Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing mRNA for calcium-binding protein S100A11

Shin, Heegwon; Lee, Jungmin; Kim, Youngmi; Jang, Seonghui; Lee, Yunhee; Kim, Semi; Lee, Younghoon

doi:10.1080/15476286.2017.1297913

Cited by 25 publications

(28 citation statements)

References 48 publications

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“…The present results showed that BC200 KO inhibited the migration of HepG2 cells, and high expression of BC200 promoted the migration of HepG2 cells. The present results are in line with a recent study indicating that knockdown of BC200 RNA expression reduces cell migration and invasion (29).…”

Section: Discussionsupporting

confidence: 93%

Effect of lncRNA‑BC200 on proliferation and migration of liver cancer cells in�vitro and in�vivo

et al. 2019

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In recent years, the important role of long noncoding RNAs (lncRNAs) in the development of liver cancer has received increasing attention. The abnormal expression level of long non-coding RNAs has been associated with the occurrence and development of liver cancer. However, the role and molecular mechanisms of lncRNAs in the development and progression of liver cancer are not fully understood. The present study aimed to clarify the function and molecular mechanism of lncRNA brain cytoplasmic 200 (BC200) in liver cancer. In the present study, it was found that BC200 expression level was higher in hepatocellular carcinoma (HCC) tissues than that in adjacent tissues. Cell function was examined by constructing BC200 knockout (KO) and BC200-overexpression in vitro models. It was found that BC200 affected the proliferation and migration of HepG2 cells. Interestingly, it was found that BC200 affected the expression of c-Myc protein but did not affect the mRNA expression level of c-MYC. BC200 KO cells exhibited a reduced protein expression level of Bax protein and an increased protein expression level of Bcl-xL. Conversely, BC200 overexpression reduced the expression of Bcl-xL protein and increased the expression of Bax protein. Importantly, it was found that BC200 affected the formation of subcutaneous tumors in nude mice. In conclusion, the present results suggested that lncRNA BC200 may play an important role in liver cancer.

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Section: Discussionsupporting

confidence: 93%

Effect of lncRNA‑BC200 on proliferation and migration of liver cancer cells in�vitro and in�vivo

et al. 2019

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“…Accordingly, BC200 RNA could alter gene expression patterns in very specific ways. A recent ribosome profiling analysis showed that, of 29 genes whose ribosomal occupation was altered more than 2-fold by BC200 knockdown, 16 were upregulated and 13 were downregulated (30). The fact that BC200 RNA alters only a limited number of genes in vivo suggests the existence of an additional mechanism that confers such specificity in vivo.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of RNA Motifs Essential for BC200 RNA-mediated Translational Regulation

2020

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Brain cytoplasmic 200 RNA (BC200 RNA) is proposed to act as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translationalinhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation. [BMB Reports 2020; 53(2): 94-99] BMB Rep. 2020; 53(2): 94-99 www.bmbreports.org

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“…Knockdown of BC200 decreases cell migration in cervical carcinoma and breast cancer cell lines [26]. BC200 acts as an oncogene with a negative effect on cell death; in this regard, the silencing of BC200 could induce obvious G0/G1 arrest, cause apoptosis, and reduce invasion in colon cancer cell lines (i.e., HCT-116 and HT29) [27].…”

Section: Discussionmentioning

confidence: 99%

Effect of CCT137690 on long non-coding RNA expression profiles in MCF-7 and MDA-MB-231 cell lines

Okcanoğlu¹,

Kayabaşı²,

Gündüz³

2019

Bosn J of Basic Med Sci

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Long non-coding RNAs (lncRNAs) are involved in a range of biological processes, such as cellular differentiation, migration, apoptosis, invasion, proliferation, and transcriptional regulation. The aberrant expression of lncRNAs plays a significant role in several cancer types. Aurora kinases are increasingly expressed in various malignancies; accordingly, the inhibition of these enzymes may represent a novel approach for the treatment of various cancers. CCT137690, an Aurora kinase inhibitor, displays an anti-proliferative activity in human cancer cell lines. The aim of the present study was to investigate the anti-proliferative and cytotoxic effects of CCT137690 on estrogen receptor (ER)-positive human breast cancer cell line (MCF-7) and ER-negative human breast cancer cell line (MDA-MB-231). In addition, this study was targeted toward determining the changes induced in lncRNA expression levels following the initiation of Aurora kinase inhibitor treatment. The cytotoxic effects of CCT137690 were determined by means of the xCELLigence system. Furthermore, the anti-proliferative role of CCT137690 in breast cancer was investigated by checking the changes in lncRNA expression profiles using quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The half-maximal inhibitory concentrations (IC50) of CCT137690 were determined as 4.5 μM (MCF-7) and 7.27 μM (MDA-MB-231). Several oncogenic lncRNAs (e.g., PRINS, HOXA1AS, and NCRMS) were downregulated in both ER-negative and ER-positive cell lines. On the other hand, tumor suppressor lncRNAs (e.g., DGCR5 and IGF2AS) were upregulated in the ER-positive cell line. After CCT137690 treatment, HOXA11AS and PCAT-14 lncRNAs were downregulated in the ER-positive cell lines. In addition, MER11C, SCA8, BC200, HOTAIR, PCAT-1, UCA1, SOX2OT, and HULC lncRNAs were downregulated in the ER-negative cell lines. The results of the present study indicated that Aurora kinase inhibitor CCT137690 could be a potential anti-cancer agent for breast cancer treatment.

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Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing mRNA for calcium-binding protein S100A11

Cited by 25 publications

References 48 publications

Effect of lncRNA‑BC200 on proliferation and migration of liver cancer cells in�vitro and in�vivo

Effect of lncRNA‑BC200 on proliferation and migration of liver cancer cells in�vitro and in�vivo

Functional Analysis of RNA Motifs Essential for BC200 RNA-mediated Translational Regulation

Effect of CCT137690 on long non-coding RNA expression profiles in MCF-7 and MDA-MB-231 cell lines

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