Knock-down of circular RNA H19 induces human adipose-derived stem cells adipogenic differentiation via a mechanism involving the polypyrimidine tract-binding protein 1

Zhu, Yiyi; Gui, Weiwei; Lin, Xihua; Li, Hong

doi:10.1016/j.yexcr.2019.111753

Cited by 72 publications

(59 citation statements)

References 31 publications

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“…On the one hand, as described above, only a few circRNAs are proved to participate in lipid-related diseases. For example, circH19 is correlated with lipid-related parameters and is a risk factor of metabolic syndrome [109], and cir-cRNA_0010522 is positively linked with the mass of BAT and body fat rate in GC patients [120]. Meanwhile, the number of circRNAs with thorough functions is limited, and the precise mechanisms of majority of circRNAs remain to be elucidated.…”

Section: Therapeutic Potential and Future Perspectivesmentioning

confidence: 99%

Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid‐related diseases

et al. 2020

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Section: Therapeutic Potential and Future Perspectivesmentioning

confidence: 99%

Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid‐related diseases

et al. 2020

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“…Another study by Schmidt et al reported that the overexpression of H19, a maternally imprinted lncRNA that plays a role in lipid metabolism, protects against obesity, and improves insulin sensitivity [81]. In samples from patients with metabolic syndrome, a condition that is associated with abdominal obesity and CVD, the level of hsa_circH19 derived from H19 pre-RNA, was found to be highly increased and significantly correlated with variables of adiposity including body mass index (BMI), waist circumference, fat percent, high-density lipoprotein cholesterol (HDL-c), and visceral fat area [82]. Conversely, silencing of hsa_circH19 promoted human adipose-derived stem cells (hADSCs) adipogenic differentiation presumably through the interaction of such a circRNA with polypyrimidine tract-binding protein 1 (PTBP1) [82].…”

Section: Circrnas In Adipogenesis and Obesitymentioning

confidence: 99%

The Emerging Role and Promise of Circular RNAs in Obesity and Related Metabolic Disorders

Zaiou

2020

Cells

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Circular RNAs (circRNAs) are genome transcripts that are produced from back-splicing of specific regions of pre-mRNA. These single-stranded RNA molecules are widely expressed across diverse phyla and many of them are stable and evolutionary conserved between species. Growing evidence suggests that many circRNAs function as master regulators of gene expression by influencing both transcription and translation processes. Mechanistically, circRNAs are predicted to act as endogenous microRNA (miRNA) sponges, interact with functional RNA-binding proteins (RBPs), and associate with elements of the transcriptional machinery in the nucleus. Evidence is mounting that dysregulation of circRNAs is closely related to the occurrence of a range of diseases including cancer and metabolic diseases. Indeed, there are several reports implicating circRNAs in cardiovascular diseases (CVD), diabetes, hypertension, and atherosclerosis. However, there is very little research addressing the potential role of these RNA transcripts in the occurrence and development of obesity. Emerging data from in vitro and in vivo studies suggest that circRNAs are novel players in adipogenesis, white adipose browning, obesity, obesity-induced inflammation, and insulin resistance. This study explores the current state of knowledge on circRNAs regulating molecular processes associated with adipogenesis and obesity, highlights some of the challenges encountered while studying circRNAs and suggests some perspectives for future research directions in this exciting field of study.

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“…To extract the total proteins, cells were lysed with RIPA Lysis Buffer (Beyotime Biotechnology, Nantong, China) as described previously. 19 The lysate was centrifuged by 12,000 rpm at 4°C for 10 minutes. The supernatant was transferred to a new tube to quantify the protein amount by BCA assay.…”

Section: Western Blotmentioning

confidence: 99%

<p>MiR-1299 Impedes the Progression of Non-Small-Cell Lung Cancer Through EGFR/PI3K/AKT Signaling Pathway</p>

Cao

et al. 2020

OTT

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Background: Non-small-cell lung cancer (NSCLC) is one of the most malignant tumors. In which, numerous miRNAs had been reported to participate in the pathogenesis. However, the expression and function of miR-1299 in NSCLC are not clear. Methods: To explore the roles of miR-1299 in NSCLC, we detected the levels of miR-1299 in clinical samples of NSCLC and investigated the role of miR-1299 in the regulation of the NSCLC cells proliferation, metastasis, and EMT. Luciferase reporter assay was employed to verify the target of miR-1299. Additionally, the proliferation, metastasis, and EMT of A549 and H1299 cells were analyzed after the overexpression and knockdown of miR-1299. Results: We found that the miR-1299 expression negatively corresponded with the clinical stage and overall survival in NSCLC patients. Overexpression of miR-1299 inhibited the migration, invasion, and EMT of A549 and H1975 cells. Meanwhile, we proved that miR-1299 is the sponge of EGFR. Besides, our results suggested that miR-1299 inhibits the progression of NSCLC cells through the PI3K/Akt signal pathway. Conclusion: We demonstrated that miR-1299 inhibits the progression of NSCLC through the EGFR/PI3K/Akt signal pathway. Therapeutic intervention targeting the miR-1299 may provide a potential strategy for the treatment of NSCLC.

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Knock-down of circular RNA H19 induces human adipose-derived stem cells adipogenic differentiation via a mechanism involving the polypyrimidine tract-binding protein 1

Cited by 72 publications

References 31 publications

Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid‐related diseases

Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid‐related diseases

The Emerging Role and Promise of Circular RNAs in Obesity and Related Metabolic Disorders

<p>MiR-1299 Impedes the Progression of Non-Small-Cell Lung Cancer Through EGFR/PI3K/AKT Signaling Pathway</p>

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