Knock-Down of Endogenous Bornavirus-Like Nucleoprotein 1 Inhibits Cell Growth and Induces Apoptosis in Human Oligodendroglia Cells

He, Peng; Sun, Lin; Zhu, Dan; Zhang, Hong; Zhang, Liang; Guo, Yujie; Liu, Siwen; Zhou, Jingjing; Xu, Xiaoyan; Xie, Peng

doi:10.3390/ijms17040435

Cited by 23 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Golgin45 was also demonstrated to interact with DNA (cytosine-5)-methyltransferase 3-like protein DNMT3L and AP-1 (9,10). Gene profile analysis found that Golgin45 expression was up-regulated in liver cells that responded to dengue virus infection (11), in transcription factor Gfi-1B (growth factor independence 1B) overexpressing leukemia cells (12), and in EBLN1 (Endogenous bornavirus-like nucleoprotein elements) silencing oligodendroglia cells (13), and it was reduced in hepatocellular carcinoma tissues (14). The exact role of Golgin45 still needs further confirmation and investigation.…”

mentioning

confidence: 99%

Structural Basis for the Interaction between Golgi Reassembly-stacking Protein GRASP55 and Golgin45

Zhao

Huang

et al. 2017

Journal of Biological Chemistry

View full text Add to dashboard Cite

Golgin45 is required for normal Golgi structure and the transportation of protein from the ER. It forms a specific complex with GRASP55 Little is known regarding the molecular details of this interaction and its structural role in stacking of the Golgi complex. Here, we present the crystal structure of the GRASP domains of GRASP55 in complex with the Golgin45 C-terminal peptide, determined at 1.33 Å resolution. Similar to the structure of GRASP65 bound to GM130 reported recently, this structure reveals more than one interacting site and involves both PDZ1 and PDZ2 domains of the GRASP simultaneously. The C-terminal peptides of Golgin45 and GM130 present a conserved PDZ domain binding motif sequence and recognize the canonical PDZ-peptide binding groove of the PDZ1 domains of GRASP55 and GRASP65. A main difference in this recognition process resides in a structural rearrangement of GRASP65-GM130 that does not occur for the GRASP55-Golgin45 complex. The binding site at the cleft between the PDZ1 and PDZ2 domains of GRASP65 is dominated by hydrophobic interactions with GM130 that are not observed in the GRASP55-Golgin45 complex. In addition, a unique zinc finger structure is revealed in the GRASP55-Golgin45 complex crystal structure. Mutagenesis experiments support these structural observations and demonstrate that two of these sites are required to form a stable complex. Finally, a novel Golgi stacking model is proposed according to these structural findings.

show abstract

mentioning

confidence: 99%

Structural Basis for the Interaction between Golgi Reassembly-stacking Protein GRASP55 and Golgin45

Zhao

Huang

et al. 2017

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Because no natural selection of hsEBLN-1 and its orthologues is detected [54], hsEBLN-1 is thought to function as a DNA element or non-coding RNA, or even to have lost its function (Figure 3). He et al reported that 1067 and 2004 genes are up-and downregulated, respectively, after knockdown of hsE-BLN-1 RNA in human oligodendroglia cells [55]. The top 10 most upregulated genes were PI3, RND3, BLZF1, SOD2, EPGN, SBSN, INSIG1, OSMR, CREB3L2, and MSMO1, and the top 10 most downregulated genes were KRTAP2-4, FLRT2, DIDO1, FAT4, ESCO2, ZNF804A, SUV420H1, ZC3H4, YAE1D1, and NCOA5.…”

Section: Gene Regulation By Ebln Rnasmentioning

confidence: 99%

“…The top 10 most upregulated genes were PI3, RND3, BLZF1, SOD2, EPGN, SBSN, INSIG1, OSMR, CREB3L2, and MSMO1, and the top 10 most downregulated genes were KRTAP2-4, FLRT2, DIDO1, FAT4, ESCO2, ZNF804A, SUV420H1, ZC3H4, YAE1D1, and NCOA5. Gene ontology revealed that hsEBLN-1 may regulate the expression of genes related to the cell cycle, the mitogen-activated protein kinase pathway, p53 signaling, and apoptosis [55].…”

Section: Gene Regulation By Ebln Rnasmentioning

confidence: 99%

Influence of Endogenous Viral Sequences on Gene Expression

Sofuku¹,

Honda²

2018

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects

View full text Add to dashboard Cite

Endogenous viral elements (EVEs) are the heritable sequences present in eukaryotic genomes that have originated from viral nucleotide sequences. EVEs are subdivided into two groups, according to the presence or absence of long terminal repeats (LTRs). EVEs with LTRs are called endogenous retroviruses (ERVs), and they account for approximately 8% of the human genome. EVEs without LTRs seem to be related to non-reverse-transcribing RNA and DNA viruses, and recent studies have revealed that numerous vertebrate genomes contain these non-LTR EVEs. Such EVEs are proposed to play essential roles in gene expression. EVEs can regulate gene expression as cis-regulatory DNA and RNA elements. EVE-derived non-coding RNAs and/or proteins can also influence cell transcriptomes in trans. To maintain cell integrity, cells epigenetically silence the expression of most EVEs, making these elements generally biochemically inert. These epigenetic alterations around the EVE loci can also affect host transcriptomes. Here, we highlight the current knowledge available on the regulatory activities of ERVs and non-retroviral EVEs, especially the EVEs derived from bornaviruses, which are known as endogenous bornavirus-like elements (EBLs). Better knowledge of this area will improve our understanding of gene regulation and also the co-evolution of viruses and their hosts.

show abstract

“…Найчастіше це філовіруси (зокрема, вірус Ебола) та борнавіруси (вірус хвороби Борна). Одним із можливих механізмів реалізації цих ендогенних борнавірусів може бути їх роль у регуляції функцій клітини, її неконтрольованого розмноження (проліферації) та загибелі (апоптозі) (He et al, 2016). Адже саме борнавіруси мають унікальну здатність інтегруватися у процесі своєї реплікації в геном господаря, тому автори припускають набагато ширше коло природних господарів цих вірусів, а їх біологічне значення потребує подальшого вивчення.…”

Section: значення борнавірусів у патології людиниunclassified

Borna disease virus and its role in the pathology of animals and humans

Mikheev¹

2017

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Infectious diseases that are caused by numerous pathogenic microorganisms – bacteria, viruses, protozoa or fungi – can be transmitted from patients or carriers to healthy people or animals. A large group of infectious disease is caused by pathogens of animal infections – zoonoses. The issue of zoonoses is of great significance in human pathology and requires comprehensive study. This is of particular relevance to Ukraine, as the question of prevalence, level within the population and threats to human life and health from zoonoses, though highly important, has remained insufficiently studied. Information about many of these pathogens is absent in the existing scientific literature accessible in Ukraine – both veterinary and medical. This applies, in particular, to a causative agent of viral zoonoses the Borna disease virus or Bornavirus. For this purpose, an analysis of the literature concerning the role of the Bornavirus in the pathology of animals and humans was conducted. It is well known that a large number of pathogens of animal infections (zoonoses), including viral, pose a potential threat to human health. Among these potential threats is the Borna disease virus belonging to the family of Bornaviridae, order Mononegavirales. This order includes representatives of deadly human diseases like rabies (family Rhabdoviridae), Ebola virus (family Filoviridae) and Nipah virus (family Paramyxoviridae). Borna virus disease affects mainly mammals, but can infect birds and even reptiles (Aspid bornavirus). It is established that Bornaviruses have a wide range of natural hosts (horses, sheeps, cats, bats and various birds), including domestic animals, which poses a potential threat to human health. This is evidenced by numerous, although contradictory, research into the role of the Borna disease virus in human pathologies such as schizophrenia, depression, prolonged fatigue syndrome, multiple sclerosis and others. Analysis of the literature clearly shows the important role of the Borna disease virus in a variety of mental and behavioural changes in animals, both wild and domestic. However, the large amount of contradictory data and studies cannot yet provide a clear picture of the role of this virus in human pathology. On the one hand, there are clear data of the presence of RNA of the Bourna disease virus and antibodies to it in patients with psychoneurotic changes. On the other, there is no clear understanding of the infectivity of the virus in humans and its transmission from animals to humans and from person to person. These questions require further research and comprehensive study, particularly on the territory of Ukraine.

show abstract

Knock-Down of Endogenous Bornavirus-Like Nucleoprotein 1 Inhibits Cell Growth and Induces Apoptosis in Human Oligodendroglia Cells

Cited by 23 publications

References 43 publications

Structural Basis for the Interaction between Golgi Reassembly-stacking Protein GRASP55 and Golgin45

Structural Basis for the Interaction between Golgi Reassembly-stacking Protein GRASP55 and Golgin45

Influence of Endogenous Viral Sequences on Gene Expression

Borna disease virus and its role in the pathology of animals and humans

Contact Info

Product

Resources

About