liu, Xiaoyu; Li, Meng; Zhang, Yue; Sun, Jinhao

doi:10.1016/j.neulet.2020.135050

Cited by 16 publications

(14 citation statements)

References 39 publications

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“…12 Additionally, emerging evidence suggests that circ_0006916 knockdown mitigates methamphetamine (METH)-induced neuronal damage. 13 More importantly, a previous report shows that circ_0006916 expression is enhanced in HCC tissues when compared to non-tumor tissues. 14 Furthermore, circ_0006916 can promote HCC cell proliferation, migration and invasion via modulating miR-1322/C-X-C motif chemokine ligand 6 (CXCL6) axis.…”

Section: Introductionmentioning

confidence: 94%

<p>Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis</p>

Zhu

Shen

Zhao

et al. 2020

OTT

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Background The aberrantly expressed circular RNAs (circRNAs) are implicated in the progression of hepatocellular carcinoma (HCC). CircRNA hsa_circ_0006916 (circ_0006916) is dysregulated in HCC, but the function and mechanism of this circRNA in HCC development remain uncertain. Methods Thirty paired HCC and normal tissues were collected. circ_0006916, microRNA (miR)-599 and serine/arginine rich splicing factor 2 ( SRSF2 ) abundances were examined via quantitative reverse transcription polymerase chain reaction or Western blot. Cell viability, colony ability, migration, invasion, cell cycle and apoptosis were tested via cell counting kit-8, colony formation, wound healing analysis, transwell analysis, and flow cytometry. The interaction between miR-599 and circ_0006916 or SRSF2 was analyzed via dual-luciferase reporter and RNA immunoprecipitation analyses. The function of circ_0006916 on cell growth in vivo was analyzed via xenograft model. Results circ_0006916 expression was increased in HCC tissues and cell lines. circ_0006916 knockdown reduced cell viability, colony formation, migration and invasion and caused cell cycle arrest and apoptosis. miR-599 was targeted via circ_0006916, and miR-599 knockdown reversed the influence of circ_0006916 silence on HCC progression. SRSF2 was targeted via miR-599, and miR-599 overexpression suppressed cell viability, colony formation, migration and invasion and promoted cell cycle arrest and apoptosis via decreasing SRSF2 . circ_0006916 could regulate SRSF2 expression via miR-599. circ_0006916 knockdown decreased HCC cell growth in the xenograft model. Conclusion circ_0006916 knockdown represses the progression of HCC via regulating miR-599 and SRSF2 .

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Section: Introductionmentioning

confidence: 94%

<p>Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis</p>

Zhu

Shen

Zhao

et al. 2020

OTT

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“…Circular RNAs are endogenous non-coding RNAs with widespread distribution and various cellular function ( Hansen et al, 2013 ; Vicens and Westhof, 2014 ; Rybak-Wolf et al, 2015 ; Du et al, 2017 ; Greene et al, 2017 ; Li et al, 2018 ; Mehta et al, 2020 ). Numerous studies have shown that circRNAs have an important influence on many complicated neuropsychiatric disorders ( Cui et al, 2016 ; Zhang et al, 2018 ; An et al, 2019 ; Liu et al, 2019 ; Mahmoudi et al, 2019 ; Huang et al, 2020 ; Zhang Y. et al, 2020 ; Zimmerman et al, 2020 ), including drug addiction ( Huang et al, 2017 ; Bu et al, 2019 ; Li et al, 2019 , 2020 ; Zhang H. et al, 2020 ). Knockdown of circHomer1 ameliorates methamphetamine-induced neuronal injury through inhibiting Bbc3 expression ( Li et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have shown that circRNAs have an important influence on many complicated neuropsychiatric disorders ( Cui et al, 2016 ; Zhang et al, 2018 ; An et al, 2019 ; Liu et al, 2019 ; Mahmoudi et al, 2019 ; Huang et al, 2020 ; Zhang Y. et al, 2020 ; Zimmerman et al, 2020 ), including drug addiction ( Huang et al, 2017 ; Bu et al, 2019 ; Li et al, 2019 , 2020 ; Zhang H. et al, 2020 ). Knockdown of circHomer1 ameliorates methamphetamine-induced neuronal injury through inhibiting Bbc3 expression ( Li et al, 2020 ). The abnormal expression of mmu_circRNA_002381 in striatum was induced by cocaine self-administration and cocaine-induced locomotor activity model ( Bu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, siRNA-mediated mmu_circRNA_002381 down-regulation increased the expressions of limk1 and bdnf , which are the targets of miR-138 associated with synaptic plasticity. Additionally, some studies predicted that circRNAs are involved in the progress and development of many addictive drug models by sponging miRNA to regulate downstream targets ( Li et al, 2017 , 2020 ; Bu et al, 2019 ; Zhang H. et al, 2020 ). In our study, 24 circRNAs were identified to be involved in the circRNA–miRNA–mRNA regulatory network.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Functional CircRNA–miRNA–mRNA Regulatory Network in Dorsolateral Prefrontal Cortex Neurons of Patients With Cocaine Use Disorder

Chen

Meng

et al. 2022

Front. Mol. Neurosci.

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Increasing evidence has indicated that circular RNAs (circRNAs) act as competing endogenous RNAs (ceRNAs) regulatory network to regulate the expression of target genes by sponging microRNAs (miRNAs), and therefore play an essential role in many neuropsychiatric disorders, including cocaine use disorder. However, the functional roles and regulatory mechanisms of circRNAs as ceRNAs in dorsolateral prefrontal cortex (dlPFC) of patients with cocaine use disorder remain to be determined. In this study, an expression profiling for dlPFC in 19 patients with cocaine use disorder and 17 controls from Gene Expression Omnibus datasets was used for the differentially expressed circRNAs analysis and the differentially expressed mRNAs analysis. Several tools were used to predict the miRNAs targeted by the circRNAs and the miRNAs targeted mRNAs, which then overlapped with the cocaine-associated differentially expressed mRNAs to determine the functional roles of circRNAs. Functional analysis for the obtained mRNAs was performed via Gene Ontology (GO) in Metascape database. Integrated bioinformatics analysis was conducted to further characterize the circRNA–miRNA–mRNA regulatory network and identify the functions of distinct circRNAs. We found a total of 41 differentially expressed circRNAs, and 98 miRNAs were targeted by these circRNAs. The overlapped mRNAs targeted by the miRNAs and the differentially expressed mRNAs constructed a circRNA–miRNA–mRNA regulation network including 24 circRNAs, 43 miRNAs, and 82 mRNAs in the dlPFC of patients with cocaine use disorder. Functional analysis indicated the regulation network mainly participated in cell response-related, receptor signaling-related, protein modification-related and axonogenesis-related pathways, which might be involved with cocaine use disorder. Additionally, we determined four hub genes (HSP90AA1, HSPA1B, YWHAG, and RAB8A) from the protein–protein interaction network and constructed a circRNA–miRNA-hub gene subnetwork based on the four hub genes. In conclusion, our findings provide a deeper understanding of the circRNAs-related ceRNAs regulatory mechanisms in the pathogenesis of cocaine use disorder.

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“…Drug addiction is a chronic disease, which is accompanied by high relapse rates, compulsive drug-seeking, and forceful withdrawal reactions [1]. Addictive drugs, including methamphetamine (MA), as one of the addictive drugs of abuse, have been a global health threat for fairly long time [2]. The location of the lateral hypothalamus (LH) is in the midbrain region partly and plays a crucial part in maintaining physiological and behavioral homeostasis, which has been involved in reward course and addictive behaviors, particularly in regulating diet [3].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of the Lateral Hypothalamus Attenuates Methamphetamine-Induced Conditioned Place Preference through Regulation of Kcnq3 Expression

Liu

Tan

Liu

et al. 2022

IJMS

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Repeated administration of methylamphetamine (MA) induces MA addiction, which is featured by awfully unpleasant physical and emotional experiences after drug use is terminated. Neurophysiological studies show that the lateral hypothalamus (LH) is involved in reward development and addictive behaviors. Here, we show that repeated administration of MA activates the expression of c-Fos in LH neurons responding to conditioned place preference (CPP). Chemogenetic inhibition of the LH can disrupt the addiction behavior, demonstrating that the LH plays an important role in MA-induced reward processing. Critically, MA remodels the neurons of LH synaptic plasticity, increases intracellular calcium level, and enhances spontaneous current and evoked potentials of neurons compared to the saline group. Furthermore, overexpression of the potassium voltage-gated channel subfamily Q member 3 (Kcnq3) expression can reverse the CPP score and alleviate the occurrence of addictive behaviors. Together, these results unravel a new neurobiological mechanism underlying the MA-induced addiction in the lateral hypothalamus, which could pave the way toward new and effective interventions for this addiction disease.

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Knockdown of circHomer1 ameliorates METH-induced neuronal injury through inhibiting Bbc3 expression

Cited by 16 publications

References 39 publications

<p>Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis</p>

<p>Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis</p>

Identification of Functional CircRNA–miRNA–mRNA Regulatory Network in Dorsolateral Prefrontal Cortex Neurons of Patients With Cocaine Use Disorder

Inactivation of the Lateral Hypothalamus Attenuates Methamphetamine-Induced Conditioned Place Preference through Regulation of Kcnq3 Expression

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