Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis

Sangsuriya, Pakkakul; Phiwsaiya, Kornsunee; Pratoomthai, Benjamart; Sriphaijit, Thanawat; Amparyup, Piti; Withyachumnarnkul, Boonsirm; Senapin, Saengchan

doi:10.1016/j.fsi.2014.01.007

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…An earlier report showed that knockdown of a novel G-protein pathway suppressor 2 ( GPS2 ) (GenBank accession number JN714124) led to mortality by exuvial entrapment during ecdysis in shrimp ( Penaeus vannamei ) [ 86 ]. In our study, we identified a GPS2 gene, c74297_g2, and its expression exhibited low variance levels; this gene was thought to be a housekeeping gene that acts during the molting cycle in L .…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis Provides Insights into Molecular Mechanisms for Molting in Litopenaeus vannamei

et al. 2015

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Molting is one of the most important biological processes in shrimp growth and development. All shrimp undergo cyclic molting periodically to shed and replace their exoskeletons. This process is essential for growth, metamorphosis, and reproduction in shrimp. However, the molecular mechanisms underlying shrimp molting remain poorly understood. In this study, we investigated global expression changes in the transcriptomes of the Pacific white shrimp, Litopenaeus vannamei, the most commonly cultured shrimp species worldwide. The transcriptome of whole L. vannamei was investigated by RNA-sequencing (RNA-seq) throughout the molting cycle, including the inter-molt (C), pre-molt (D0, D1, D2, D3, D4), and post-molt (P1 and P2) stages, and 93,756 unigenes were identified. Among these genes, we identified 5,117 genes differentially expressed (log2ratio ≥1 and FDR ≤0.001) in adjacent molt stages. The results were compared against the National Center for Biotechnology Information (NCBI) non-redundant protein/nucleotide sequence database, Swiss-Prot, PFAM database, the Gene Ontology database, and the Kyoto Encyclopedia of Genes and Genomes database in order to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. The expression patterns for genes involved in several molecular events critical for molting, such as hormone regulation, triggering events, implementation phases, skelemin, immune responses were characterized and considered as mechanisms underlying molting in L. vannamei. Comparisons with transcriptomic analyses in other arthropods were also performed. The characterization of major transcriptional changes in genes involved in the molting cycle provides candidates for future investigation of the molecular mechanisms. The data generated in this study will serve as an important transcriptomic resource for the shrimp research community to facilitate gene and genome annotation and to characterize key molecular processes underlying shrimp development.

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Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis Provides Insights into Molecular Mechanisms for Molting in Litopenaeus vannamei

et al. 2015

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“…Several genes related to chitin synthsis 26 , degradation 27 , and mineral absorption 28 were amplified and sequenced. RNA interference showed that some factors may be associated with exoskeleton reconstruction, such as CqGAP10 29 , CqGAP65 30 , LvGPS2 31 , CqMIH 32 . However, the information collected using these methods is incomplete and provided only a fragmented picture.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

Gao

Wei

Yuan

et al. 2017

Sci Rep

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Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology, Clusters of Orthologous Group (COG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and functional annotation of all unigenes gave a better understanding of the exoskeleton developmental process in L. vannamei. As a result, more than six hundred unigenes related to exoskeleton development were identified both in the early developmental stages and adult-moulting. A cascade of sequential expression events of exoskeleton-related genes were summarized, including exoskeleton formation, regulation, synthesis, degradation, mineral absorption/reabsorption, calcification and hardening. This new insight on major transcriptional events provide a deep understanding for exoskeleton formation and reconstruction in L. vannamei. In conclusion, this is the first study that characterized the integrated transcriptomic profiles cover the entire exoskeleton development from zygote to adult-moulting in a crustacean, and these findings will serve as significant references for exoskeleton developmental biology and aquaculture research.

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“…This could be related with large influence of, among others, food quality on the activity of hormones regulating insect physiology, such as juvenile and prothoracic hormones which affect arthropod growth and moulting (Lee et al 2012;Andersen et al 2013;Nijhout et al 2014;Sangsuriya et al 2014). In our study, L1 grubs showed the lowest vitality, which was probably due to the presence of pathogens in the population collected in the field (high L1 grub mortality was observed already during the transfer to the laboratory).…”

Section: Discussionmentioning

confidence: 99%

Survival and growth of the Melolontha spp. grubs on the roots of the main forest tree species

Woreta

Sukovata

2015

Forest Research Papers

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Abstract. The survival, weight and relative growth rate (RGR) of the Melolontha spp. grubs feeding on roots of Quercus petraea, Q. robur, Fagus sylvatica, Betula pendula, Larix decidua, Alnus glutinosa and Pinus sylvestris, were examined.Overall, the youngest grubs L1 were the most affected by food quality. The mortality of the grubs feeding on the roots of A. glutinosa changed most rapidly and, consequently, LT50 was the shortest (25.9 days), whereas the slowest changes in mortality with the highest LT50 values were observed on the two oak species (54.9 and 44.9 days on Q. robur and Q. petraea, respectively). The RGRs of the L1 grubs were the highest on oaks, F. sylvatica and B. pendula. The overall rate of survival of the older grubs was high (66.7-100%). It was the lowest on the roots of B. pendula (L2 grubs) and L. decidua (L3 grubs), which at the same time displayed the highest RGRs.The interpretation of the results is difficult due to the lack of basic knowledge on the potential effects of food quality and other factors on grub metamorphosis. There is no doubt, however, that among the seven tested tree species the roots of A. glutinosa are the least favorable for the Melolontha grubs' performance.

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Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis

Cited by 5 publications

References 36 publications

Whole Transcriptome Analysis Provides Insights into Molecular Mechanisms for Molting in Litopenaeus vannamei

Whole Transcriptome Analysis Provides Insights into Molecular Mechanisms for Molting in Litopenaeus vannamei

Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

Survival and growth of the Melolontha spp. grubs on the roots of the main forest tree species

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