Knockdown Epithelial Membrane Protein 1 Suppresses Human Degenerative Intervertebral Disc–Derived Nucleus Pulposus Cell Proliferation

Li, Zhi-Yong; Xiong, Shao-Hu; Hu, Ming; Zhang, Chuansen

doi:10.1177/1947603510392022

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…In these cancers high endogenous EMP1 expression correlates with poor patients’ outcome. In line with these findings, silencing EMP1 in chondrocytes inhibits cell proliferation [41].…”

Section: Discussionmentioning

confidence: 71%

p53, miR-34a and EMP1—Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells

Busch

Klein

Große-Kreul

et al. 2019

IJMS

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Trefoil factor family peptide 3 (TFF3) is supposed to have tumor suppressive functions in retinoblastoma (RB), but the functional pathway is not completely understood. In the study presented, we investigated the downstream pathway of TFF3 signaling in Y79 RB cells. Results from pG13-luciferase reporter assays and western blot analyses indicate induced p53 activity with an upregulation of miR-34a after TFF3 overexpression. Expression levels of the predicted miR-34a target epithelial membrane protein 1 (EMP1) are reduced after TFF3 overexpression. As revealed by WST-1 assay, BrdU, and DAPI cell counts viability and proliferation of Y79 cells significantly decrease following EMP1 knockdown, while apoptosis levels significantly increase. Opposite effects on Y79 cells’ growth could be shown after EMP1 overexpression. Caspase assays showed that EMP1 induced apoptosis after overexpression is at least partially caspase-3/7 dependent. Colony formation and soft agarose assays, testing for anchorage independent growth, revealed that EMP1 overexpressing Y79 cells have a significantly higher ability to form colonies. In in ovo chicken chorioallantoic membrane (CAM) assays inoculated EMP1 overexpressing Y79 cells form significantly larger CAM tumors. Moreover, miR-34a overexpression increases sensitivity of Y79 cells towards RB chemotherapeutics, however, without involvement of EMP1. In summary, the TFF3 signaling pathway in Y79 RB cells involves the activation of p53 with downstream induction of miR-34a and subsequent inhibition of EMP1.

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“…In these cancers high endogenous EMP1 expression correlates with poor patients’ outcome. In line with these findings, silencing EMP1 in chondrocytes inhibits cell proliferation [41].…”

Section: Discussionmentioning

confidence: 71%

p53, miR-34a and EMP1—Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells

Busch

Klein

Große-Kreul

et al. 2019

IJMS

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“…NPCs are one of the most important components of IDD. NPCs proliferation inhibition and apoptosis directly induce IDD [19][20][21]; thus, the molecular mechanism of NPCs proliferation and apoptosis should be closely related to IDD pathogenesis. In this study, 52 differentially expressed miRNAs were identi ed with |fold change| >2 and P < 0.05.…”

Section: Discussionmentioning

confidence: 99%

miR-338-3p inhibits HIF-1α to Attenuate Nucleus Pulposus Cell Proliferation and Promote Apoptosis Through the Hippo-YAP Pathway

Zhan

Liu

et al. 2021

Preprint

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The proliferation and apoptosis of nucleus pulposus (NP) cells (NPCs) play a crucial role in intervertebral disc degeneration (IDD). we aimed to discover the role of miRNA-induced IDD. We analyzed the miRNA expression of three NP tissues from IDD patients and three normal NP samples using the GEO2R tool, and The results revealed that miR-338-3p was upregulated in NPCs from IDD patients. miR-338-3p suppressed NPCs proliferation, and the related proteins PCNA and Ki-67 were downregulated, as demonstrated via western blotting. miR-338-3p promoted apoptosis. Furthermore, we predicted that HIF-1α was targeted by miR-338-3p, using the miRDB database, and this target was validated via dual luciferase assay. HIF-1α reversed miR-338-3p-induced NPCs proliferation and apoptosis. The Hippo-YAP pathway activation proteins YAP, CTGF, and PCNA were upregulated, unlike the inhibitory YAP phosphorylation. In conclusions, our results suggestive that miR-338-3p inhibited HIF-1α/ Hippo-YAP pathway to attenuate NPCs proliferation and apoptosis.

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“…Epithelial membrane protein 1 ( EMP-1 ) gene belongs to the peripheral myelin protein ( PMP )-22 family, also named as CL-40 , tumor-associated membrane protein ( TMP ), and B4B [ 8 , 9 ]. It has been reported that EMP-1 plays an important role in various cellular processes.…”

Section: Introductionmentioning

confidence: 99%

The Prognostic Value of Epithelial Membrane Protein 1 (EMP-1) in Patients with Laryngeal Carcinoma

Liu

Wei

et al. 2017

Med Sci Monit

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BackgroundIn the present study, we aimed to investigate the prognostic value of epithelial membrane protein 1 (EMP-1) gene in patients diagnosed with laryngeal carcinoma (LC).Material/MethodsPatients who were pathologically diagnosed with LC were enrolled in the present study. The expression levels of EMP-1 in tumor tissues and corresponding normal tissues collected from the LC patients were detected by semi-reverse transcriptase polymerase chain reaction (semi-RT-PCR). Chi-square analysis was used to evaluate the relationship between EMP-1 expression level and clinical characteristics. Survival analysis for the study population was analyzed by Kaplan-Meier method with log rank test. Additionally, Cox regression model was applied to evaluate the prognostic value of EMP-1 in LC patients.Results106 LC patients, including 55 men and 51 women, were enrolled in the present study. Semi-RT-PCR demonstrated that the expression level of EMP-1 was decreased in tumor tissues, compared with adjacent normal tissues (p<0.001). Moreover, the level was significantly associated with lymph node metastasis, histological grade, and clinical stage (p<0.05 for all). In addition, low levels of EMP-1 was significantly correlated with poor survival rate (log rank test, p=0.020). Cox regression analysis indicated that EMP-1 was an independent marker for LC prognosis (HR=2.755, 95% CI=1.123–6.760, p=0.027).ConclusionsThe abnormal expression of EMP-1 may be associated with progression of LC and the gene may act as a prognostic marker for LC.

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Knockdown Epithelial Membrane Protein 1 Suppresses Human Degenerative Intervertebral Disc–Derived Nucleus Pulposus Cell Proliferation

Cited by 4 publications

References 18 publications

p53, miR-34a and EMP1—Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells

p53, miR-34a and EMP1—Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells

miR-338-3p inhibits HIF-1α to Attenuate Nucleus Pulposus Cell Proliferation and Promote Apoptosis Through the Hippo-YAP Pathway

The Prognostic Value of Epithelial Membrane Protein 1 (EMP-1) in Patients with Laryngeal Carcinoma

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