Knockdown of ASH1L methyltransferase induced apoptosis inhibiting proliferation and H3K36 methylation in bovine cumulus cells

Cui, Linlin; Tian, Ya-Qing; Huang, Hao; Zou, Huiying; Pang, Yuxin; Zhao, Shanjiang; Zhao, Xiao‐Fan; Zhu, Huabin; Du, Weihua

doi:10.1016/j.theriogenology.2020.11.007

Cited by 8 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nuclear staining with 4′,6-diamidino-2-phenylindole was performed at room temperature for 15 min, followed by five washings with PBST for 5 min. Finally, the sections were sealed and examined under a fluorescence microscope ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptomics reveals age-related changes in ion transport–related factors in yak lungs

Xie,

Wei,

Cui

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

Yaks inhabit high-altitude, low-oxygen regions, where ion transport functions play a crucial role in maintaining intracellular and extracellular ionic balance and regulating pulmonary vascular tension. These functions affect pulmonary ventilation and blood flow rate, aiding tissue development and enhancing oxygen transfer efficiency, thus facilitating better adaptation to hypoxic environments. To investigate the regulatory mechanisms of ion transport-related factors on the growth and development of yak lungs, we employed RNA sequencing (RNA-seq)for sequencing the transcriptome in the lung tissues of neonatal (1-day-old), juvenile (1-year-old), and adult (4-year-old) yaks. We also performed differential gene expression and functional analyses. The results yielded 26 genes associated with ion transport, mainly enriched in the salivary and pancreatic secretion pathways. Finally, we used several methods including quantitative polymerase chain reaction (qRT-PCR), and Western blotting (WB), immunohistochemical (IHC) and immunofluorescence (IF) staining to determine the distribution of the expression of the ion transport genes FOXI1, KCNMA1, and SLC12A2 in yak lung tissues. qRT-PCR and WB results indicated that mRNA and protein relative expression levels of FOXI1 and SLC12A2 were significantly higher in neonatal yaks than in juvenile and adult yaks (all p < 0.05), whereas those of KCNMA1 were significantly higher in adult yaks than in neonatal and juvenile yaks (all p < 0.05). IHC and IF results demonstrated that FOXI1, KCNMA1, and SLC12A2 were distributed among the epithelial mucosal layers (including ciliated, goblet, and Clara cells) of the yaks’ bronchi and their branches in the lungs across different age groups of yak. Therefore, our results suggested that FOXI1, KCNMA1, and SLC12A2 may be strongly associated with the development and aging processes in yak lungs. These results provide insights into the molecular mechanisms underlying the yak’s adaptation to high-altitude environments and valuable references for further research.

show abstract

Section: Methodsmentioning

confidence: 99%

Transcriptomics reveals age-related changes in ion transport–related factors in yak lungs

Xie,

Wei,

Cui

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…After the ovaries were washed three times with sterile saline containing 100 IU/mL penicillin and 100 mg/mL streptomycin sulfate, cumulus–oocyte complexes (COCs) were aspirated from the healthy antral follicles [ 22 ]. They were then transferred to a petri dish.…”

Section: Methodsmentioning

confidence: 99%

Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells

Wang²,

Wang³

et al. 2022

Animals

View full text Add to dashboard Cite

Exosomes in the follicular fluid can carry and transfer regulatory molecules to recipient cells, thus influencing their biological functions. However, the specific effects of yak follicular fluid exosomes on 2-hydroxyestrodiol (2-OHE2) secretion remain unknown. Here, we investigated whether yak follicular fluid exosomes can increase 2-OHE2 secretion through the activation of autophagy in cumulus cells (YCCs). In vitro cultured YCCs were treated with yak follicular fluid exosomes for 6, 12, and 24 h. The effects of yak follicular fluid exosomes on autophagy and 2-OHE2 secretion were evaluated through real-time quantitative fluorescence PCR (RT-qPCR), Western blotting (WB), transfected with RFP-GFP-LC3, immunohistochemistry, and ELISA. To further investigate whether 2-OHE2 secretion was related to autophagy, YCCs were administered with yak follicular fluid exosomes, 3-methyladenine (3-MA), and rapamycin (RAPA). The results revealed that treatment with yak follicular fluid exosomes activated autophagy in YCCs and increased 2-OHE2 secretion. Conversely, the inhibition of autophagy with 3-MA blocked these effects, suggesting that autophagy has an important role in 2-OHE2 secretion in YCCs. Treatment of YCCs with rapamycin showed similar results with yak follicular fluid exosomes as there was an increase in 2-OHE2 secretion due to the activation of autophagy in the treated cumulus cells. Our results demonstrate that autophagy is enhanced by yak follicular fluid exosomes, and this is associated with an increase in 2-OHE2 secretion in YCCs.

show abstract

“…It was reported that Ash1l mutant mice exhibit partially postnatal lethality and other mutant mice that survive have growth insufficiency and infertility caused by defects in both epididymis and uterine development in male and female mice ( 23 ). Studies demonstrated that knockdown of Ash1l in bovine cumulus cells induced a decrease in the levels of H3K36me1/2/3, inhibited proliferation, and upregulated the expression of apoptosis ( 24 ). However, the role of ASH1L in meiotic prophase I and early folliculogenesis during the fetal stage remains unclear in mammals.…”

Section: Introductionmentioning

confidence: 99%

ASH1L contributes to oocyte apoptosis by regulating DNA damage

Zhang

Ren

Lin

et al. 2022

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

In female mammals, the size of the initially established primordial follicle pool within the ovaries determines the reproductive lifespan of females. Interestingly, the establishment of the primordial follicle pool is accompanied by a remarkable programmed oocyte loss for unclear reasons. Here, we identify a new role of ASH1 like histone lysine methyltransferase (ASH1L) in controlling the apoptosis of oocytes during meiotic prophase I in mice. Our results showed that overexpression of Ash1l led to a dramatic loss of fetal oocytes via apoptosis, which subsequently resulted in a reduced capacity of the primordial follicle pool. Overexpression of Ash1l also led to a deficiency in DNA double-strand break repair associated with premature upregulation of TAp63 and p-CHK2, the major genome guardian of the female germline, following Ash1l overexpression in fetal ovaries. In summary, ASH1L was one of indispensible epigenetic molecules who acts as a guardian of the genome. It protects oocytes genome integrity and remove oocytes with seriously DNA damage by regulating the expression of TAp63 and p-CHK2 during meiotic prophase I in mice. Our study provides a perspective on the physiological regulatory role of DNA damage checkpoint signaling in fetal oocyte guardianship and female fertility.

show abstract

Knockdown of ASH1L methyltransferase induced apoptosis inhibiting proliferation and H3K36 methylation in bovine cumulus cells

Cited by 8 publications

References 37 publications

Transcriptomics reveals age-related changes in ion transport–related factors in yak lungs

Transcriptomics reveals age-related changes in ion transport–related factors in yak lungs

Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells

ASH1L contributes to oocyte apoptosis by regulating DNA damage

Contact Info

Product

Resources

About